Mycoplasma gallisepticum ile doğal enfekte kınalı kekliklerde (Alectoris chukar) ve Japon bıldırcınlarında (Coturnix coturnix japonica) patomorfolojik ve immünohistokimyasal incelemeler

Abstract

Çalışmada, doğal enfekte kınalı kekliklerde (5 adet) ve Japon bıldırcınlarında (11 adet) Mycoplasma gallisepticum tarafından oluşturulan mikoplazmozisin patomorfolojik bulguları ve immünohistokimyasal olarak da mikroorganizma antijenlerinin doku yerleşimleri tanımlandı. Her iki hayvan türünde; durgunluk, yem tüketiminde azalma, zayıflama, serözden katarala kadar değişen nazal akıntı ve konjuktivitis dikkati çeken klinik bulgulardı. Bu bulguları takiben hayvanların genellikle 3-4 hafta içinde öldüğü belirtildi. Mortalitenin kınalı kekliklerde % 43, Japon bıldırcınlarında % 37 olduğu bildirildi. Nekropside en çok göze çarpan bulgu; nazal ve paranazal sinüslerde, tek ya da çift taraflı, değişen derecelerde şişkinlik ve içleri sarımtrak renkte kazeöz eksudat birikimiydi. Bazı olgularda hava keseleri kalınlaşmış ve mat bir görünüme sahipti. Histopatolojik incelemede; sinüsler genellikle nekrotik doku kalıntıları, dökülmüş epitel hücreleri, bakteri kümeleri ve heterofil lökositlerden oluşan kazeifiye eksudat içermekte idi. Ayrıca, sinüslerde müköz bezlerde hiperplazi, submukozada fokal lenfoid hücre hiperplazileri ve/veya diffuz mononüklear hücre infiltrasyonları göze çarptı. Tavuktan elde anti-Mycoplasma gallisepticum poliklonal antikoru kullanılarak yapılan immünofloresan incelemede, bakteriyel antijenler nazal ve paranazal sinüslerdeki eksudat içerisinde yoğun şekilde; daha az olmak üzere de trake, hava keseleri, akciğerler ve bağırsaklarda saptandı. Çalışma sonuçları, Mycoplasma gallisepticum’un izolasyonunun ve identifikasyonunun gerçekleştirilemediği koşullarda, immünofloresan yöntemin hızlı ve güvenilir bir şekilde kullanılabileceğini ortaya koymuştur

Keywords

• İmmünofloresan
• Japon bıldırcını (Coturnix coturnix japonica)
• kınalı keklik (Alectoris chukar)
• Mycoplasma gallisepticum

Pathomorphological and immunohistochemical examinations in naturally infected partridges(Alectoris chukar) and Japanese quails (Coturnix coturnix japonica) with Mycoplasma gallisepticum

Abstract

The present study describes pathomorphological findings and tissue localisation of bacterial antigens immunohistochemically in naturally infected partridges (n: 5) and Japanese quails (n: 11) with Mycoplasma gallisepticum. In both animal species, marked clinical findings were lethargy, decreased food consumption and body condition, conjunctivitis as well as nasal discharge ranging from serous to catarrhal. Following these findings, death of the animals occurred usually within 3-4 weeks. The mortality rate was 43% and 37% in partridges and Japanese quails, respectively. At necropsy, nasal and paranasal sinuses were filled with yellowish caseous exudate uni- or bilaterally at varying degrees. In some cases, air sacs were thickened and opaque in appearance. In histopathological examination, sinuses were filled with caseous exudate including necrotic tissue debris, clumps of bacteria, and heterophile leukocytes. Additionally, there was hyperplasia of mucous glands and focal lymphoid cell hyperplasia and/or diffuse mononuclear cell infiltrations in submucosa of sinuses. In fluorescent antibody examination using polyclonal chicken anti-Mycoplasma gallisepticum primary antibody, bacterial antigens were found abundantly in the exudate in nasal and paranasal sinuses and, to a lesser extend, in trachea, air sacs, lungs and intestines. As a result, this study indicated that if Mycoplasma gallisepticum couldn’t be isolated and identificated, immunofluorescence method could be used quickly and safely

Keywords

• Immunofluorescence
• japanese quails (Coturnix coturnix japonica) Mycoplasma gallisepticum
• partridges (Alectoris chukar)

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