The investigation of the presence of bovine herpesvirus type 4 (BoHV-4) in cows with metritis in a dairy herd

Abstract

To investigate the occurrence of bovine herpesvirus type 4 (BoHV-4) infection in a dairy herd including cows with metritis, various methods were used for the diagnosis BoHV-4 in vaginal discharge samples. Blood serum samples were obtained from 368 cows for serodiagnosis of BoHV-4 infection. Of 368 serum samples, 256 (69.6%) were found positive for BoHV4 specific antibodies. For the virological study, vaginal discharge samples of 55 cows with metritis were used and tested for BoHV-4 using polymerase chain reaction (PCR), virus isolation and immunofluorescence techniques. Twenty-nine percent (16/55) of the vaginal discharge samples obtained from cows with metritis were found positive for BoHV-4 DNA by PCR. In this study, the presence of BoHV-4 was determined in cows with metritis in post-partum period in a dairy herd

Keywords

• Antibody
• bovine herpesvirus type 4
• metritis
• polymerase chain reaction
• sequence analysis

Bir süt sığırcılığı işletmesinde metritisli ineklerde BoHV-4 ün varlığının araştırılması

Abstract

Metritis olgularının gözlendiği bir sütçü sığır sürüsünde, örneklenen vajinal svaplarda farklı tanı yöntemleriyle BoHV-4 infeksiyonunun varlığı araştırıldı. BoHV-4 ün serolojik tanısı amacıyla 169 adedi metritis semptomları gösteren, 199 adedi ise metritisli hayvanlarla birlikte barındırılan sağlıklı görünümlü hayvanlar olmak üzere toplam 368 adet sığırdan kan serum örneği alındı. Virolojik kontrol amacıyla, metritisli 55 adet inekten alınan vajinal svap örnekleri polimeraz zincir reaksiyonu, virus izolasyonu ve immunfloresan tekniği ile BoHV-4 yönünden araştırıldı. 368 serum örneğinin 256 adedi (%69.6) BoHV-4 spesifik antikorları yönünden pozitif bulundu. Seropozitiflik oranları, metritisli ve metritisli hayvanlarla birlikte barındırılan sağlıklı görünümlü hayvanlarda sırasıyla, %69.8 ve %69 olarak tespit edildi. Metritisli hayvanlardan alınan vajinal svap örneklerinin %29 (16/55)'unun BoHV-4 DNA'sı yönünden PCR tekniği ile pozitif olduğu saptandı. Bu çalışmada, bir süt sığırcılığı işletmesinde bulunan doğum sonrası dönemdeki metritis problemli ineklerde BoHV-4 ün varlığı belirlenmiştir

Keywords

• Antikor
• bovine herpesvirus tip 4
• dizin analizi
• metritis
• polimeraz zincir reaksiyonu

References

1. Bartha A, Juhasz M, Liebermann H (1966): Isolation of a bovine herpesvirus from calves with respiratory disease and keratoconjunctivitis. Acta Vet Acad Sci Hung, 16, 357-358.
2. Belak S, Palfi V (1974): Characterization of a herpesvirus isolated from spontaneously degenerated bovine kidney cell culture. Acta Vet Acad Sci Hung, 24, 249-253.
3. Bilge-Dagalp S, Demir B, Güngör E, Alkan F (2007): The seroprevalence of bovine herpesvirus type 4 (BoHV-4) infection with reproductive disorders. Rev Med Vet, 158, 201-205.
4. Czaplicki G, Thiry E (1998): An association exists between bovine herpesvirus 4 seropositivity and abortion in cows. Prev Vet Med, 33, 235-240.
5. Dubuisson J, Thiry E, Bublot M, Thomas I, Van Bressem MF, Coignoil F, Pastoret PP (1989): Experimental infection of bulls with a genital isolate of bovine herpesvirus 4 and reactivation of latent virus with dexamethasone. Vet Microbiol, 21, 97-114.
6. Fabian K, Makrai L, Sachse K, Szeredi L, Egyed L (2008): An investigation of the aetiological role of bovine herpesvirus 4 in bovine endometritis. Vet J, 177, 289-292.
7. Frazier K, Baldwin C, Pence M, West J, Bernard J, Liggett A, Miller D, Hines M (2002): Seroprevalence and comparison of isolates of endometriotropic bovine herpesvirus-4. J Vet Diag Invest, 14, 457-462.
8. Goltz M, Broll H, Mankertz A, Weigelt W, Ludwig H, Buhk HJ, Borchers K (1994): Glycoprotein B of bovine herpesvirus type 4: Its phylogenetic relationship to gB equivalents of the herpesviruses. Virus Genes, 9, 53-59.

9. Little SP, Jofre JT, Courtney RJ, Schaffer PA (1981): A virion –associated glycoprotein essential for infectivity of herpes simplex virus type 1. Virology, 115, 149-160.
10. Lomonte P, Filee P, Lyaku JR, Bublot M, Pastoret PP, Thiry E (1997): Glycoprotein B of bovine herpes- virus 4 is a major component of the virion, unlike that of two other gammaherpesviruses, Epstein Barr virus and murine gammaherpesvirus 68. J Virol, 71, 3332-3335.
11. Miyano H, Haritani M, Sentsui H, Tsuboi T, Tanimura N, Kimura KK, Kobayashi M, Obara N, Akimoto Y (2004): Mammary lesions associated with bovine herpesvirus Type 4 in a cow with clinical mastitis. J Vet Med Sci, 66, 457-460.
12. Monge A, Elvira L, Gonzalez JV, Astiz S, Wellenberg GJ (2006): Bovine herpesvirus 4-associated postpartum metritis in a Spanish dairy herd. Res Vet Sci, 1, 120-125.
13. Nettleton PF, Herring JA, Herring AJ (1983): Evaluation of an immunofluorescent test for the rapid diagnosis of field infections bovine rhinotracheitis. Vet Rec, 112, 298-300.
14. Nikolin VM, Donofrio G, Milosevic B, Taddei S, Radosavljevic V, Milicevic V (2007): First Serbian isolates of bovine herpesvirus 4 (BoHV 4) from a herd with a history of postpartum metritis. New Microbiol, 30, 53- 57.
15. Osorio FA, Reed DE (1983): Experimental inoculation of cattle with BoHV-4 evidence for a lymphoid associated persistent infection. Am J Vet Res, 44, 975-980.
16. Roizman B, Desroisers RC, Fleckenstein B, Lopez C, Minson AC, Studdert MJ (1992): The family herpesviridae: an update. Arch Virol, 123, 425-449.
17. Sambrook J, Fritsch EF, Maniatis T (1989): Molecular cloning. In: Sambrook J, Fritsch EF, Maniatis T, Eds. A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.
18. Wellemans G, Van Opdenbosch E, Mammerickx M (1986): Experimental inoculation of bovine herpesvirus 4 (strain LVR 140) in pregnant and non-pregnant cows. Ann Rech Vet, 17, 89-94.
19. Wellenberg GJ, Van Der Poel WHM, Van Der Vorst TJK, Van Valkengoed PHR, Schuhken YH, Wagenaar F, Van Oirschot JT (2000): BoHV-4 in bovine clinical mastitis. Vet Rec, 147, 222-225.
20. Wellenberg GJ, Verstraten ERAM, Belak S, Verschuren FAM, Rijsewijk R, Peshev R, Van Oirschot JT (2001): Detection of bovine herpes virus 4 glycoprotein B and thymidine kinase DNA by PCR assays in bovine milk. J Virol Methods, 97, 101-112.