Arthritisli ishalli buzağılarda eklem sıvısı proteinlerinin SDS-PAGE ile elektroforetik analizi

Abstract

Çalışmada, klinik olarak sağlıklı (kontrol) ve ishalli buzağılara ait arthritisli eklem sıvısı örneklerinde proteinlerin elektroforetik olarak incelenmesi (SDS-PAGE) amaçlanmıştır. Çalışmada 3-29 günlük sağlıklı 7 buzağı (kontrol) ile arthritis gözlenen 19 ishalli buzağıdan (2-75 günlük) materyal olarak yararlanılmıştır. Eklem sıvısı proteinlerinin molekül ağırlıklarına göre ayırımı SDS-PAGE (8x10 cm mini-jelde %10 SDS içeren %7.5 akrilamid) ile gerçekleştirilmiştir. SDS-PAGE sonrası sağlıklı ve arthritisli eklem sıvılarında 48-50 kDa ve 56-57 kDa temel bandlar olarak gözlenmiştir. İki grup arasında fark yaratan 18 protein bandı dikkat çekici bulunmuştur. Sonuç olarak; eklem sıvısında 205.5: 200-190; 144-140; 110-100; 64-62; 39-40 ve muhtemelen ≤10 kDa protein bandlarının tek tek ya da birlikte ishalli buzağılarda arthritisin şiddetini belirleyici bir gösterge olabileceği ve protein bandlarda gözlenen değişikliklerin ishalli buzağılarda eklem bozukluğunun patofizyolojisinde rol oynayan etyolojik faktörleri anlamada yardımcı olabileceği, bu nedenle de Veteriner klinikte tanısal ve prognostik önemi olabileceği kanısına varılmıştır

Keywords

• Arthritis
• buzağı ishali
• eklem sıvısı
• SDS-PAGE
• sinovyal protein

Electrophoretic analysis of arthritic synovial fluid proteins by SDS-PAGE in diarrheic calves

Abstract

In the study, synovial fluid samples obtained from clinically healthy calves (control) and diarrheic calves with arthritis were examined by electrophoresis (SDS-PAGE) to determine the distribution of denatured proteins for understanding their clinical importance. In the study, 3-29 days old 7 healthy calves (control) and 19 diarrheaic calves observed in arthritis (2-75 days old) were used as material. Synovial fluid proteins were separated by SDS-PAGE (7.5% + 10% SDS-containing acrylamide, on 8x10 cm mini-gel) according to the molecular weights. Protein bands with the molecular weight of 48-50 kDa and 56-57 kDa were the main bands after SDS-PAGE analysis of joint fluids of calves with and without arthritis. Eighteen protein bands were dominantly found in arthritic group. As a result, joint fluid protein bands with the molecular weights of 205.5; 200-190; 144-140; 110-100; 6462; 40-39 and <10 kDa may be important together or individually to determine the degree of severity of arthritis in calves. Moreover, these changes in protein bands may also be useful to understand the ethiologic factors in the pathophysiology of arthritis. Therefore, obtained results may have a diagnostic and prognostic value in the veterinary clinics

Keywords

• Arthritis
• calf diarrhea
• SDS-PAGE
• synovial fluid
• synovial protein

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