Localization of insulin immunpositive cells and histochemical structure of the pancreas in falcons (Falco Anaumanni)

Abstract

The present study was conducted to determine the regional distribution of insulin immunpositive cells and histochemical structure of pancreas in falcon (Falco Anaumanni). Falcon pancreas was composed of three different parts as dorsal, ventral and splenic lobes. Those lobes contained acinar cells, pancreatic ducts, alpha, beta and mixed islets. Alpha islets were determined 3-4 unit as large islets in centre of the splenic lobe, but a few as small islets in dorsal and ventral lobes. Beta islets were widespread as small clusters in all lobes which were especially 7-8 units in periphery of the splenic lobe. Furthermore, mixed islets were found abundantly as small or large islets in all lobes. In the streptavidin biotin peroxidase staining of the pancreatic lobes, insulin immunpositive cells were numerous in the centre of beta islets, on the other hand these cells were detected as only one cell or 3-8 immunpositive cell groups in both alpha islets and exocrine tissue. In conclusion, the morphology and insulin immunpositive cells of pancreas in falcons are similar to duck, whereas are different from other avian species such as chicken, goose, and quail

Keywords

• Falcon
• immunohistochemistry
• insulin immunpositive cells
• pancreas

Şahinlerde (Falco Anaumanni) pankreasın histokimyasal yapısı ve insülin immunpozitif hücrelerinlokalizasyonu

Abstract

Bu çalışma, şahin (Falco Anaumanni) pankreasının histokimyasal yapısı ve insülin immunpozitif hücrelerin bölgesel dağılımını belirlemek için yapıldı. Şahin pankreası dorsal, ventral ve dalak lobu olarak 3 farklı lobdan oluşuyordu. Bu loplar asiner hücreler, akıtıcı kanallar, alfa, beta ve mix adacıkları içermekteydi. Alfa adacıkları dalak lobunun merkezinde 3-4 adet büyük adacık halinde gözlenirken, dorsal ve ventral lopta az sayıda küçük adacıklar halinde belirlendi. Beta adacıkları özellikle dalak lobunun periferinde 7-8 adet küçük adacıklar halinde bütün loplarda çok sayıda tespit edildi. Ayrıca, miks adacıklarının küçük ya da büyük adacıklar halinde pankreasın bütün loplarında çok yaygın bir şekilde bulunduğu saptandı. Pankreatik lobların streptavidin-biotin peroksidaz boyamalarında, insulin immunpozitif hücreler beta adacıkları merkezinde çok sayıdaydı diğer yandan bu hücreler hem alfa adacıklarında hem de ekzokrin dokuda yalnız tek bir hücre ya da 3-8 hücrelik gruplar halinde görüldü. Sonuç olarak şahin pankreasının morfolojik ve insulin pozitif hücreler açısından ördek pankreası ile benzer olduğu, tavuk, kaz ve bıldırcın gibi diğer kanatlı türlerinin pankreaslarından farklı olduğu tespit edilmiştir

Keywords

• İmmunhistokimya
• insulin pozitif hücreler
• pancreas
• şahin

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