Detection of exfoliative toxin in Staphylococcus spp. isolated from skins of healthy dogs and dogs with skin lesions

Abstract

The aim of this study was to determine the presence of exfoliative toxin in Staphylococcus species isolated from nose and skins of 50 healthy dogs and skin lesions of 50 dogs at different race, age and gender; and to investigate the nasal carriage of Staphylococcus species in dogs. A total of 135 Staphylococcus intermedius (67.5 %), 12 S. aureus (6 %), 2 S. chromogenes (1 %) and 1 S. capitis (0.5 %) were isolated from 200 skin and nose samples. No bacterial growth was observed in 50 (25 %) samples. Among the isolated staphylococci, exfoliative toxin was not determined in 2 S. chromogenes and 1 S. capitis isolates but it was determined phenotypically in 54 S. intermedius isolates and both phenotypically and genotypically in 12 S. aureus isolates. As a result; it was determined that S. intermedius and S. aureus strains isolated in this study produced exfoliative toxin and because of their high isolation rates the opinion of their role in the etiology of canine pyoderma and importance of nasal carriage was supported. In this study the Staphylococcus spp. isolated from both skin and nasal mucosa samples of the same dog were compared for the first time and also it was determined that S. aureus strains isolated from dog skin produced exfoliative toxin for the first time

Keywords

• Dog
• exfoliative toxin
• isolation
• pyoderma
• Staphylococcus spp

Sağlıklı ve deri lezyonlu köpeklerin derilerinden izole edilen stafilokok türlerinde eksfoliatif toksinvarlığının belirlenmesi

Abstract

Bu çalışmada farklı ırk, yaş ve cinsiyetteki 50 sağlıklı ve 50 deri lezyonu bulunan köpeğin burun ve derilerinden izole edilen stafilokok türlerinde eksfoliatif toksin varlığının belirlenmesi, izole edilen stafilokok türlerinin köpeklerdeki burun boşluğunda etken taşıyıcılığının araştırılması amaçlandı. Toplanan 200 deri ve burun örneğinden 135’inde S. intermedius (% 67.5), 12’sinde S. aureus (% 6), 2’sinde S. chromogenes (% 1) ve 1’inde S. capitis (% 0.5) saptanırken 50 (% 25) örnekte üreme olmadığı görüldü. İzole edilen stafilokoklardan, 2 S. chromogenes ve 1 S. capitis izolatında eksfoliatif toksin varlığı belirlenemedi ancak 54 S. intermedius izolatının fenotipik olarak, 12 S. aureus izolatının ise hem fenotipik hem de genotipik olarak eksfoliatif toksin ürettiği saptandı. Sonuç olarak, bu çalışmada izole edilen S. intermedius ve S. aureus’ların eksfoliatif toksin sentezleyebildikleri gösterilmiş olup yüksek izolasyon oranlarından dolayı kanin piyodermaların etiyolojisinde rol aldıkları ve burun boşluğunda etken taşıyıcılığının önemli olduğu görüşü desteklendi. Bu çalışmada ilk defa, aynı köpeğin hem derisinden hem de burun mukozasından örnek alınarak üreyen stafilokok türleri karşılaştırılmış ve yine ilk defa bu çalışmada, köpek derisinden izole edilen S. aureus suşlarının eksfoliatif toksin sentezlediği belirlenmiştir

Keywords

• Eksfoliatif toksin
• izolasyon
• köpek
• piyoderma
• Staphylococcus spp

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