Effects of oral zinc sulfate applications at different pH (ascorbic acid, vinegar of grapes and distillated water) on serum zinc levels in rabbits

Abstract

The oral zinc (Zn) applications may be required in the case of Zn losses in the body. Lots of Zn forms and compounds are used for this aim. Sulfate form of Zn is used in a widespread manner with in distillated water or other diluent solutions. The purpose of this study is to reveal the effect of some acidifier agents as diluent solutions (therapeutic dose of ascorbic acid in distillated water and pure vinegar of grapes) versus distillated water, well known and commonly used. Thirty New Zealand rabbits in three equal groups were used in this study. Zinc sulfate solutions with different diluents as above were given to groups orally. Before and after (2,5 hours later) oral applications, blood samples were collected via cardiac puncture under general diethyl ether anesthesia. Atomic absorption spectrometer equipped with flame system was employed. Significant differences (p≤0.001) were found between first and last serum Zn levels in all groups. There were also significant differences among last samples of groups (minimum p<0.05). Throughout the experiment time significant differences were found between control group and acidifier groups (ascorbic acid and vinegar of grapes) (p<0.05), but not between two acidifier groups (p>0.05). Significant correlation was detected between pH and serum Zn levels of oral solutions (r = -0.838, p<0.001). It was also observed that the oral solutions pH of ZnSO4influenced the serum Zn level significantly (r2: 70.1%, p<0.001). Consequently, the acidity of oral ZnSO4 fortification solution affected the serum Zn level. While the pH level of oral ZnSO4solutions was decreasing, serum Zn level increased. The therapeutic dose of ascorbic acid in distillated water was more effective than pure vinegar of grapes

Keywords

• Ascorbic Acid
• serum Zn level
• rabbit
• vinegar of grapes
• Zn fortification

Tavşanlarda farklı pH’da (askorbik asit, üzüm sirkesi ve saf suya) oral çinko sülfat uygulamalarınınserum çinko düzeyleri üzerine etkileri

Abstract

Vücutta çinko (Zn) kaybının olması durumunda oral Zn uygulamalarına gerek duyulabilir. Bu amaçla çok sayıda Zn formu veya bileşiği kullanılmaktadır. Zn’nin sülfat formu geniş çapta safsu veya diğer sulandırma solüsyonları ile birlikte yaygın bir şekilde kullanılmaktadır. Bu çalışmanın amacı, sulandırma solüsyonları halinde bazı asitleştirici ajanların (saf su içinde tedavi dozunda askorbik asit ve saf üzüm sirkesi) iyi bilinen ve yaygın bir şekilde kullanılan saf suya karşı etkisinin ortaya konmasıdır. Bu çalışmada üç eşit grup halinde 30 Yeni Zelanda ırkı tavşan kullanıldı. Yukarıda bahsedildiği gibi farklı sulandırmalar ile ZnSO4gruplara oral yolla verildi. Oral uygulama öncesi ve 2,5 saat sonrası dietil eter genel anestezisi altında kalpten kan örnekleri alındı. Alev sistemli atomik absorbsiyon spektrofotometre kullanıldı. Tüm gruplarda ilk ve son serum Zn düzeyleri arasındaki fark önemli bulundu (p≤0.001). Grupların son örneklerinin arasında da önemli farklılıklar vardı (en az p<0.05). Deney süresi boyunca kontrol grubu ve asit grupları (askorbik asit ve üzüm sirkesi) arasında önemli farklılık bulundu (p<0.05), fakat iki asit grubu arasında fark gözlenmedi (p>0.05). Oral solüsyon pH’ı ve serum Zn düzeyi arasında önemli korelasyon tespit edildi (r = -0.838, p<0.001). Aynı zamanda ZnSO4 oral solüsyon pH’ının serum Zn düzeyini önemli ölçüde etkilediği gözlendi (r2: 70.1%, p<0.001). Sonuç olarak, oral ZnSO4 takviyesinin asiditesi serum Zn düzeyini etkiledi. Oral ZnSO4 solüsyonunun pH’ı azalırken, serum Zn düzeyi arttı. Distile su içinde askorbik asitin terapotik dozu saf üzüm sirkesinden daha etkili bulundu

Keywords

• Askorbik asit
• serum Zn düzeyi
• tavşan
• üzüm sirkesi
• Zn takviyesi

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