The comparison of agglutination, hemagglutination inhibition and indirect hemagglutination tests inthe serological diagnosis ofinfectious coryza in chickens

Abstract

This study was carried out to investigate the feasibility of agglutination, hemagglutination inhibition (HI) and indirect hemagglutination (IHA) tests in the diagnosis of Infectious Coryza in chickens and to compare these tests. The agglutination tests were performed by using both serotype specific plate agglutination test antigens (serotype A, B and C specific) and the whole cell plate agglutination test antigen that prepared from serotype C (Modesto) strain. 510 sera were tested in this study. 42.75% of the field sera were found positive and 57.25% were found negative in plate agglutination tests performed with whole cell antigen. The test sera of 31.37% were found positive in regards to have serotype A antibodies and 15.1% of them were found positive in regards to have serotype C antibodies and remaining 53.53% were found to be negative in plate agglutination test performed with serotype spesific plate agglutination antigens. Hemagglutination did not occur in hemagglutination test performed with fresh chicken erythrocytes. Whereas in hemagglutination test which was performed with glutaraldehyde-fixed chicken erythrocytes, 1/64 HA titer was determined. Consequently, while the agglutination tests performed with whole cell antigen was found to be sensitive, HI and IHA were inefficient in the detection of seropositive chickens. Plate agglutination test performed with serotype spesific antigens were found to be useful to determine chickens infected with these serotype strains

Keywords

• Infectious coryza
• serological diagnosis

Tavuklarda infeksiyöz koriza’nın serolojik teşhisinde aglutinasyon, hemaglutinasyon inhibisyon ve indirekt hemaglutinasyon testlerinin karşılaştırılması

Abstract

Bu çalışma, tavuklarda İnfeksiyöz Koriza’nın serolojik teşhisinde aglutinasyon, hemaglutinasyon inhibisyon (HI) ve indirekt hemaglutinasyon (IHA) testlerinin kullanılabilirliğini araştırmak ve bu testler arasında bir karşılaştırma yapmak amacıyla yapıldı. Aglutinasyon testi hem serotip spesifik lam aglutinasyon antijenleri (serotip A, B ve C spesifik) hem de serotip C (Modesto) suşundan hazırlanan tüm hücre lam aglutinasyon antijeni kullanılarak yapıldı. Çalışma kapsamında 510 serum incelendi. Tüm hücre antijeni ile yapılan lam aglutinasyon testinde saha serumlarının %42.75’i pozitif, %57.25’i ise negatif sonuç verdi. Serotip spesifik lam aglutinasyon antijenleri ile yapılan testte, serumların %31.37’si serotip A, %15.1’i serotip C antikorları yönünden pozitif bulundu. Serumların %53.53’ü ise sero-negatif bulundu. Taze tavuk eritrositleri ile yapılan hemaglutinasyon testinde aglutinasyon görülmezken; gluteraldehit ile fikse edilmiş tavuk eritrositlerinin kullanıldığı hemaglutinasyon testinde 1/64 titre belirlendi. Sonuç olarak, seropozitif tavukların saptanmasında tüm hücre antijeni ile yapılan aglütinasyon testinin duyarlı olduğu belirlenirken, HI ve IHA testlerinin yetersiz kaldığı saptandı. Serotip spesifik antijenlerle yapılan lam aglutinasyon testinin de infekte hayvanları belirlemede yararlı olduğu görüldü

Keywords

• infeksiyoz koriza
• serolojik teşhis

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