Determined of phytochemical properties of sainfoin (Onobrychis viciifolia) and acute orally LD50 in mice

Abstract

This in vitro study was aimed to determine the phytochemical properties and acute LD50 of oral administration of the extract of sainfoin (Onobrychis viciifolia) which is largely distributed in Turkey. The phytochemical properties of the sainfoin extract were determined presence of alkaloids, saponins, tannins, peptide/proteins, sugars, amino acids, phenols and phenolic acid, choline and flavonoids. Sixty female and 60 male of total 120 numbers of wistar albino mice, 25-35g weight, approximately two months age were used to determine acute LD50 level of the sainfoin extract. Acute oral LD50 of sainfoin was found ≥19000 mg/kg for males and ≥20000 mg/kg for females and phytochemical analysis of the extract or plant showed the presence of tannins, amino acids, proteins, sucrose, phenolic contents; but the presence of alkaloids, simple sugars, saponins and choline were not observed. We concluded that the acute toxicity of the plant orally administered to mice was low, which suggested that animal can consume safely it

Keywords

• LD50
• mice
• phytochemical properties
• sainfoin

Korunga bitkisinin (Onobrychis viciifolia) fitokimyasal içeriği ve farelerde akut oral ÖD50’sinin belirlenmesi

Abstract

Bu araştırmada, Türkiye’de yaygın bir şekilde yetiştirilen korunga bitkisinin (Onobrychis viciifolia) fitokimyasal içeriği ile ekstraktının farelerde ağızdan akut ÖD50 miktarının belirlenmesi amaçlanmıştır. Korunga bitkisinin kimyasal incelemesinde; alkaloidlerin, saponinlerin, tanenlerin, peptid/proteinlerin, şekerlerin, aminoasitlerin, fenoller ve fenolik asitlerin, kolin ve flavonoidlerin varlığı araştırıldı. Akut ÖD50 miktarının belirlenmesi için 25–35 g ağırlığında, yaklaşık 2 aylık Wistar Albino soyu, 60 dişi ve 60 erkek toplam 120 adet fare kullanıldı. Farelerde ağızdan akut ÖD50 değerinin erkeklerde ≥19000 ve dişilerde ≥20000 mg/kg olduğu ve bitkinin veya ekstraktının fitokimyasal incelemesinde tanen, aminoasit, protein, sukroz, fenol ve flavonoidlerin olduğu, alkaloid, basit şeker, saponin ve kolin varlığının olmadığı gözlendi. Bitkinin farelerdeki ağızdan akut zehirliliğinin düşük olduğu ve hayvanlar tarafından güvenle tüketilebileceği sonucuna varılmıştır

Keywords

• Fare
• fitokimyasal içerik
• korunga
• ÖD50

References

1. AOAC (1990). Official Methods of Analysis of the Association of Official Analytical Chemists. 14th Ed., Virginia, USA.
2. Açıkgöz E (2001): Yem Bitkileri. 1–95. 3. Baskı. Vipaş A.Ş. Bursa. ISBN: 975–564–124–6.
3. Anon (2006): Proteinlerin saflaştırılması. Erişim: http://yunus.hacettepe.edu.tr /~umut/lab_ pdf/408. pdf Erişim Tarihi: 08.12.2006.
4. Barrau E, Fabre N, Fouraste I, Hoste H (2005): Effect of bioactive compounds from sainfoin (Onobrychis viciifolia Scop.) on the in vitro larval migration of Haemonchus contortus: role of tannins and flavonol glycosides. J Parasitol, 131, 1–8.
5. Doull J, Klaassen CD, Amdur OM (1975): Casarett and Doull’s Toxicology: the basic science of poisions. 2th ed., Macmillan, New york.
6. Duke JA (1983): Handbook of energy crops. (unpublished). Erişim: http://www.hort.purdue.edu/newcrop/duke_energy/Vicia_ faba.html.
7. Hapner KD, Robbins JE (1979): Isolation and properties of lectin from sainfoin (Onobrychis viciifolia , Scop.). BBA, 580, 186–197.
8. Harborne JB (1973): Phytochemical Metods: A guide to modern techniques of plant analysis. Chapman and Hall. London.

9. Heckendorn F, Häring DA, Maurer V, Zinsstag J, Langhans W, Hertzberg H (2006): Effect of sainfoin (Onobrychis viciifolia) silage and hay on established populations of Haemonchus contortus and Cooperia curticei in lambs. Vet Parasitol, 142, 293–300.
10. Hoste H, Gaillard L, Frileux LY (2005): Consequences of the regular distribution of sainfoin hay on gastrointestinal parasitism with nematodes and milk production in dairy goats. Small Ruminant Res, 59, 265–271.
11. Inchem (1971): Toxicological evaluation of some extraction solvents and certain other substances. FAO Nutrition Meetings Report Series No. 48A WHO/FOOD ADD/70.39. Erişim: http://www.inchem.org/documents/jecfa/jecmono/v48aje 14.htm Erişim tarihi: 28.12.2006.
12. Kaya S (2002): Doz ve etki ilişkisi. 47-48. Alınmıştır: (Ed) Kaya S, Pirinçci İ, Bilgili A. Veteriner Hekimliğinde Toksikoloji. Medisan Yayınevi. Ankara
13. Kaya S (2006): Zehirli Maddelerin Laboratuar Analizi. 56-70. Medisan Yayınevi. Ankara.
14. Kaya S, Bilgili A (2002): Zehirlenme ve zehirlilik denemeleri. 22. Alınmıştır: (Ed) Kaya S, Pirinçci İ, Bilgili A. Veteriner Hekimliğinde Toksikoloji. Medisan Yayınevi. Ankara
15. Lennon RE, Hunn JB, Schnick RA (1971): Reclamation of ponds, lakes and streams with fish toxicants: a review. FAO Fisheries Technical Papers T100. Erişim: http://www.fao.org/docrep/003/b0465e/B0465E09.htm#CH9.2 - T100. Erişim:
16. Lu Y, Sun Y, Foo LY, Mcnabb WC, Molan AL (2000): Phenolic glycosides of forage legume Onobrychis viciifolia. Phytochemistry, 55, 67–75.
17. Lutterodt GD (1988): Responses of gastrointestinal smooth mucle preparations to a muscarinic principle present in Sida veronicaefolia. J Ethnopharmacol, 23, 313– 322.
18. Marais JPJ, Mueuller-Harvey I, Brandt EV, Ferreira D (2000): Polyphenols, condansed tannins and other natural products in Onobrychis viciifolia (Sainfoin). J Agric Food Chem, 48, 3440–3447.
19. Marti D, Villagrasa V, Martinez-Solis I, Blanquer A,
20. Castillo E, Moreno LR
21. (2005): Hystological and
22. pharmacological study of Thymus piperella (L.). Phytother Res, 19, 298–302.
23. Namen AE, Hapner KD (1979): The glycosyl moiety of lectin from sainfoin (Onobrychis viciifolia Scop.). BBA, 580, 198–209.
24. Paolini V, Fouraste I, Hoste H (2004): In vitro effects of three woody plant and sainfoin extracts on 3rd-stage larvae and adult worms of three gastrointestinal nematodes. J Parasitol, 129, 69–77.
25. Paolini V, Prevot F, Dorchies PH, Hoste H (2005): Lack of effects of quebracho and sainfoin hay on incoming third-stage larvae of Haemonchus contortus in goats. Vet J, 170, 260–263.
26. SPSS (2002): Statistical Package for the Social Sciences. SPSS for Windows 11,5. serial no: 9024147.