Evaluation of nitric oxide level in prepartum heifer mastitis

Abstract

This study was carried out for detection of nitric oxide (NO) levels in healthy (Group 1, n:78) and infected (Group 2, n:63) quarters of pregnant heifers. The mean nitric oxide (NO) concentrations were calculated of about 15,58 ± 2,21 µM/ml and 42,69 ± 6,03 µM/ml for Group 1 and Group 2, respectively. A significant difference was found between two groups, statistically (p<0,001). The mean NO concentrations were compared between quarters infected with Staphylococcus aureus (S. aureus) and coagulase negative staphylococcus (CNS) in Group 2 and measured to be; 38,50 ± 8,47 µM/ml and44,17 ± 7,71µM/ml, respectively (p>0,05). Additionally, lacteal secretions were classified to be thickened and watery according to viscosity. Intramammarial infection rates (IMI) and NO concentrations were compared in low (watery) and high (thickened) viscosity samples. Risk of IMI was found higher in thickened samples than watery (p<0,001). Every 1 unit (µM/ml) increase in the NO concentration boosted the risk of IMI 1,036-fold (p<0,001)

Keywords

• Coagulase negative staphylococcus
• heifer mastitis
• nitric oxide
• Staphylococcus aureus

Prepartum dönemde mastitis şekillenen düvelerde nitrik oksit düzeylerinin değerlendirilmesi

Abstract

Bu çalışma gebe düvelerin sağlıklı (Grup 1, n:78) ve enfekte (Grup 2, n:63) meme loblarında nitrik oksit düzeylerini belirlemek için planlandı. Grup 1 ve Grup 2 deki ortalama nitrik oksit düzeyleri sırasıyla 15,58 ± 2,21 µM/ml ve 42,69 ± 6,03 µM/ml olarak belirlendi ve iki grup arasında NO düzeyi bakımından istatistiki olarak belirgin bir fark bulundu (p<0,001). Grup 2’de Staphylococcus aureus ve koagulaz negatif stafilokoklar ile enfekte meme loblarındaki ortalama NO düzeyleri kıyaslandı ve sırasıyla; 38,50 ± 8,47 µM/ml ve44,17 ± 7,71µM/ml olarak ölçüldü (p>0,05). Nitrik oksit düzeyine ek olarak lakteal sekresyonları viskozitesine göre yoğun ve sulu olarak sınıflandırıldı. Düşük (sulu) ve yüksek (yoğun) viskoziteli örneklerde meme içi enfeksiyon oranları ve NO konsantrasyonları kıyaslandı. Yoğun örneklerde meme içi enfeksiyon riski, sulu örneklerden daha yüksek bulundu (p<0,001). Nitrik oksit konsantrasyonundaki bir birimlik artışın, enfeksiyon riskini 1,036 kat artırdığı belirlendi (p<0,001)

Keywords

• Koagulaz negatif stafilokoklar
• düve mastitisi
• nitrik oksit
• Staphylococcus aureus

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