The first detection and molecular characterization of porcine reproductive and respiratory syndrome virus (PRRSV) in Turkey

Abstract

Porcine Reproductive and Respiratory Syndrome (PRRS) is an important disease that causes severe economic losses in pig industry. PRRSV has two genotypes named as a European (EU) and North American (US). PRRSV appears globally a variety of countries including Canada, USA, South Korea, Germany, Spain, UK, Denmark and Greece. Furthermore, US genotype has been detected serologically in France, Germany and USA in wild boars. So far, no comprehensive information has been generated in Turkey in terms of presence and/or epidemiology of the disease. The aim of this study was to determine presence of PRRSV at initial step and subsequently provide information on possible genotypes that might have occurred in domestic pig and wild boars. The study was carried out on 86 nasal swaps from two different farms and 12 lungs tissue samples from wild boars. A total of 71.4% of samples were found PRRSV- positive by one-step RT-PCR. Prevalence of the virus in Farms 1 and 2 and wild boars was determined as 76.9%, 61.9% and 58%, respectively. Nucleotide sequence analyses performed on ORF 7 of the genome showed that 96.5% and 98.2%, nucleotide homologies in Izmir and Mersin, respectively. In addition, phylogenetic analysis showed that all Turkish PPRSVs was located in the lineage of US like PRRS viruses. This is the first report of PRRSV infection between domestic pigs and wild boars in Turkey

Keywords

• PRRS
• epidemiology
• swine
• Turkey

Türkiye’de domuzlarda porcine reproductive and respiratory syndrome virus (PRRSV)’un ilk tesbitive moleküler karakterizasyonu

Abstract

Porcine Reproductive and Respiratory Syndrome (PRRS) Virus, domuz endüstrisinde önemli ekonomik kayıplara sebep olan bir hastalıktır. Virus, Avrupa ve Kuzey Amerika olmak üzere iki genotipe sahiptir. PRRS virus enfeksiyonu Kanada, Amerika, Kuzey Kore, Almanya, İspanya, İngiltere, Danimarka ve Yunanistan gibi çeşitli ülkelerde görülmektedir. Virusun Kuzey Amerika genotipi Fransa, Almanya ve Amerika’da yaban domuzlarında serolojik olarak tespit edilmiştir. Türkiye’de şimdiye kadar hastalığın varlığına yönelik olarak bir bilgiye ulaşılmamıştır. Bu çalışmada söz konusu enfeksiyonun hem küçük domuz yetiştiriciliklerinde hem de yaban domuzlarında varlığının araştırılması ve sonrasında enfeksiyonu oluşturan virusun genotipine yönelik bilgilerin elde edilmesi amaçlanmıştır. Bu amaçla farklı iki yetiştiricilikten toplam 86 adet nasal swap örneği ile 12 adet yaban domuzundan akciğer doku parçaları alındı. Toplam 98 adet örneğe yapılan one-step RT-PCR testinde % 71.4’lük pozitiflik saptandı. Virusun prevalansı I. yetiştirilicilikte (İzmir) %76.9, II. yetiştirilicikte (Mersin) %61.9 olarak tespit edildi. Yaban domuzlarında ise bu oran %58.3 olarak saptandı. Nükleotid sekans analizi sonucunda genomun ORF 7 bölgesine I.yetiştiricilikte homoloji %96.5, II. yetiştiricilikte ise %98.2 olarak belirlendi. Filogenetik analiz sonucunda çalışmada elde edilen tüm PRRS virusların Amerikan benzeri PRRS virusları içinde yer aldığı tespit edildi. Bu çalışma Türkiye’de evcil ve yaban domuzları arasında PRRSV yönünden yapılan ilk çalışmadır

Keywords

• PRRS
• epidemiyoloji
• domuz
• Türkiye

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