Virulence genes of Shiga toxin-producing Escherichia coli O157:H7 strains isolated from calves and cattle

Abstract

The aim of this study was to detect E. coli O157:H7 serotype in the faeces samples collected from calves and cattle farms located of Afyonkarahisar province in Turkey and to determine the stx(1) (Shiga toxin 1) and stx(2) (Shiga toxin 2) virulence genes in the strains of E.coli O157:H7 by multiplex PCR (mPCR). In this study, E. coli O157:H7 was isolated from the 3.1% (14 out 457 faeces samples) of the calves and cattle examined, in particular 2.3% of the cattle, 2.6% of non-diarrhoeic calves and 10.6% of diarrhoeic calves were positive for E. coli O157:H7. The stx(1) and stx(2) genes were detected in 6 out of 14 (42.8%) DNA samples extracted from STEC O157:H7 strains. This study demonstrated of E. coli O157:H7 serotype in cattle and calves, which represent an important reservoir for strains that a potential risk for human infections

Keywords

• Calves
• cattle
• Escherichia coli O157:H7
• mPCR
• stx(1) and stx(2) genes

İnek ve buzağılardan izole edilen Şiga toksijenik Escherichia coli O157:H7 suşlarının bazı virülensgenleri

Abstract

Bu çalışmanın amacı, Afyonkarahisar’da bulunan çifliklerdeki inek ve buzağılardan toplanan dışkı örneklerinden E. coli O157:H7 serotiplerini izole etmek, stx(1) (Şiga toksin 1) ve stx(2) (Şiga toksin 2) virülens genlerini multipleks PCR (mPCR) ile belirlemektir. Bu çalışmada, inek ve buzağılardan alınan toplam 457 dışkı örneğinin 14 (%3.1)’ünden E. coli O157:H7 serotipi izole edildi. İzole edilen suşların %2.3’ü ineklerden, %2.6’sı sağlıklı buzağılardan, %10.6’sı ise ishalli buzağılardan elde edildi. Şigatoksijenik E. coli (STEC) O157:H7 suşlarından elde edilen 14 DNA örneğinin 6 (%42.8)’sında stx(1) ve stx(2) geni pozitif bulundu. Bu çalışma, inek ve buzağılardan izole edilen STEC O157:H7 serotiplerinin insan enfeksiyonları için önemli bir risk faktörü olabileceğini göstermektedir

Keywords

• Buzağı
• Escherichia coli O157:H7
• inek
• mPCR
• stx(1) ve stx(2) genleri

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