Light and electron microscopic studies on the structure of the male reproductive system in quail(Coturnix coturnix japonica)

Abstract

The aim of this study was to investigate the histological structure of the testis, epididymis and ductus deferens of quail male reproductive system by light and electron microscopy. In this study, tissue samples taken from 10 of the healthy and mature male quails were used as material. Relatively low number of Leydig cells and thin connective tissue trabecules were seen in the intertubular spaces. Seminiferous tubuli directly opened to rete testis (RT). Ductuli efferentes (DE) were investigated into two separate parts, as proximal (DE-P) and distal (DE-D). RT directly connected to DE-P. opened into connecting ducts those finally formed unique ductus epidymidis of each testis. Next to ductus epididymidis and located out of epididymis, ductus deferens opened into cloaka after contortion. In electron microscopic investigation, Leydig cells were seen interstitium and two types of the cells were distinguished. One type had many secretory granules and mitochondri while the 2 nd type contained less numerous mitochondria and electron-lucent vacuoles. DE’s epithelium was formed by two types of cells, ciliated and non-ciliated. Non-ciliated cells located in DE-P had empty centred lipid vacuole and large, dense granules. Non-ciliated epithelial cells of DE-D did not contain lipid vacuoles. Apocrine type secretion was observed in at the epithelium of ductus epididymidis. Apocrine type secretion, was peculiar to the caudal ductus deferens, where as it was not seen in the cranial part. As a result, histology of RT, DE, CD, ductus epididymidis and ductus deferens of quail male reproductive system was introduced by light and electron microscopy

Keywords

• Quail
• Epididymis
• Ductus deferens
• Light-electron microscopy
• Testis

Bıldırcın (Coturnix coturnix japonica) erkek genital sisteminin yapısı üzerinde ışık ve elektron mikroskobik çalışmalar

Abstract

Bu araştırmada, bıldırcın erkek genital sistem organlarından testis, epididimis ve duktus deferensin histolojik yapısı ışık ve elektron mikroskobik olarak incelendi. Çalışmada materyal olarak ergin ve sağlıklı 10 adet erkek bıldırcının genital sisteminden alınan doku örnekleri kullanıldı. İntertubuler alanlarda bağ doku ve Leydig hücrelerinin çok az olduğu saptandı. Seminifer tubullerin doğrudan rete testise (RT) açıldığı görüldü. Duktuli efferentesler (DE), proksimal (DE-P) ve distal (DE-D) olmak üzere iki bölge halinde incelendi. Rete testisin doğrudan DE-P’ye bağlandığı tespit edildi. DE-D’lerin açıldığı bağlayıcı kanalların (CD) birleşerek her testisin duktus epididimidisini oluşturduğu gözlendi. Elektron mikroskobik incelemelerde seminifer tubullerin arasında bulunan Leydig hücrelerinin iki tip olduğu görüldü. Bunlardan birinde bol miktarda salgı vezikülü ve mitokondriyon görülürken, diğerinde az miktarda mitokondriyon ve içi boş vakuoller olduğu belirlendi. DE epitelinin silyumlu ve silyumsuz olmak üzere iki tip hücreden oluştuğu saptandı. DE-P’lerde yer alan silyumsuz hücrelerde merkezleri boş yağ vakuolleri ve koyu içerikli iri granüller saptandı. DE-D’lerin silyumsuz epitel hücrelerinde yağ vakuolleri gözlenmedi. Duktus epididimidis epitelinde apokrin türde salgıya rastlandı. Kaudal duktus deferens epitelinde yoğun, apokrin türde salgıya rastlanırken, kraniyelde bu tip bir salgılama görülmedi. Sonuç olarak, bıldırcın erkek genital sistem organları olan testis, RT, DE, CD, duktus epididimidis ve duktus deferensin histolojik yapısı ışık ve elektron mikroskobik seviyede ortaya kondu

Keywords

• Bıldırcın
• Epididimis
• Duktus deferens
• Işık-Elektron mikroskop
• Testis

References

1. Aire, TA (1980): The Ductuli Efferentes of the Epididymal Region of Birds. J. Anat., 130: 707-723
2. Aire, TA (1997): The Structural of the Interstitial Tissue of the Active and Resting Avian Testis. Onderstepoort J. Vet. Res., 64: 291-299
3. Aire, TA (2000a): Aspects of the Functional Morphology of the Ductus Epididymidis in Domestic Anseriform and Galliform Bird. Anat. Histol. Embryol., 29: 179-191
4. Aire, TA (2002a): Cyclical Reproductive Changes in the Non-ciliated Epithelia of the Epididymis of Birds. Anat. Histol. Embryol., 31: 113-118
5. Aire TA, Ayeni JS, Olowo-Okurun MO (1979): The Structure of the Excurrent Ducts of the Testis of the Guinea-fowl (Numida meleagris). J. Anat., 129: 633-643
6. Aire TA, Soley JT (2000): The Surface Features of the Epithelial Lining of the Ducts of the Epididymis of the Ostrich. Anat. Histol. Embryol., 29: 119-126
7. Aire TA, Soley, JT (2003): The Morphological Features of the Rete Testis of the Ostrich. Anat. Histol. Embryol., 207: 355-361
8. Bacha WJJr, Wood LM (1990): Color Atlas of Veterinary Histology. London: Lea&Febiger. p.: 189-206

9. Bakst MR, Akuffo V, Trefil P, Brillard JP (2007): Morphological and Histochemical Characterization of the Seminiferous Epithelial and Leydig Cells of the Turkey. Anim. Reprod. Sci., 97: 303-313
10. Bancroft JD, Gamble M. (2002): Histological Tecniques. Elsevier Science Limited. Edinburg London New York. 5nd Ed.
11. Bellamy SJ, Kendall MD (1985): The Ultrastructure of the Epithelium of the Ductuli Efferentes Testis in the Common Starling (Sturnus vulgaris). J. Anat., 140: 189- 203
12. Bergmann M, Schindelmeiser J (1987): Development of the Blood-Testis Barrier in the Domestic Fowl (Gallus domesticus). Int. J. Androl., 10: 481-488
13. Cooksey EJ, Rothwell B. (1973): The Structure of the Sertoli Cell and Its Differentiation in the Domestic Fowl (Gallus domesticus). J. Anat., 114: 329-345
14. Cullıng CFA, Allison RT, Barr WT (1985): Cellular Pathology Technıque. Typeset by Mid-County Pres. London. 4nd Ed.
15. Denk H, Kunzele H, Plenk H., Ruschoff J, Sellner W (1989): Romeis Microscopische Tecnic. 17., Neubearbeitete Auflage. Urban und Schwarzenberg, München, Wien, Baltimore. p.: 439-450
16. Dursun N (2004): Evcil Kuşların Anatomisi. Medisan Yayın Serisi. Türkiye. p: 106- 115
17. Düzügan M (2006): Distribution of Acid Glycosidases in the Male Genital Tract of the Pheasant. Repro. Biol. 6 Suppl., 2: 65-73
18. Hayat MA (1981): Fixation For Electron Microscopy. London: Academic Pres. p.: 252-255
19. Hess RA, Thurston RJ, Biellier HV (1976): Morphology of the Epididymal Region and Ductus Deferens of the Turkey (Meleagris gallopavo). J. Anat., 122: 241-252
20. Hodges RD (1974): The Histology of The Fowl. London: Academic Pres. p.: 300-326
21. Janssen SJ, Bunick D, Finnigan-Bunick C, Chen YC, Hess R, Bahr JM (1998): Morphology and Functional of Rooster Efferent Ductule Epithelial Cells in Culture. Tissue Cell., 30: 554-561
22. Karnovsky MJ (1965): Formaldehyde-glutaraldehyde Fixative of High Osmolalitiy for Use in Electron Microscopy. J. Cell Biol., 27: 137A-138A
23. Kiernan JA (1999): Histological & Histochemical Methods. Theory & Practice. Oxford Universty Pres. New York. 3nd Ed.
24. Nickel R, Schummer A, Seiferle E (1977): Anatomy of The Domestıc Birds. Berlin-Hamburg. Verlag Paul Parey. p: 72-75
25. Pelletier MR (1990): A Novel Perspective: The Occluding Zonule Encircles the Apex of the Sertoli Cell as Observed in Bird. Am. J. Anat., 188: 87-108
26. Rikihisa Y, Lin YC (1987): Ultrastructure of the Testis and Epididymis of Japanese Quail (Coturnix coturnix japonica) Administered Gossypol. Poultry Sci., 67: 961- 972
27. Rothwell B, Tingari MD (1973): The Ultrastructure of the Boundary of the Seminiferous Tubule in the Testis of the Domestic Fowl (Gallus domesticus). J. Anat., 114: 321- 328
28. Stefanini MA, Orsi AM, Gregorio EL, Viotto MJS, Baraldi-Artoni SM (1999): Morphologic Study of the Efferent Ductules of the Pigeon (Columba livia). J. Morphol., 242: 247-255
29. Tingari MD (1971): On the Structure of the Epididymal Region and Ductus Deferens of the Domestic Fowl (Gallus Domesticus). J. Anat., 109: 423-435
30. Tingari MD (1972): The Fine Structure of the Epithelial Lining of the Excurrent Duct System of the Testis of the Domestic Fowl (Gallus domesticus). Q. J. Exp. Psychol., 57: 271-295
31. Thurston RJ, Korn N (2000): Symposium: Regulation of Testes Function. Spermiogenesis in Commercial Poultry Species: Anatomy and Control. Poultry Sci. 79: 1650-1668
32. Veneable J, Coggeshall R (1965): A Simplified Lead Citrate Stain for Use in Electron Microscopy. J. Cell Biol., 45: 407-408 Geliş tarihi: 28.12.2009 / Kabul tarihi: 02.09.2010