Taylarda Rhodococcus equi enfeksiyonunun patolojik bulguları ve immunoperoksidaz tekniği ile tanısı

Abstract

Klinik olarak solunum güçlüğü bulguları gösteren ve 2003-2006 yıllarının ilkbahar-yaz aylarında ölen 12 tayın nekropsisi yapıldı. On iki tayda da R. equi’ye bağlı pnömoni saptandı. Makroskobik olarak; 5 olguda akciğerde tipik kazeonekrotik odaklarla karşılaşıldı. Histopatolojik incelemede; irinli bronkopnömoni tablosu dikkati çekti. R. equi’ nin, yapılan immunoperoksidaz boyamalarla, akciğer ve mediastinal lenf düğümü kesitlerinde, çoğunlukla makrofajların, bazen de nötrofil lökositlerin sitoplazmasında yerleştiği görüldü. Bağırsaklarda lezyon görülmemesi, bu formun akciğer formuna göre ender şekillendiğini gösterdi. Sadece iki olguda mikrobiyolojik olarak R. equi izole edilebildi. Sonuç olarak; çalışmada kullanılan 12 tayda da R. equi’nin saptanmış olması, taylarda görülen pnömonilerin çoğunluğundan bu bakterinin sorumlu olduğunu ve ayrıca tanısında immunoperoksidaz tekniğinin başarıyla kullanılabileceğini gösterdi

Keywords

• İmmunoperoksidaz tekniği
• patoloji
• Rhodococcus equi
• tay

The pathological findings of Rhodococcus equi infection and its diagnosis with immunoperoxidase technique in foals

Abstract

Twelve foals with difficulty in respiration that have died during spring-summer months between 2003-2006 years were necropsied. On necropsy, pneumonia was detected in all of the foals. In 5 animals, caseo-necrotic nodules were seen in the lungs. In histopathological examination, purulent bronchopneumonia was noticed. In immunohistochemical staining, Rhodococus equi antigens were determined in the cytoplasm of macrophages and occasionally in neutrophils in lungs and mediastinal lymph nodes by immunoperoxidase technique. No lesions were detected in the intestines confirming that this form of infection is rare compared to the lung form. In the present study, R. equi was microbiologically isolated only in two cases. In conclusion, R. equi infection was diagnosed in all of the twelve cases, and estimated to be the reason of pneumonia in the foals. It was also shown that immunoperoxidase technique can be successfully used for R. equi infection in the field

Keywords

• Foal
• immunoperoxidase technique
• pathology
• Rhodococcus equi

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