Effect of recombinant follicle stimulating hormone (rFSH) on some fertility parameters in Awassi ewes synchronized with PGF2α in the breeding season

Abstract

The present study was aimed at the investigation of the effects of Recombinant Follicle Stimulating Hormone (rFSH) on fertility in Awassi ewes synchronized with PGF2α in the breeding season. The fifty thoroughbred Awassi ewes used in the study were injected twice with 0.150 mg of Tiaprost (PGF2α, Iliren®) at a 10-day interval. Following the second PGF2α injection, the ewes were randomly distributed into two equal groups. The ewes included in the first group were not subjected to any additional injection and were referred to as the PGF2α group (Group 1, n=25). The animals included in the second group (Group 2, n=25) were administered 10 IU Follitropin (rFSH, Puregon®), diluted 1:10 with physiological saline, 24 hours before the second PGF2α injection. Ewes were tested for estrus by a fertile teasing ram three times a day (06.00, 14.00 and 20.00 h), for one hour each time. Ewes exhibiting estrus were mated naturally. Pregnancy was determined by transrectal ultrasonography on 40. d after mating. Estrus was observed at a rate of 88.0 % in Group 1 and 92.0 % in Group 2. The rate of pregnancy was 100 % in the first group and 95.6% in the second group. The rate of lambing was 100 % and 95.5 % in Groups 1 and 2, respectively. Furthermore, twin pregnancy rates were 4.5 % in the first group and 57.1% in the second group. While differences between the groups were determined to be significant (p<0.001), no significant difference was detected for pregnancy, estrus or lambing rates. In conclusion, it was determined that the administration of rFSH to Awassi ewes in the breeding season, 24 hours before the second PGF2α injection, led to an increase in twin pregnancy rates. Thereby, this protocol can be used as an alternative synchronization method to increase fertility. It has been concluded that further studies are needed to determine the effect of different doses of rFSH on fertility in ewes

Keywords

• Awassi ewes
• fertility
• PGF2α
• rFSH

Aşım sezonunda PGF2α ile senkronize edilen İvesi koyunlarında bazı fertilite parametreleri üzerinerekombinant follikül stimüle edici hormonun (rFSH) etkisi

Abstract

Sunulan çalışmanın amacı, aşım sezonunda PGF2α ile senkronize edilmiş İvesi Koyunlarında Recombinant Follikül Stimüle Hormonun (rFSH) fertilite üzerine etkisi araştırmaktır. Çalışmada kullanılan toplam 50 baş safkan İvesi koyuna 10’ar gün ara ile iki defa 0.150 mg dozunda Tiaprost (PGF2α, Iliren®) enjeksiyonları yapıldı. İkinci PGF2α enjeksiyonları yapıldıktan sonra koyunlar rastgele iki eşit gruba ayrıldı. Birinci gruptaki koyunlara ilave bir enjeksiyon yapılmadan (Grup 1, n=25) PGF2α grubu, ikinci gruptaki (Grup 2, n=25) koyunlara ise ikinci PGF2α enjeksiyondan 24 saat önce 1/10 oranında serum fizyolojik ile sulandırılıp 10 IU Follitropin (rFSH, Puregon®) enjeksiyonu yapıldı. Östruslar (Saat 06.00, 14.00 ve 20.00) günde üç kez 1’er saat süreyle fertil bir arama koçu tarafından test edildi. Östrus gösteren koyunlara doğal aşım yaptırıldı. Gebelikler doğal aşımı takip eden 40. günde transrektal ultrasonografi ile tespit edildi. Östruslar Grup 1’de % 88.0, Grup 2’de % 92.0 oranında gözlendi. Gebelik oranı ise birinci grupta % 100 elde edilirken bu oran ikinci grupta % 95.6 olarak elde edildi. Kuzulama oranı Grup 1’de % 100 Grup 2’de ise % 95.5 olarak gerçekleşti. İkizlik oranları birinci grupta % 4.5, ikinci grupta % 57.1 olarak gerçekleşti. Gruplar arasında farkın önemli olduğu saptanırken (p<0.001), gebelik, östrus ve kuzulama oranları açıdan önemli bir fark saptanmadı. Sonuç olarak, aşım sezonundaki İvesi Koyunlarında ikinci PGF2α enjeksiyondan 24 saat önce rFSH uygulamalarının ikizlik oranını arttırdığı görülmüştür. Böylece bu protokol, fertiliteyi arttırmak amacıyla alternatif bir senkronizasyon metodu olarak kullanılabilir. Koyunlarda rFSH nın farklı doz uygulamalarının fertilite üzerine etkisini belirlemek için daha fazla çalışmaların yapılma kanaatine varılmıştır

Keywords

• İvesi koyun
• fertilite
• PGF2α
• rFSH

References

1. Ahmad N, Lewis PE, Walker CZ, Alt FL, Isnkeep EK (1996): FSH in propylene glycol on ovulation rate and prolificacy in anestrous ewes. J Anim Sci, 74, 212 (Abstract).
2. Ahmed MMM, Makawi SE, Jubara AS (1998): Synchronization of oestrus in Nubian goats. Small Rumin Res, 30, 113–120.
3. Ali A, Sirard MA (2002): Effect of the absence or presence of various protein supplements on further development of bovine oocytes during in vitro maturation. Biol Reprod, 66, 901-905.
4. Anderiesz C, ferraretti AP, Magli C, Fiorentino A, Fortini D, Gianaroli L, Jones GM, Trounson AO (2000): Effect of recombinant human gonadotropins on human, bovine and murine oocyte meiosis, fertilization and embryonic development in vitro. Hum Reprod, 15, 1140- 1148.
5. Ataman MB, Aköz M (2006): GnRH-PGF2α and PGF2α- PGF2α synchronization in Akkaraman cross-bred sheep in the breeding season. Bull Vet Inst Pulawy, 50, 101-104.
6. Bodin L, Drion PV, Remy B, Brice G, Cognie Y, Beckers JF (1997): Anti-PMSG antibody levels in sheep subjected annually to estrus synchronization. Reprod Nutr Dev, 37, 651-660.
7. Boscos C, Samartzi FC, Dellis S, Rogge A, Stefanakis A, Krambovitis E (2002): Use of progestagen- gonadotrophin treatments in estrus synchronization of sheep. Theriogenology, 58, 1261-72.
8. Boscos C, Vainas E, Kouskoura TH, Samartzi F, Vafiadis D, Cellis S (1997): Superovulatory response of Chios and Friesian ewes to two FSH dose levels, Reprod Dom Anim, 32, 195-198.

9. Campbell BK, Dobson H, Scaramuzzi RJ (1998): Ovarian function in ewes made hypogonadal with GnRH antagonist and stimulated with FSH in the presence or absence of low amplitude LH pulses. J Endoc, 156, 213– 222.
10. Cardwell BE, Fitck GQ, Geisert RD (1998): Ultrasonic evaluation for the time of ovulation in ewes treated with norgestomet and norgestomet followed by pregnant mare’s serum gonadotropin. J Anim Sci, 76, 2235-2238.
11. Choi YH, Carnevale EM, Steidel GE, Squires J, Squires EL (2001): Effects of gonadotrophins on bovine oocytes matured in TCM-199. Theriogenology, 56, 661- 670
12. Cline AM, Ralston NJ, Seals CR, Lewis SG (2001): Intervals from norgestomet withdrawal and injection of equine chorionic gonadotropin or P.G. 600 to estrus and ovulation in ewes. J Anim Sci, 79, 589–594.
13. D’Alessandro AG, Martemucci G, Colonna MA, Borghese A, Terzano MG, Bellitti A (2001): Superovulation in ewes by a single injection of pFSH dissolved in polyvinylpyrrolidone (PVP): Effects of PVP molecular weight, concentration and schedule of treatment. Anim Reprod Sci, 65, 255–264.
14. Dattena M, Vespignani S, Branca A, Gallu, M, Ledda S, Naitana S, Cappai P (1994): Superovulatory response and quality of embryos recovered from anestrus ewes after a single injection of porcine FSH dissolved in polyvinylpyrrolidone. Theriogenology, 42, 235–239.
15. Driancourt MA (2001): Regulation of ovarian follicular dynamics in farm animals. Implications for manipulation of reproduction. Theriogenology, 55, 1211–1239.
16. Duggavathi R, Bartlewski PM, Agg E, Flint S, Barrett DMW, Rawlings NC (2005): The effect of the manipulation of follicle-stimulating hormone (FSH)-peak characteristics on follicular wave dynamics in sheep: Does an ovarian-ındependent endogenous rhythm in FSH secretion exist? Biol Reprod, 72, 1466–1474
17. Fonseca JF, Bruschi JH, Zambrini FN, Demczuk E, Viana JHM, Palhão MP (2005): Induction of synchronized estrus in dairy goats with different gonadotropins. Anim Reprod, 2, 50-53.
18. Freitas VJF, Baril G, Saumande J (1997): Estrus synchronization in dairy goats: use of fluorogestone acetate vaginal sponges or norgestomet ear implants. Anim Reprod Sci, 46, 237–244.
19. Fukui Y, Ishikawa D, Ishida N, Okada M, Ryoko Itagaki R, Ogiso T (1999): Comparison of fertility of estrous synchronized ewes with four different intravaginal devices during the breeding season. J Reprod Dev, 45, 337–343.
20. Garcia CV, Boime I (2001): Novel recombinant gonadotropins. Trends Endocrin Met, 12, 72–77.
21. Godfrey WR, Gray ML, Collins RJ (1997): A comparison of two methods of estrus synchronization of hair sheep in the tropics. Anim Reprod Sci, 47, 99-106.
22. Hackett AJ, Langford GA, Robertson HA (1981): Fertility of ewes after syncronization of estrus with prostaglandin F2α and artificial insemination. Theriogenology, 15, 599-603.
23. Hashemi M, Safdarian M, Kafi M (2006): Estrous response to synchronization of estrus using different progesterone treatments outside the natural breeding season in ewes. Small Rum Res, 65, 279–283.
24. Howles CM (1996): Genetic engineering of human FSH (Gonal-F). Hum Reprod, 2,172-191.
25. Khan HT, Hastie MP, Beck GFN, and Khalid M (2003): hCG treatment on day of mating improves embryo viability and fertility in ewe lambs. Anim Reprod Sci, 76, 81-89.
26. Knights M, Baptiste QS, Dixon AB, Pate JL, Marsh DJ, Inskeep EK and Lewis PE (2003): Effects of dosage of FSH, vehicle and time of treatment on ovulation rate and prolificacy in ewes during the anestrous season. Small Rum Res, 50, 1-9.
27. Lemke EP, Adams BM, -Shariff1 AJ, Boime1 I, Adams TE (2008): Single-chain human gonadotropin analogs induce follicle development in sheep. J Endoc, 196, 593– 600
28. Lindsell CE, Rajkumar K, Manning AW, Emery SK, Mapletof RJ, Murphy BD (1986): Variability in FSH:LH rations among batches of commercially avaliable gonadotrophins. Theriogenelogy, 25, 167.
29. Lopez-Sebastion A, Gomez-Brunet A, Lishman AW, Johson SK, Inskeep EK (1993): Modification by propylene glycol of ovulation rate in ewes in response to a single injection of FSH. J Reprod Fertil, 99, 437-442.
30. Loubser PG, Van Niekerk CH (1981): Oestrus synchronization in sheep with progesterone-impregnated (MAP) intravaginal sponges and a prostaglandin analogue. Theriogenology, 15, 547-552.
31. Lunenfeld B (2004): Historical perspectives in gonadotrophin therapy. Hum Reprod, 10, 453–467
32. McNatty, KP Hudson N, Gibb M, Ball K, Henderson KM, Heath DA, Lun S Kieboom LE (1985): FSH influences follicle viability, estradiol biosynthesis and ovulation rate in Romney ewes. J Reprod Fertil, 75, 121– 131
33. Menchaca A, Miller V, Gil J, Pinczak A, Laca M, and Rubianes E (2004): Prostaglandin F2a treatment associated with timed artificial ınsemination in ewes. Reprod Dom Anim, 39, 352-355.
34. Moor RM, Kruip Tham, Gren D (1984): Intraovarian control of folliculogenesis. Limits to superovulation?. Theriogenology, 21, 103-16
35. Moor RM, Trouson AO (1997): Hormonal and follicular factors affecting maturation of sheep oocytes in vitro and their subsequent developmental capacity. J Reprod Fertil, 49, 101-109.
36. Picton HM, Tsonis CG, McNeilly AS (1990): FSH causes a time-dependent stimulation of preovulatory follicle growth in the absence of pulsative LH secretion in ewe chronically treated with gonadotrophin-releasing hormone agonist. J Endoc, 126, 297–307.
37. Pierce JG, Parsons TF (1981): Glycoprotein hormones: structure and function. Annu Rev Bıochem, 50, 465–495.
38. Romano JE (1998): Effect of two doses of cloprostenol in two schemes for estrus synchronization in Nubian goats. Small Rumin Res, 28,171–176.
39. Russel AJF, Doney JM and Gunn RG (1969): Subjective assessment of body fat in live sheep. J Agric Sci. Camb, 72, 451-454.
40. Simonetti L, Forcada F, Rivera OE, Carou N, Alberio RH, Abecia JA, Palacin I (2008): Simplified superovulatory treatments in Corriedale ewes. Anim Reprod Sci, 104, 227–237.
41. SPSS, 13.0 for window; Inc., Chicago.
42. Tsakalof P, Vlachos N, Latoutsakis D (1997): Observation on the reproductive performans of ewe lambs synchronized for estrus. Vet Rec, 100, 1205-1211.