The effect of single epidural plus intramusculer injection of FSH on superovulatory response in Anatolian Black cow

Abstract

The present study was designed to determine whether a single epidural injection plus intramuscular (im) injection of FSH in order to reduce the number of injection treatments required to induce supurovulatory response, as well as to determine the effects of this treatment on Anatolian Black cow (Bos Taurus). The results will be compared to those obtained by administration of twice daily injections of FSH. Twenty-one Anatolian Black cows were used in this study. Anatolian Black cows received Cue-mate containing 1.56 g progesterone. The cows were divided into three groups (D1, D2 and E) based on the dose and administration route of FSH. In group D1 a total of 490 IU FSH and in group D2 a total of 700 IU FSH was given intramuscularly in decreasing doses for twice daily for 4 days. In both groups superovulatory treatments were initiated on day 7 of the cycle. In group E, FSH was given 175 IU epidural plus 175 IU im injection on day 7 of the cycle. On the 9th day of the cycle, the Cue-mate was removed and the cows

Keywords

• Cow
• embryo
• epidural injection
• FSH
• superovulation

İntramuskuler uygulama ile birlikte epidural boşluğa uygulanan FSH’nın Yerli Kara inektesüperovulasyona olan etkisi

Abstract

Bu çalışma süperovulasyon oluşturmak için tekrarlanan dozlarda yapılan FSH uygulama sayısını azaltmak, FSH’nın tek uygulama şeklinde epidural-im olarak uygulanmasının süperovulasyon oluşturma yönünde etkisini belirlemek ve bu uygulamanın Yerli Kara inekte etkisini ortaya koymak amacıyla tasarlandı. Elde edilen sonuçlar günde iki kez azalan dozlarda yapılan FSH uygulama sonuçları ile karşılaştırıldı. Bu çalışmada 21 baş Yerli Kara inek kullanıldı. Yerli Kara ineklere 1.56 g progesteron içeren Cue-mate uygulandı. İnekler FSH uygulama şekli ve dozuna bağlı olarak 3 gruba (D1, D2 ve E) ayrıldı. Grup D1’e toplam 490 IU FSH ve grup D2’ye toplam 700 IU FSH süperovulasyon prokolünün 7. gününde başlamak üzere 4 gün süre ile azalan dozlarda im olarak uygulandı. Grup E’ye ise FSH süperovulasyon prokolünün 7. gününde 175 IU epidural, 175 IU im olmak üzere tek uygulama biçiminde yapıldı. Süperovulasyon protokolünün 9. gününde Cue-mate çıkarıldı ve gruplardaki tüm ineklere 500 µg prostaglandinF2α yapıldı. 11. gün akşam ve 12. gün sabah olmak üzere 12 saat ara ile tüm ineklere Yerli Kara boğalardan alınan spermalar ile suni tohumlama yapıldı. Süperovulasyon uygulanan inekler suni tohumlama uygulamasından 7 gün sonra cerrahi olmayan yöntemle embriyo elde etme çalışmasına alındı. Elde edilen embriyolar Uluslararası Embriyo Transferi Birliğinin bildirdiği kıstaslara göre sınıflandırıldı. Gruplar arasında ortalama CL sayıları yönünden istatistikî bir farklılık gözlenmedi. Ortalama CL sayıları grup D1’de 3.7 ± 0.56; grup D2’de 3.4 ± 0.55 ve grup E’de 6.0 ± 1.14 olarak belirlendi. Transferedilebilir embriyo oranları gruplar arasında farklı değildi (10/42, %23.8 grup D1; 14/32, %43.8 grup D2 ve 19/82, %23.2 grup E). Transferedilemez embriyo oranları da gruplar arasında farklı bulunmadı (32/42, %76.2 grup D1; 18/32, %56.2 grup D2 ve 63/82, %76.8 grup E). Tüm gruplar da UFO oranı benzerdi (17/77, %22.1 grup D1; 13/71, %18.3 grup D2 ve 28/132, %21.2 grup E). Embriyo geri kazanım oranı gruplar arasında farklı bulundu (59/77, %76.6 grup D1; 45/71, %63.4 grup D2 ve 110/132, %83.3 grup E). Sonuç olarak, süperovulasyon oluşturma yönünden FSH’nın tek uygulama şeklinde yapılan epidural-im uygulamasının azalan dozlarda yapılan FSH uygulaması ile karşılaştırıldığında sonuçların azalan dozlarda yapılan uygulamaya yakın olduğu belirlendi. Ancak transferedilebilir embriyo oranı hem epidural-im uygulamada hem de azalan dozlarda yapılan uygulamalarda düşük bulundu. Transferedilebilir embriyo oranını artırmak için farklı ırklarda daha ileri çalışmaların yapılması gerektiği benimsendi

Keywords

• Embriyo
• epidural enjeksiyon
• FSH
• inek
• süperovulasyon

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