Detection of pathomorphological and immunohistochemical findings of tuberculosis in cattle slaughtered in Ankara and its surroundings

Abstract

Macroscopical and microscopical lesions were examined that 4-6 years old, different sex and breeds, 86 of cattle which detected tuberculosis from all materials were collected from slaughterhouses of Ankara and its surroundings. Generally 78 (91%) were located in the lungs, pleura and regional lymph nodes, 5 (6%) were located in the liver and portal lymph nodes, and 3 (3%) were generalised. Pathomorphologically, 81 (94%) comprised miliar-nodular tubercles, and 5 (6%) were determined to be chronic organ tuberculosis. Ziehl-Neelsen (ZN) stained sections revealed acide fast bacille (AFB) in lung, lymph nodes, liver and trachea (96%). Immunohistochemically, M. bovis antigens were seen in the macrophages, necrotic areas, and the Langhans giant cells (96%) in the same tissues but generally the number and intensity of the positivity were much higher than ZN

Keywords

• Cattle
• immunoperoxidase
• pathology
• tuberculosis

Ankara ve çevresinde bulunan mezbahalarda kesilen sığırlarda saptanan tüberkülozunpatomorfolojik ve immunohistokimyasal bulgularının incelenmesi

Abstract

Bu çalışmada Ankara ve çevre il/ilçe mezbahanelerinde kesilen 4-6 yaşlı, farklı ırk ve cinsiyette 86 tüberkülozlu sığırda saptanan makroskobik ve mikroskobik lezyonlar incelendi. Lezyonların 78’inin (%91) akciğer, plöra ve bölge lenf düğümlerinde, 5’inin (%6) karaciğer ve portal lenf düğümlerinde bulunduğu, 3’ünün (%3) ise generalize olduğu görüldü. Bunlardan, 81’i (%94) milier-nodüler tüberküller ve 5’i (%6) kronik organ tüberkülozu şeklindeydi. Bu hayvanların %96’sında akciğer, bölge lenf düğümleri, karaciğer ve traheadan yapılan Ziehl-Neelsen (ZN) boyamada aside dirençli basiller gözlendi. İmmunohistokimyasal olarak da olguların %96’sında aynı dokularda M. bovis antijenleri makrofajların sitoplazmasında, nekrotik alanlarda serbest halde ve Langhans dev hücrelerinde dikkati çekti. Her iki boyamada aynı oranda etken boyanması saptanmasına rağmen genel olarak immunohistokimyasal boyamalarda etken hem çok daha belirgindi hem de pozitiflik daha yoğundu

Keywords

• İmmunoperoksidaz
• patoloji
• sığır
• tüberküloz

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