Tavşanlarda deneysel pastörelloziste patolojik bulgular

Abstract

Bu çalışmada, Yeni Zelanda Beyazı 6-8 haftalık tavşanlara intranazal, intratraheal ve intravenöz olarak tavşan orijinli Pasteurella multocida 3:A serotipi inoküle edildi. Hayvanlar inokülasyon sonrası 4-6, 8-10 ve 14. günlerde öldü ya da ötanazi uygulandı. Tüm dokular makroskobik ve mikroskobik olarak muayene edildi. Bunlara ilaveten, akciğer lezyonları elektron mikroskobik olarak incelendi. Akciğer lezyonları en fazla intratraheal inokülasyon grubunda, daha az olarak intranazal inokülasyon grubunda ve en az olarak da intravenöz olarak inoküle edilen tavşanlarda görüldü. Pasteurella multocida 3:A serotipi inoküle edilmiş tüm gruplarda özellikle aşağı solunum sistemi lezyonları şekillendi. Akciğer ve plöra lezyonları intranazal olarak inoküle edilmiş hayvanların %50’sinde, intratraheal olarak inoküle edilmiş hayvanların %75’inde ve intravenöz olarak inoküle edilmiş hayvanların ise %17’sinde görüldü. Tavşanlarda histolojik olarak şiddetli fibrinopurulent ve nekrotik pnömoni, fibrinopurulent plöritis ve fibrinopurulent perikarditise rastlandı. Pasteurella multocida’lara elektron mikroskobik olarak serbest halde alveol boşluklarında ve fagosite edilmiş halde kapillar lumenlerindeki ve alveol boşluklarındaki nötrofil granülositlerde rastlandı. Sonuç olarak, Pasteurella multocida 3:A serotipi tavşanlara hangi yolla inoküle edilirse edilsin pnömoni oluşturulabildiği fakat en etkili yolun intratraheal yol olduğu, bunu sırası ile intranazal ve intravenöz yolun izlediği belirlendi

Keywords

• Elektron mikroskopi
• Pasteurella multocida
• pastörellozis
• tavşan

Pathological findings of experimental pasteurellosis in rabbits

Abstract

In this study, three groups of New Zealand White rabbits, 6-8-week-old, were inoculated with Pasteurella multocida serotype 3:A strain of rabbit origin by routes of intranasal, intratracheal and intravenous inoculations, respectively. The animals died or killed after the 4-6, 8-10 and 14 days of observation periods were necropsied. All of tissues were examined macroscopically and microscopically. In addition, pulmonary lesions were examined with electron microscopically. Pneumonic lesions were detected most consistently in rabbits inoculated intratracheally. Intranasal inoculation was less effective than intratracheal inoculation. The lesions were minimal in intravenous dosed group. All of the routes of Pasteurella multocida serotype 3:A inoculations produced especially the lower respiratory tract lesions. Pulmonary lesions were observed in %50 of animal inoculated intranasally, %75 of animals inoculated intratracheally and %17 of animals inoculated intravenously. The histological examinations revealed severe fibrinopurulent and necrotic pneumoni, fibrinopurulent pleuritis and fibrinopurulent pericarditis. Pasteurella multocida were shown electron microscopically in free manner in alveolar spaces, in phagocytic vacuoles of cytoplasm of neutrophilic granulocytes in alveolar spaces and in alveolar capillary lumens. As a result, Pasteurella multocida 3:A serotype can induce pneumonia in rabbits through different routes of inoculation: intratracheal, intranasal, and intravenous. However, intratracheal inoculation is the most effective method to induce pneumonia while intranasal inoculation is the second most and intravenous inoculation is the least effective

Keywords

• Electron microscopy
• Pasteurella multocida
• pasteurellosis
• rabbit

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