Evaluation of erythrocyte and platelet indices in canine visceral leishmaniasis

Abstract

The purpose of the study was to evaluate erythrocyte indices and platelet indices in dogs with leishmaniasis (CL, L. infantum). CL was diagnosed by polymerase chain reaction, and confirmed by western blott in 54 dogs. These dogs were divided into three groups; asymptomatic (n=16), oligosymptomatic (n=16), and symptomatic (n=22). Leishmaniasis free 25 dogs were served as controls. Microcytic or normocytic-hypochromic anemia was observed in dogs with CL. There was not a significant difference on RDW (RBC distribution width) groups. PLT count was slightly lower in dogs with CL in all clinical stages (p<0.05), compared to controls. MPV (mean platelet volume) in dogs with CL was higher (P<0.05) compared with controls, and it was significantly higher in asymptomatic dogs. RBC (red blood cell) and platelet indices may be useful parameters during course of the leismaniasis. These indices may have diagnostic properties but are not enough to differentiate of the clinical stages of CL. Further comprehensive studies are needed to clinical use of RBC and platelets indices in canine practice

Keywords

• dog
• erythrocyte
• indices
• leishmaniasis
• platelet

Canine visceral leishmaniasisli köpeklerde eritrosit ve trombosit indekslerinin değerlendirilmesi

Abstract

Bu çalışmanın amacı, Canine Leismaniazis (CL, L. infantum) tanısı konan köpeklerde eritrosit ve platelet indekslerindeki değişimleri değerlendirmektir. Bu çalışmanın materyalini, farklı ırk, yaş ve cinsiyetteki 54 köpek oluşturdu. CL (L.infantum) tanısı, PCR (poymerase chain reaction) yöntemiyle kondu, Western blott yöntemiyle de teyit edildi. Çalışmayı oluşturan köpekler üç gruba ayrıldı: Bu gruplar, asemptomatik (n:16), oligosemptomatik (n:16) ve semptomatik (n:22) köpeklerin (en az üç karakteristik bulguyu taşıyan köpekler) oluşturduğu gruplar olarak düzenlendi. Ayrıca 25 adet sağlıklı köpek kontrol grubunu oluşturdu. CL’lı köpeklerde eritrositlerin morfolojik değerlendirmesinde mikrositik, normositik ve hipokromik oldukları saptandı. Gruplar arasında RDW değeri açısından bir fark saptanmadı. PLT değerinin bütün klinik safhalarda hafif düşük (p<0.05) olduğu belirlendi. MPV değeri CL köpeklerde sağlıklı köpeklere göre (p<0.05) önemli derecede yüksekti. Ayrıca diğer gruplara göre özellikle asemptomatik grupta daha yüksek olduğu saptandı. Bu çalışmada, eritrosit ve platelet indekslerinin CL’nın seyri esnasında kullanışlı parametreler olarak değerlendirilebileceği, klinik safhaların ayrımında kullanışlı olmamakla birlikte diagnostik uygunluğa sahip olduğu kanısına varıldı. İleride klinik pratikte, eritrosit ve platelet indekslerinin klinik kullanımına ilişkin daha ayrıntılı çalışmalara ihtiyaç duyulmaktadır

Keywords

• Eritrosit
• indeks
• köpek
• leismaniazis
• platelet

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