Light and electron microscopic studies on the oviduct epithelium of the Pekin duck (Anas platyrhynchos)

Abstract

The present study was undertaken to investigate the histochemical properties and light and electron microscopic morphology of the oviduct of laying Pekin ducks and Pekin ducks in the quiescent phase of the reproductive cycle. The oviducts of five healthy, two-year-old adult laying Pekin ducks and five five-month-old Pekin ducks in the quiescent phase of the reproductive cycle, obtained from the Farm of Ankara University Faculty of Veterinary Medicine, constituted the material of the present study. The oviduct of the Pekin duck was segmented into five regions, namely, the infundibulum, magnum, isthmus, uterus and vagina. Along the oviduct, the tunica mucosa forms convolutions protruding into the lumen, and the height of these convolutions were determined to increase in the regions of the magnum and uterus. The lamina epithelialis was ascertained to be composed of ciliated and secretory cells. The lamina propria was filled with glands in the magnum, isthmus and uterus. In laying ducks, the PAS-positive reaction in the isthmus and the performic acid/Alcian blue pH 2.5 reaction in the magnum were determined to be very strong. On the other hand, due to the cells forming the lamina epithelialis and the proprial glands not having developed fully, these reactions were observed to be weak in ducks in the quiescent phase of the reproductive cycle. In electron microscopic examinations, a single type of secretion granule, of medium electron density and varying size, was observed in the secretory cells of the lamina epithelialis. Electron dense secretion granules were present in the proprial glands of the magnum and isthmus, whereas proprial glands of the uterus contained electron light granules

Keywords

• Histochemistry
• oviduct
• Pekin duck

Pekin ördeği (Anas platyrhynchos) ovidukt epitelinde ışık ve elektron mikroskobik çalışmalar

Abstract

Bu çalışma, yumurtlayan ve yumurtlamaya başlamamış Pekin ördeği ovidukt’unun histokimyasal özelliklerini, ışık ve elektron mikroskobik morfolojisini incelemek amacıyla yapıldı. Çalışmada materyal olarak Ankara Üniversitesi Veteriner Fakültesi çiftliğinden sağlanan 5’er adet sağlıklı, yumurtlayan (erişkin, 2 yaşında ) ve yumurtlamaya başlamamış (5 aylık) Pekin ördeği oviduktu kullanıldı. Pekin ördeği oviduktu; infundibulum, magnum, istmus, uterus ve vagina olmak üzere beş bölgeden oluşmuştu. Oviduktta tunika mukozanın lümene doğru kıvrımlar yaptığı ve bu kıvrımların yüksekliğinin magnum ve uterusda arttığı gözlendi. Lamina epitelyalisin silyumlu ve sekretorik hücrelerden oluştuğu görüldü. Magnum, istmus ve uterusda lamina propriya bezlerle doluydu. Yumurtlayan ördeklerde istmus bölgesinde PAS(+) reaksiyonun, magnum bölgesinde performik asit/Alcian blue pH 2.5 reaksiyonunun çok kuvvetli olduğu görüldü. Yumurtlamayanlarda ise lamina epitelyalisi oluşturan hücrelerin ve lamina propriyadaki bezlerin henüz gelişmediği ve buna bağlı olarak reaksiyonların zayıf olduğu izlendi. Elektron mikroskopik incelemelerde, lamina epitelyalisdeki sekretorik hücrelerde değişik büyüklükte tek tip, orta yoğunlukta salgı granülleri gözlendi. Magnum ve istmus bölgelerinin lamina propriyasındaki bezlerde elektron koyu salgı granülleri görülürken, uterus bölgesindeki bezlerde ise elektron açık granüller görüldü

Keywords

• Histokimya
• ovidukt
• Pekin ördeği

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