Korunga (Onobrychis Viciifolia) bitkisinin antioksidan etkinliğinin in vitro değerlendirilmesi

Abstract

Bu çalışmada, korunga bitkisinin (Onobrychis viciifolia) toprak üstü kısımlarının aseton, methanol, etilasetat ve su ekstrelerinin antioksidan etkinliği araştırıldı. Ekstrelerin fenolik içeriği Folin-Ciocalteu yöntemiyle, antioksidan aktivitesinin belirlenmesi fosfomolibden metodu, beta-karoten-linoleat model sistemi, radikal süpürücü etkiye karşı 2,2-difenil-1-pikrilhidrazil (DPPH) ve demirli kompleks olan Fe+3/K3Fe(CN)6 bileşiğinin indirgenmesi ile gerçekleştirildi. Bu çalışma, yüksek antioksidan özelliği gösteren korunga bitkisinin aseton ve metanollü ekstrelerinin, ileride farmasötik çalışmalar için değerlendirilebileceğini önermektedir

Keywords

• Antioksidan aktivite
• serbest radikal süpürücü aktivite
• O. Viciifolia

Determination of in vitro antioxidant activity of the sainfoin (Onobrychis Viciifolia) extracts

Abstract

In this study, in vitro antioxidant effects of the acetone, methanol, ethylacetate and water extracts of the aerial parts of sainfoin (Onobrychis viciifolia) were investigated. The phenolic content of the extracts were determined by Folin-ciocalteu method and determination of antioxidant activity were carried out by phosphomolybdenum method, β-carotene-linoleate model system, radical scavenging capacity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power based on the reduction of Fe+3/K3Fe(CN)6 complex to the ferrous state. The study suggested that sainfoin could be pharmaceutically exploited in future researches for its antioxidant properties where acetone and methanol extracts exhibited high antioxidant activity

Keywords

• Antioxidant activity
• free radical scavenging activity
• O. viciifolia

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