Distemper virus-induced apoptotic changes in cerebellum

Abstract

In the study, it was purposed that detection of apoptotic changes with terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick end-labelling (TUNEL) method in the cerebellum of 10 dogs (n:3 females, n:7 males), varied 3 months to 2.5 years-old, different breed (1 Husky, 9 Mongrel) and sex (3 females, 7 males) in distemper disease. Histopathologically, acute (n:4) and chronic (n:6) nonpurulent encephalitis, moderate and/or mild demyelination, and intranuclear inclusion bodies were seen. Immunohistochemically distemper virus antigen was also detected in demyelinated white matter, astrocytes, and inclusion bodies in all dogs by using monoclonal canine distemper virus nucleoprotein sera. Morphologically multiple consendations of nuclear chromatin in astrocytes were found with Cresyl violet stain. TUNEL reaction in markedly astrocytes localized surrounding the demyelination areas, rarely inclusion bodies, gemistocytic astrocytes, Gitter cells in white matter and granular layer of cerebellum was demonstrated. The reactions in chronic inflammatory lesions were also detected in perivascular mononuclear cells

Keywords

• Dog
• distemper virus
• apoptosis
• TUNEL

Distemper virusuna bağlı serebellumda oluşan apoptotik değişiklikler

Abstract

Bu çalışmada, canine distemper virusu belirlenen 3 ay-2.5 yaşlı, değişik ırk (1 Husky, 9 melez) ve cinsiyetteki (3 dişi, 7 erkek) 10 adet köpeğin serebellumunda TUNEL (terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick endlabeling) metodu ile gözlenen apoptotik değişiklikler araştırıldı. Histopatolojik incelemede 4 hayvanda akut, 6 hayvanda da kronik nonpurulent ensefalitise ve demyelinasyona (hafif ve/veya orta şiddette) ilgili bulgular gözlendi. Monoclonal canine distemper virus nucleoprotein serumu kullanılarak yapılan immunohistokimyasal boyamada demyelinize beyaz maddede, astrositlerde ve inkluzyon cisimciklerinde distemper virusu antijeni saptandı. Morfolojik olarak Cresyl violet boyamasında, astrositlerde kromatinin kaba partiküller halinde çekirdek membranına yakın olarak toplandığı görüldü. TUNEL metodu ile yapılan boyamalarda ise akut olgularda daha yoğun olmak üzere özellikle demyelinize alanların etrafında bulunan astrositlerde, daha nadir olarak da inkluzyon cisimciklerinde, gemistositik astrositlerde, Gitter hücrelerinde, granuler tabaka hücrelerinde ve perivasküler mononüklear hücrelerde apoptotik değişiklikler saptandı

Keywords

• Apoptozis
• distemper virus
• köpek
• TUNEL

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