Investigation of the presence and antibiotic susceptibilities of Flavobacterium psychrophilum in rainbow trout farms (Oncorhynchus mykiss Walbaum, 1792) in The Middle and Eastern Black Sea Regions of Turkey

Abstract

The aims of the study were to find out the presence of Flavobacterium psychrophilum (F. psychrophilum) in commercial Rainbow trout farms in Middle and Eastern Black Sea regions and to determine the antibiotic susceptibility profiles of the isolated strains. For this purpose, total of 276 fish samples were collected from 18 different farms in these regions between January 2008 and July 2010. Bacterial isolation from samples was performed by conventional microbiological methods. In addition, Polymerase Chain Reaction (PCR) was used to confirm the identification and to detect F. psychrophilum in tissue samples. Nine of fish tissue samples were found to be positive for F. psychrophilum by PCR. However, five F. psychrophilum strains were isolated from these samples and confirmed by PCR. The antibiotic resistance of the isolates against neomycine, oxytetracycline, enrofloxacin, amoxycillin, ampicillin, erythromycin, kanamycin, sulphamethoxazole+trimethoprim, cefoperazone and oxolinic acid were analyzed by Kirby Bauer agar disc diffusion test. The antibiotic susceptibility patterns of the isolates were variable. All the isolates were sensitive only to oxytetracyline (30 µg) and enrofloxacine (5 µg)

Keywords

• Antibiotic susceptibility
• F. pscychrophilum
• identification
• PCR

Orta ve Doğu Karadeniz Bölgesi gökkuşağı alabalığı çiftliklerinde (Oncorhynchus Mykiss Walbaum,1792) Flavobacterıum psychrophilum varlığının ve antibiyotik duyarlılıklarınının incelenmesi

Abstract

Bu çalışma, Orta ve Doğu Karadeniz bölgesinde bulunan ticari gökkuşağı alabalığı çiftliklerinde F. psychrophilum’un varlığının ve antibiyotik duyarlılıklarının belirlenmesi amacıyla yapılmıştır. Çalışma kapsamında Ocak 2008-Temmuz 2010 tarihleri arasında bölgedeki 18 farklı çiftlikten toplam 276 balık örneği toplandı. Örneklerden bakteri izolasyonu konvansiyonel mikrobiyolojik metotlar ile yapıldı. Elde edilen izolatların moleküler olarak doğrulanmasında ve doku örneklerinden etkenin saptanmasında Polimeraz Zincir Reaksiyonu (PZR) kullanıldı. Balık dokularının dokuzunun PZR ile pozitif sonuç verdiği saptandı. Bu dokuların beşinden F. psychrophilum izole edildi ve PZR ile doğrulandı. İzolatların neomisin, oksitetrasiklin, enrofloksasin, amoksilin, ampisilin, eritromisin, kanamisin, sulfametoksazol+trimetoprim, sefoperazon ve oksolinik asit antibiyotiklerine karşı direnç durumu, Kirby Bauer agar disk diffüzyon yöntemi ile araştırıldı. Antibiyotik duyarlılık profilinin değişken olduğu görüldü. İzolatların tamamının oksitetrasiklin ve enrofloksasin antibiyotiklerine karşı duyarlı olduğu belirlendi

Keywords

• Antibiyotik duyarlılığı
• F. pscychrophilum
• identifikasyon
• PCR

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