Abstract

3 tesla magnetic resonance imaging and multiplanar reconstruction of the brain and its associated structures in sheep

Abstract

The purpose of the study was to scan the brain and related structures in sheep with high-resolution magnetic resonance imaging (MRI) and three-dimensional (3D) multiplanar reconstruction for defining the anatomical regions. Six adult sheep, three of six were male and three of six were female used as cadaver, were utilized in this research. Heads were scanned at 3 Tesla Siemens Magnetom Spin-Echo MRI devices using the human head coil. The processes were acquired in both T1 and T2 weighted slices and were reconstructed by using Leonardo workstation. The cranioencephalic structures and anatomical details were defined and labeled in all slice of 3 planes that were obtained 1 mm thickness sequential images in sagittal, frontal, and transversal planes. According to the MRI measurements obtained intracranially from the brain, the average value of length, height, and width of the brain were 87.1 ± 0.3, 46.8 ± 0.7, and 62.2 ± 0.4 mm, respectively. It was observed that in T1 weighted images were more effective to identify deep brain structures and anatomical details. On 3D reconstructed images obtained from the study can be used as a reference in head and brain MR scans in multidisciplinary studies where sheep are used as animal models.

Keywords

• Animal model,MRI,nervous system,sheep,3D reconstruction

References

1. Avanus K, Altınel A (2016): Identification of Genetic Variation of Melatonin Receptor 1A (MTNR1A) Gene in Kıvırcık Breed Ewes by MnlI and RsaI Restriction Enzymes. Kafkas Univ Vet Fak Derg, 22(4), 571-576.
2. Brachthäuser L, Klumpp S, Hecht W, et al. (2012): Aprosencephaly with otocephaly in a lamb (Ovis aries). Vet Pathol, 49(6), 1043-1048.
3. De Lahunta (2009): Veterinary Neuroanatomy and Clinical Neurology. 3rd Ed. Philadelphia, London, Toronto, Mexico City, Sydney, Tokyo: W.B.Saunders Company.
4. Ekim O, Oto C, Algın O, et al. (2013): High resolution 3D magnetic resonance imaging of the visceral organs in chicken (Gallus domesticus) by 3 Tesla MR unit and 15-channel transmit coil. Vet J Ankara Univ, 60(4), 229-233.
5. Ella A, Delgadillo JA, Chemineau P, et al. (2017): Computation of a high-resolution MRI 3D stereotaxic atlas of the sheep brain. J Comp Neurol, 525(3), 676-692.
6. Ella A, Keller M (2015): Construction of an MRI 3D high resolution sheep brain template. Magn Reson Imaging, 33(10), 1329-1337.
7. Elmacı C, Şahin Ş, Öner, Y (2013): Distribution of different alleles of aromatase cytochrome P450 (CYP19) and melatonin receptor 1A (MTRN1A) genes among native Turkish sheep breeds. Kafkas Univ Vet Fak Derg, 19(6), 929-933.
8. Ertelt K, Oevermann A, Precht C, et al. (2016): Magnetic resonance imaging findings in small ruminants with brain disease. Vet Radiol Ultrasound, 57(2), 162-169.

9. Evangelisti MA, Varcasia A, Deiana R, et al. (2016): Clinical evolution of cerebral coenurosis from invasive to chronic infection in sheep and a goat. J Infect Dev Ctries, 10(10), 1151-1155.
10. Gonzalo-Orden M, Millán L, Alvarez M, et al. (2003): Diagnostic imaging in sheep hepatic fascioliasis: ultrasound, computer tomography and magnetic resonance findings. Parasitol Res. 90(5), 359-364.
11. Hutchinson EB, Sobakin AS, Meyerand ME, et al. (2013): Diffusion tensor MRI of spinal decompression sickness. Undersea Hyperb Med, 40(1), 23-31.
12. International Committee on Veterinary Gross Anatomical Nomenclature (2017): Nomina Anatomica Veterinaria (N.A.V.). 6th ed., World association of Veterinary Anatomists, Hannover, Gent, Columbia, Rio de Janeiro.
13. Jazini E, Sharan AD, Morse LJ, et al. (2012): Alterations in T2 relaxation magnetic resonance imaging of the ovine intervertebral disc due to nonenzymatic glycation. Spine, 37(4), 209-215.
14. Koff MF, Shah P, Pownder S, et al. (2013): Correlation of meniscal T2* with multiphoton microscopy and change of articular cartilage T2 in an ovine model of meniscal repair. Osteoarthritis Cartilage, 21(8), 1083-1091.
15. König HE, Liebich HG, Cerveny C (2004): Veterinary Anatomy of the Domestic Mammals, Part 14, 465-536. In: HE König and HG Liebich (eds), Nervous System, Schattauer, Stuttgart.
16. Lee W, Lee SD, Park MY, et al. (2015): Functional and diffusion tensor magnetic resonance imaging of the sheep brain. BMC Vet Res, 11, 262-270.
17. Manunta ML, Evangelisti MA, Burrai GP, et al. (2012): Magnetic resonance imaging of the brain and skull of sheep with cerebral coenurosis. Am J Vet Res, 73(12), 1913-1918.
18. Much CC, Schoennagel BP, Yamamura J, et al. (2013): Diffusion-weighted MR imaging of fetal lung maturation in sheep: effect of prenatal cortisone administration on ADC values. Eur Radiol, 23(7), 1766-1772.
19. Nisolle JF, Wang XQ, Squélart M, et al. (2014): Magnetic resonance imaging (MRI) anatomy of the ovine lumbar spine. Anat Histol Embryol, 43(3), 203-209.
20. Skiöld B, Wu Q, Hooper SB, et al. (2014): Early detection of ventilation-induced brain injury using magnetic resonance spectroscopy and diffusion tensor imaging: an in vivo study in preterm lambs. PLoS One. 9(4), e95804.
21. Stypulkowski PH, Stanslaski SR, Jensen RM, et al. (2014): Brain stimulation for epilepsy-local and remote modulation of network excitability. Brain Stimul, 7(3), 350-358.
22. Tkach JA, Hillman NH, Jobe AH, et al. (2012): An MRI system for imaging neonates in the NICU: initial feasibility study. Pediatr Radiol, 42(11), 1347-1356.
23. Valentin S, Licka TF, Elliott J (2015): MRI-determined lumbar muscle morphometry in man and sheep: potential biomechanical implications for ovine model to human spine translation. J Anat, 227(4), 506-513.
24. Vandeweerd JM, Kirschvink N, Muylkens B, et al. (2013): Magnetic resonance imaging (MRI) anatomy of the ovine stifle. Vet Surg, 42(5), 551-558.
25. Wells AJ, Vink R, Blumbergs PC, et al. (2012): A surgical model of permanent and transient middle cerebral artery stroke in the sheep. PLoS One, 7(7), e42157.
26. Wijayathunga VN, Ridgway JP, Ingham E, et al. (2015): Nondestructive Method to Distinguish the Internal Constituent Architecture of the Intervertebral Discs Using 9.4 Tesla Magnetic Resonance Imaging. Spine, 40(24), 1315-1322.
27. Zaky MR, Shaat RM, El-Bassiony SR, et al. (2015): Magnetic resonance imaging (MRI) brain abnormalities of neuropsychiatric systemic lupus erythematosus patients in Mansoura city: Relation to disease activity. The Egyptian Rheumatologist, 37, 7-11.