Transcriptional Regulation of stj Fimbrial Operon in Salmonella Typhimurium

Abstract

The stj fimbrial operon harbors five genes, stjABCDE. The operon plays role in the persistence and virulence of S. Typhimurium in host systems. In this study, in vitro activation of the stj operon was achieved by transposon (TPOP) mutagenesis. The transposon insertion site was located within the cadC gene promoter in the stj operon-activated mutant strain MDN20. Comparative analysis of two-dimensional gel electrophoresis protein patterns of the wild type strain (S. Typhimurium LT2) and mutant strain MDN20 revealed that the CadC and StjB proteins were produced by mutant strain MDN20 but not by the wild type strain. These results were also confirmed by Maldi-TOF analysis. These findings indicate that cadC gene is a transcriptional activator of stj fimbrial operon in S. Typhimurium

Keywords

• S. Typhimurium
• stj operon
• cadC
• transcriptional activator

Salmonella Typhimurium’da stj fimbriyal operonunun transkripsiyonel regülasyonu.

Abstract

Salmonella Typhimurium’da konakçı sistemde kalıcılığı ve virulensliğinde rol oynayan stj fimbriyal operonu, stjABCDE olmak üzere beş gen içermektedir. Bu çalışmada, stj fimbriyal operonunun in vitro koşullarda aktivasyonu transpozon (TPOP) mutasyonu yolu ile gerçekleştirildi. stj operonu aktive edilen mutant suşta (MDN20), traspozonun cadC geni promotoruna girdiği belirlendi. Doğal suş (S. Typhimurium LT2) ve MDN20 mutant suşunda gerçekleştirilen iki yönlü jel elektroforezi çalışmaları sonucunda; CadC ve StjB proteinlerinin doğal suşta üretilmediği, ancak MDN20 mutant suşunda üretildiği saptandı. Bu sonuçlar ayrıca Maldi-TOF analizleri ile de doğrulandı. Bu bulgular, S. Typhimurium’da cadC geninin, stj operonunun transkripsiyonel aktivatörü olduğunu gösterdi

Keywords

• S. Typhimurium
• stj operonu
• cadC
• transkripsiyonel aktivatör

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