Determination of SCCmec types in methicillin resistant staphylococci isolated from cows and farm workers

Abstract

This study was conducted to determine staphylococcal cassette chromosome mec (SCCmec) types of methicillin resistant staphylococci (MRS) isolated from cattle and farm workers. A total of 145 subclinic mastitic bovine milk and 91 nasal swab samples (56 from cows and 35 from farm workers) were studied for presence of MRS. Methicillin resistance was detected by cefoxitin disc diffusion test. Bacterial identification and methicillin resistance was confirmed by 16S rRNA sequencing and mecA PCR, respectively. SCCmec types of these isolates were determined by multiplex PCR targeting for mec and ccr genes, associated IS element and plasmids. Among 236 total samples, 181 staphylococci, 59 of which were found as methicillin resistant were isolated. Fifty nine methicillin resistant isolates originated from 37 (62.7%) were bovine and 22 (37.3%) were human. Ten Staphylococcus species [S. haemolyticus (18), S. epidermidis (17), S. aureus (12), S. xylosus (4), S. cohnii (3), S. equorum (1), S. sciuri (1), S. succinus (1), S. pseudointermedius (1) and S. saprophyticus (1)] among 59 MRS were identified by 16S rRNA sequence analysis. SCCmec types of 2, 38, 18, and 1 MRS isolates were II, III, IV, and V, respectively. A total of 40 isolates including type II and III were hospital acquired whereas 19 isolates including type IV and V were community acquired. Almost all S. haemolyticus and S. aureus isolates carried hospital acquired type SCCmec. The similarity of SCCmec types between human and cattle isolates suggest evidence of transmission from animals to humans, or vice versa. Further studies are needed to establish clonal relationship of MRS isolated from bovine and farm workers with advanced molecular techniques

Keywords

• Staphylococcus
• methicillin
• SCCmec
• cattle
• human

Sığırlardan ve sektör çalışanlarından izole edilen metisilin dirençli stafilokoklarda SCCmec tiplerininbelirlenmesi

Abstract

Bu çalışma sığır ve çiftlik çalışanlarından izole edilen metisilin dirençli stafilokokların (MRS) stafilokokal kaset kromozom mec (SCCmec) tiplerini belirlemek amacıyla gerçekleştirildi. Toplam 145 subklinik mastitisli inek sütü ve 91 burun sıvap örneği (56 inekten ve 35 çiftlik çalışanından) MRS varlığı yönünden incelendi. Metisilin direnci sefoksitin disk difüzyon yöntemi ile belirlendi. Bakteriyel identifikasyon ve metisilin direnci sırasıyla 16S rRNA sekansı ve mecA PCR ile doğrulandı. Bu izolatların SCCmec tipleri mec ve ccr, IS elementi ve plazmidler için multipleks PCR ile incelendi. Bu 236 örnekten 181 stafilokok izole edilirken bunların 59’u metisilin dirençli olarak belirlendi. Elli dokuz izolatın 37 (%62.7)’si sığır ve 22 (%37.3)’si insan orjinliydi. 16S rRNA sekans analizi ile incelenen 59 MRS içinde 10 tür [S. haemolyticus (18), S. epidermidis (17), S. aureus (12), S. xylosus (4), S. cohnii (3), S. equorum (1), S. sciuri (1), S. succinus (1), S. pseudointermedius (1), S. saprophyticus (1)] identifiye edildi. SCCmec tip II, III, IV ve V sırasıyla 2, 38, 18 ve 1 MRS izolatında belirlendi. SCCmec Tip II ve III’ü taşıyan 40 izolat hastane kökenli, SCCmec Tip IV ve V’i içeren 19 izolat toplum kökenli olarak tespit edildi. Hemen tüm S. haemolyticus ve S. aureus izolatlarının hastane kökenli SCCmec tiplerini taşıdığı belirlendi. Sığır ve insan stafilokok izolatlarının taşıdığı SCCmec tipleri arasındaki benzerlik hayvanlardan insanlara ya da tam tersi bir bulaşma olabileceğini düşündürmektedir. Sığır ve çiftlik çalışanlarından izole edilen MRS’lar arasındaki klonal ilişkinin belirlenebilmesi için ileri moleküler teknikler kullanılarak gerçekleştirilecek olan çalışmaların yapılmasına ihtiyaç duyulmaktadır

Keywords

• Staphylococcus
• metisilin
• SCCmec
• sığır
• insan

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