Investigation of extended-spectrum beta-laktamase activity and antibiotic resistance profile ofEscherichia coli strains isolated from bovine mastitis

Abstract

The aim of this study was to investigate the extended-spectrum beta-laktamase activity (ESBL) and antibiotic resistance profile of 92 Escherichia coli strains isolated from bovine mastitic milk samples. Milk samples were obtained from dairy farms in Ankara, Balıkesir and Çorum. ESBL screening test, ESBL phenotypic confirmation test and in vitro susceptibility test for twelve antibiotics were performed with Kirby Bauer disc diffusion method in E. coli strains. Among the E. coli strains, respectively the highest resistance rates were detected against to erythromycin (69.6%), ampicillin (39.1%), tetracycline (34.8%), nalidixic acid (25.0%), chloramphenicol (22.8%), trimethoprim-sulfamethoxazole and amoxicillin-clavulanic acid (21.7%). Of all strains, 25.0% were found susceptible to all antibiotics and %54.3 of E. coli were resistant to two or more antibiotics.This was the first study that ESBL activity of E. coli isolated from mastitis was investigated in our country. However among the E. coli isolates, ESBL activity was not detected. This study showed that high rates of multidrug resistance developed in E. coli strains caused mastitis

Keywords

• Antibiotic resistance
• beta-laktamase
• E. coli
• mastitis

Sığır mastitislerinden izole edilen Escherichia coli suşlarında genişlemiş spektrumlu beta-laktamaz aktivitesi ve antibiyotik dirençlilik profilinin incelenmesi

Abstract

Bu çalışmada Ankara, Balıkesir ve Çorum’daki süt işletmelerinden sağlanan mastitis şüpheli sütlerden izole edilen 92 adet E. coli suşunda genişlemiş spektrumlu beta-laktamaz (GSBL) aktivitesi ve antibiyotik direnç profilinin araştırılması amaçlandı. E. coli suşlarında Kirby Bauer disk diffüzyon yöntemi ile GSBL tarama testi, fenotipik GSBL doğrulama testi ve 12 adet antibiyotik için in vitro duyarlılık testleri yapıldı. İncelenen E. coli suşlarında en yüksek dirençlilik oranları sırasıyla eritromisine (%69.6), ampisiline (%39.1), tetrasikline (%34.8), nalidiksik aside (%25.0), kloramfenikole (%22.8), trimetoprim-sülfametaksazole (%21.7) ve amoksisilin klavulonik aside (%21.7) karşı bulunurken, suşların %25.0’inin kullanılan tüm antibiyotiklere duyarlı olduğu saptandı. Ayrıca E. coli suşlarının %54.3’ünün iki veya daha fazla sayıda antibiyotiğe dirençli olduğu belirlendi. Bu çalışma ile ülkemizde mastitis orijinli E. coli suşlarında ilk kez GSBL aktivitesi araştırıldı. Ancak, incelenen E. coli suşlarında GSBL aktivitesi saptanmadı. Çalışmada elde edilen bulgular mastitise neden olan E. coli suşlarında yüksek oranda çoğul ilaç direncinin geliştiğini gösterdi

Keywords

• Antibiyotik dirençlilik
• E. coli
• GSBL
• mastitis

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