Porcine circovirus type 2 infection in Turkey

Abstract

Porcine circovirus is the smallest known DNA virus and is identified and characterized by two types: PCV type 1 (PCV 1) and PCV type 2 (PCV 2). Porcine circovirus type 1 (PCV 1) was first identified in 1974 and was recognized as a nondisease-causing agent that frequently occured in laboratory tissue cultures. As for PCV 2, it is defined as an antigenically and genomically different PCV which was commonly seen in swine populations and led to clinic disorders at the end of the 1990s. PCV2 infection in pigs can cause a wide variety of clinical signs and syndromes. This study aims to analyze the presence of PCV 2 related to different clinic cases and syndromes found on some pig farms. To this end, 86 nasal swab samples from two different pig farms and 12 lung tissue pieces from wild boars were collected to detect whether PCV 2 was present. In the study, 98 samples were used in total. The one step PCR technique was applied to the samples obtained. The samples were analyzed in terms of PCV presence; 38 of the samples were identified as PCV positive. Thirty-one of the 38 samples which were identified as PCV positive were identified as PCV 2 in the discriminant diagnosis, while 7 were evaluated as PCV 1

Keywords

• Circovirus
• PMWS
• PCR
• porcine
• Turkey

Türkiye’de domuzlarda circovirus 2 enfeksiyonu

Abstract

Porcine circovirus bilinen en küçük DNA virusu olup PCV tip 1 (PCV 1) ve PCV tip 2 (PCV 2) olmak üzere 2 tipi identifiye ve karakterize edilmiştir. Porcine circovirus tip 1 (PCV 1) ilk defa 1974 yılında identifiye edilmiştir ve laboratuvarda doku kültürlerinde sıklıkla karşılaşılan ve patojen olmayan bir ajan olarak tanımlanmıştır. PCV tip 2 ise 1990’lı yılların sonlarında domuz populasyonlarında yaygın olarak görülen ve klinik hastalık tablosuna neden olan, antijenik ve genomik olarak farklı bir PCV olarak tanımlanmıştır. PCV tip 2 enfeksiyonu domuzlarda çeşitli klinik belirtilere ve sendromlara sebep olabilmektedir. Bu çalışmada bazı domuz yetiştiriciliklerinde farklı klinik hastalık tabloları ve sendromlarla ilişkili bulunan PCV tip 2’nin varlığının araştırılması amaçlanmıştır. Bu amaçla 2 farklı domuz yetiştiriciliğinden toplam 86 adet nasal swap ve 12 adet yaban domuzuna ait akciğer doku parçaları örneklenmiştir. Araştırmada toplam 98 adet örnek kullanılmıştır. Elde edilen örneklere tek basamak PCR tekniği uygulanmıştır. Örnekler öncelikle PCV varlığı yönünden araştırılmış, örneklerden 38 adedi PCV yönünden pozitif olarak tespit edilmiştir. PCV pozitif olarak tespit edilen 38 örneğin 31 adedi ayırıcı tanıda PCV 2 olarak tespit edilmiş, 7 örnek ise PCV 1 olarak değerlendirilmiştir

Keywords

• Circovirus
• domuz
• PMWS
• PZR
• Türkiye

References

1. Allan GM, and Ellis JA (2000): Porcine circoviruses: a review. J Vet Diagn Invest, 12, 3-14.
2. Allan GM, Kennedy S, McNeilly F, Foster JC, Ellis JA, Krakowka SJ, Meehan BM, Adair BM (1999) Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J Comp Pathol, 121, 1-11.
3. Allan GM, McNeilly F, Kennedy S, Daft B, Clarke EG, Ellis JA, Haines DM, Meehan BM, Adair BM (1998): Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the United states of America and Europe. J Vet Diagn Invest, 10, 3-10.
4. Calsamiglia M, Segales J, Quintana J, Rosell C, and Domingo M (2002): Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome. J Clin Microbiol, 1848-1850.
5. Cao S, Chen H, Zhao J, Lü J, iao S, Jin M, Guo A, Wu B, and He Q (2005): Detection of porcine circovirus type- 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting sydrome by multiple PCR. Vet Res Comm, 29, 263-269.
6. Clark EG (1996): Pathology of the post-weaning multisystemic wasting syndrome of pigs. Proc West Can Assoc Swine Pract, 22-25.
7. Clark EG (1997): Postweaning multisystemic wasting sydrome. Proc Annu Meet Am Assoc Swine Pract, 499- 501.
8. Drolet R, Thibault S, D’Allaire S, Thomson JR, Done SH (1999): Porcine dermatitis and nephropathy syndrome (PDNS): An overview of the disease. J Swine Health Prod, 7, 283-285.

9. Ellis J, Hassard L, Clark E, Harding J, Allan G, Wilson P, Strokappe J, martin K, McNeilly F, Meehan B, Todd D, Haines D (1998): Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can Vet J, 39, 44-51.
10. Fenaux M, Halbur, PG, Gill M, Toth TE, Meng XJ (2000): Genetic characterization of type 2 porcine circovirus (PCV 2) from pigs with postweaning multisystemic wasting sydrome in different geographic regions of North america and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV 1 and PCV 2. J Clin Microbiol, 38, 2494-2503.
11. Harding JC (1996): Post-weaning multisystemic wasting syndrome (PMWS):Preliminary epidemiology and clinical presentation. Proc West Can Assoc Swine Pract, 21.
12. Harding J (1997): Post-weaning multisystemic wasting syndrome (PMWS): Preliminary epidemiology and clinical presentation. Proc West Can Assoc Swine Pract, 28, 503.
13. Harding JCS, Clark EG (1997): Recognizing and diagnosing post-weaning multisystemic wasting sydrome (PMWS). Swine Health Prod, 5, 201-203.
14. Huang C, Hung JJ, Wu CY, Chien MS (2004): Multiplex PCR for rapid detection of pseudorabies virus, porcine parvovirus and porcine circoviruses. Vet Microbiol, 101, 209-214.
15. Kennedy S, Moffett D, McNeilly E, Meehan, Ellis J, Krakowka S, Allan GM (2000): Reproduction of lesions of postweaning multisystemic wasting syndrome by experimental infection of conventional pigs with porcine circovirus type 2 alone or in combination with porcine parvovirus. J Comp Pathol, 122, 9-24.
16. Kim J, Chung H.-K, Jung T, Cho W.-S, Choi C, Chae C (2002): Postweaning multisystemic wasting syndrome of pigs in Korea: prevalance, microscopic lesions and coexisting microorganisms. J Vet Med Sci, 64, 57-62.
17. Larochelle R, Antaya M, Morin M, Magar R (1999): Typing of porcine circovirus in clinical specimens by multiplex PCR. J Virol Methods, 80, 69-75.
18. Madec F, Eveno E, Morvan P, Hamon L, Blanchard P, Cariolet R, Amenna N, Morvan H, Truong C, Mahe D, Albina E, Jestin A (2000): Post-weaning multisystemic wasting syndrome (PMWS) in pigs in France: clinical observations from follow-up studies on affected farms. Livestock Prod Sci, 63, 223-233.
19. Mc Neilly F, Kennedy S, Moffet D, Meehan BM, Foster JC, Clarke EG, Ellis JA, Haines DM, Adair BM, Allan GM (1999): A Comparison of in situ hybridization and immunohistochemistry for the detection of a new porcine circovirus in formalin-fixed tissues from pigs with post- weaning multisystemic wasting syndrome (PMWS). J Virol Methods 80, 123-128.
20. Mc Nulty M, Dale J, Lukert P, Mankertz A, Randles J, Todd D (2000) Circoviridae. In Virus Taxonomy: Classification and nomenclature of viruses the seventh report of the International committee on taxonomy of viruses. MHV van Regenmortel, CM Fauquet, DHL Bishop, CH Calisher, EB Carstens, MH Estes, SM Lemon, J Maniloff, MA Mayo, DJ McGeoch, CR Pringle, RB Wickner, eds. San Diego: Academic Press, pp. 299-303.
21. Meehan BM, McNeilly F, Todd D, Kennedy S, Jewhurst VA, Ellis JA, Hassard LE, Clark EG, Hanes DM, and Allan GM (1998): Characterization of novel circovirus DNAs associated with wasting sydromes in pigs. J Gen Virol, 79, 2171-2179.
22. Morozov I, Sirinarumiter T, Sorden SD, Halbur PG, Morgan MK, Yoon KJ, Paul PS (1998): Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J Clin Microbiol, 36, 2535-2541.
23. Onuki A, Abe K, Togashi K, Kawashima K, Tanehi A, Tsunemitsu H (1999): Detection of porcine circovirus from lesions of a pig with wasting disease in Japan. J Vet Med Sci, 61, 1119-1123.
24. Ouardani M, Wilson L, Jette R, Montpetit C, Dea S (1999): Multiplex PCR for detection and typing of porcine circoviruses. J Clin Microbiol, 37, 3917-3924.
25. Pallares FJ, Halbur PG, Oprıessnıg T, Sorden SD, Villar D, Janke BH, Yaeger MJ, Larson DJ, Schwartz KJ, Yoon KJ, Hoffman LJ (2002): Porcine circovirus type 2 (PCV 2) coinfections in US field cases of postweaning multisystemic wasting syndrome (PMWS). J Vet Diagn Invest, 14, 515-519.
26. Rosell C, Segales J, Plana-Duran J, Balasch M, Rodriguez-Arrioja GM, Kennedy S, Allan GM, MNeilly F, Latimer KS, Domingo M (1999): Pathological, immunohistochemical, and in-situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome (PMWS) in pigs. J Comp Pathol, 120, 59-78.
27. Rosell C, Segales J, Ramos-Vara JA, Folch JM, Rodriguez-Arrioja GM, Duran CO, Balasch M, Plana- Duran j, Domingo M (2000): Identification of porcine circovirus in tissue of pigs with porcine dermatitis and nephropathy syndrome. Vet Rec, 146, 40-43.
28. Smith WJ, Thomson JR, Done S (1993): Dermatitis/ nephropathy syndrome of pigs. Vet Rec, 132, 47.
29. Thibault S, Drolet R, Germain MC, D’Allaire S, Larochelle R, Magar R (1998): Cutaneous and systemic necrotizing vasculitis is swine. Vet Pathol, 35, 108-116.
30. Thomson JR, Lainson FA, Thomson N, Donachie W (1998): A study of Pasteurella multocida as a possible aetiological agent in porcine immune complex glomerulonephritis and dermatitis syndrome. In: Dore S, Thomson J, and Varley M (eds), Proceedings of the 15th IPVS Congress, vol. 3, p.396. Nottingham University press, Nottingham.
31. Tischer I, Bode l, Peters D, Pociuli S, Germann B (1995): Distribution of antibodies to porcine circovirus in swine populations of different breeding farms. Arch Virol, 140, 737-743.
32. Tischer I, Gelderblom H, Vettermann W, and Koch MA (1982): A very small porcine circovirus with circular single-stranded DNA. Nature, 295, 64-66.
33. Tischer I, Rasch R, Tochtermann G (1974): Characterization of papovavirus- and picornavirus-like particles in permanent pig kidney cell lines. Zentralbe Bakteriol Org A, 226, 153-167.
34. West KH, bystrom JM, Wojnarowicz C, Shantz N, Jacobson M, Allan GM, Haines DM, Clark EG, Krakowka S, McNeilly F, Konoby C, Martin K, Ellis JA (1999): Myocarditis and abortion associated with intrauterine infection of sows with porcine circovirus 2. J Vet Diagn Invest, 11, 530-532.