Clinical, pathological and immunohistochemical findings of bovine cutaneous papillomatosis

Abstract

Bovine cutaneous papillomatosis caused by a bovine papillomavirus, is a common skin disease in Turkey. The aim of the present study was to describe the clinical, histopathological and immunohistochemical aspects of naturally occurring bovine cutaneous papillomatosis.A total of 82 Holstein cattle (9.5%), aged between 5 and 24 months, were diagnosed as cutaneous papilloma by clinical examination. The percentage of papilloma and papillomatosis in male and female was found in 7.3% and 14.8%, respectively. The cauliflower-like growths of varying sizes (0.5-11 cm) were mostly located on the head (63.2%). Histopathology revealed various degrees of acanthosis and hyperkeratosis in all neoplasms. Immunohistochemical (IHC) examination with antibodies against proliferating cell nuclear antigen (PCNA) and Ki-67 were detected in the basal layer of the epidermis and connective tissue. Bovine papillomavirus (BPV-1) antigens were detected in the basal layer. In conclusion, it was decided that the BPV-1, PCNA and Ki-67 antibodies were very useful markers in the diagnosis of bovine cutaneous papilloma

Keywords

• Bovine papillomatosis
• BPV-1
• Ki-67
• PCNA

Sığırlarda deri papillomunda klinik, patolojik ve immunohistokimyasal bulgular

Abstract

Bovine papillomavirusun neden olduğu deri papillomatozisi ülkemizde sığırların yaygın bir deri hastalığıdır. Bu çalışmanın amacı doğal olarak oluşmuş sığır deri papillomatozisinde klinik, histopatolojik ve immunohistokimyasal bulguların değerlendirilmesidir. 5 ile 24 ay arasında değişen 82 adet Holstein sığıra (% 9,5) klinik olarak deri papillomu tanısı konuldu. Papilloma ve papillomatozisin erkek ve dişi hayvanlar arasında görülme yüzde oranı sırasıyla %7,3 ve %14,8 olarak belirlendi. Çeşitli boyutlarda (0,5–11 cm) karnabahar görünümündeki üremelerin çoğunlukla baş bölgesinde (%63,2) yerleşim gösterdiği belirlendi. Tümörlerde mikroskobik olarak değişen derecelerde akantozis ve hiperkeratoz saptandı. İmmunohistokimyasal boyamalarda epidermisin bazal katmanında ve bağ dokuda PCNA ve Ki-67 pozitif reaksiyonlar tespit edildi. Bovine papillomavirus antijenleri bazal katmanda belirlendi. Sonuç olarak BPV-1, PCNA ve Ki-67 primer antikorlarının sığır deri papillomunun tanısında önemli markırlar olduğuna karar verildi

Keywords

• Sığır papillomatozis
• BPV-1
• Ki-67
• PCNA

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