Decreased serum sialic acid, albumin-globulin ratio and total protein levels in cattle heavily infected with Theileria annulata

Abstract

The present study was designed to determine the changes in the levels of serum total sialic acid and total protein, albumin/globulin ratio in naturally infected cattle with tropical theileriosis for a better understanding of the pathogenesis of tropical theileriosis. Eight cattle heavily infected (50-70% parasitamia) with Theileria annulata had significantly lower serum sialic acid, total protein, albumin and globulin levels and albumin-globulin ratios than uninfected eight control cattle. Levels of serum sialic acids were found to be 133.87±0.77 mg/dl and 194.12±3.91 mg/dl in cattle with theileriosis and healthy animals respectively. These values between two groups found to be significant (p<0.001). Levels of serum total protein were found to be 5.70 ± 0.17 g/dl and 7.00 ± 0.14 g/dl in cattle with theileriosis and healthy animals respectively. These values between two groups found to be statistically significant (p<0.001). Levels of serum albumin were found to be 2.16 ± 0.04 g/dl and 3.02 ± 0.04 g/dl in cattle with theileriosis and healthy animals respectively. These values between two groups found to be statistically significant (p<0.00). Levels of serum globulin were found to be 3.53 ± 0.15 g/dl and 3.97 ± 0.12 g/dl in cattle with theileriosis and healthy animals respectively. These values between two groups found to be statistically significant (p<0.05). Albumin-globulin ratio in serum were found to be 0.61 ± 0.02 and 0.76 ± 0.02 in cattle with theileriosis and healthy animals respectively. These values between two groups found to be statistically significant (p<0.002). These findings suggest that levels of serum proteins are usually decreased significantly in Tropical theileriosis, which may be the result of liver damage. In paralel to decreased serum proteins, sialisation of proteins in tropical theileriosis may be the result of decreased glycoproteins during the disease process. These results may be useful to evaluate the prognosis and treatment of the disease with the other laboratory findings in clinic

Keywords

• Sialic acid
• total protein
• albumin:globulin ratio
• cattle
• Theileria annulata

Theileria annulata ile yüksek oranda enfekte sığırlarda azalan serum sialik asit, albumin-globulinoranı ve toplam protein düzeyleri

Abstract

Çalışmada, sığırlarda tropikal theileriosis’in patogenezis yönünden daha iyi anlaşılabilmesi için serum sialik asit, toplam protein, albumin ve globulin düzeyleri ile albumin-globulin oranları araştırılmıştır. Enfekte olmayan (kontrol) sekiz sığır ile karşılaştırıldığında, Theileria annulata ile yüksek oranda enfekte (%50-70 parazitemi) olan sekiz hasta sığırda serum sialik asit, toplam protein, albumin ve globulin düzeyleri ile albumin-globulin oranlarının önemli derecede düştüğü belirlenmiştir. Serum sialik asit ortalama değerleri sağlıklı sığırlarda 194.12 ± 3.91 mg/dl, hasta sığırlarda ise 133.87 ± 0.77 mg/dl olarak tespit edilmiş, gruplar arası farklılık önemli bulunmuştur (p<0.001). Serum toplam protein ortalama değerleri sağlıklı olanlarda 7.00 ± 0.14 g/dl, hastalarda ise 5.70 ± 0.17 g/dl olarak tespit edilmiş, gruplar arası farklılık önemli bulunmuştur (p<0.001). Serum albumin ortalama değeri ise sağlıklı ve hastalıklı olanlarda sırasıyla 3.02 ± 0.04 ve 2.16 ± 0.04 g/dl hesaplanmış ve farkın istatistik önemli olduğu saptanmıştır (p<0.000). Serum globulin ortalamaları sırasıyla sağlıklı olanlarda 3.97 ± 0.12 g/dl, hastalarda 3.53 ± 0.15 g /dl olarak tespit edilmiş, gruplar arası farklılığın istatistik önemli olduğu hesaplanmıştır (p<0.05). Serum albumin-globulin oranı ortalaması sağlıklı grupta 0.76 ± 0.02, hastalıklı grupta ise 0.61 ± 0.02 olarak bulunmuş ve farkın istatistik önemli olduğu tesbit edilmiştir (p<0.002). Bu sonuçlar, serum proteinlerinin önemli derecede azaldığını göstermektedir. Serum proteinlerindeki bu azalış karaciğer hasarının bir sonucu olabilir. Önemli derecede azalan protein düzeylerine paralel olarak, tropikal theileriosis’de proteinlere bağlı düşük sialik asit düzeyleri de hastalığın gelişimi süresince glikoproteinlerdeki azalışın bir sonucu olabilir. Bu sonuçların hastalığın prognoz ve tedavisinin izlenmesinde klinikteki diğer laboratuvar bulguları ile birlikte değerlendirilmesinin faydalı olabileceği kanaatine varılmıştır

Keywords

• sialic acid
• toplam protein
• albumin:globulin oranı
• sığır
• Theileria annulata

References

1. Altintas A, Kurtdede A, Fidanci UR, Borku MK (1989): Köpek gençlik hastalığında (Distember) serum sialik asit ve protein düzeylerinin klinik önemi. Ankara Üniv Vet Fak Derg, 36, 154-164.
2. Bhattacharyuly Y, Chaudhri R, Gill B (1975): Transstadial transmission of Theileria annulata through common ixodid ticks infesting Indian cattle. Parasitology, 71, 1-7.
3. Brown CGD (1990): Control of tropical theileriosis (Theileria annulata infection) of cattle. Parasitologia, 32, 23-31.
4. Citil M, Gunes, V, Karapehlivan M, Atalan G, Maraslı S (2004): Evaluation of serum sialic acid as an inflammation marker in cattle with traumatic reticulo peritonitis. Rev Med Vet, 155, 389-392.
5. Corfield AP, Schauer R (1982): Sialic Acids. Springer- Verlag, Wien, New York.
6. Dolan TT (1989): Theileriosis: a comprehensive review. Rev Sci Tech Off I E, 8, 11-36.
7. Doumas BT, Watson WA, Biggs HG (1971): Albumin standards and the measurement of serum albumin with bromcresol green. Clin Chim Acta, 31, 87-96.
8. Dumanlı N, Aktas M, Cetinkaya B, Cakmak A, Koroglu E, Sakı CE, Erdogmus Z, Nalbantoglu S, Ongor H, Simsek S, Karahan M, Altay K (2005): Prevalance and distribution of tropical theileriosis in eastern Turkey. Vet Parasitol, 127, 9-15.

9. Ekin S, Mert N, Gunduz H, Meral I (2003): Serum sialic acid levels and selected mineral status in patients with type 2 diabetes mellitus. Biol Trace Elem Res, 94, 193-201.
10. Eren H, Cakmak A, Yukarı BA (1995): Türkiye’nin farklı cografik bölgelerinde Theileria annulata’nın seroprevalansı. Ankara Üniv Vet Fak Derg, 42, 57-60.
11. Hemming FW (1991): Glycoproteins of Mammalian and Avian Cells. 217-253. In Post-translational Modifications of Proteins. 1st Edn. Eds. J.J.Harding and M.J.C. Crabbe. CRC Pres, London.
12. Kaneko JJ (1997): Serum Proteins and Dysproteinemias. 117-138. In Clinical Biochemistry of Domestic Animals. 5th Edn. Eds. J.J. Kaneko, J.W. Harwey, M.L., Academic Press, Bruss. San Diego.
13. Karagenç TI, Kıral FK, Seyrek K, Bildik A, Eren H (2005): Detection of serum total sialic acid in cattle with natural tropical theileriosis. Revue Med Vet, 156, 11, 578- 582.
14. Kloppel TM, Franz CP, Morre DJ, Richardson RC (1978): Serum sialic acid levels increased in tumor- bearing dogs. Am J Vet Res, 39, 1377-1380.
15. Laiblin C, Baysu N, Muller M (1978): Clinical study on experimental Theileria annulata infections of cattle. 1. Clinical chemical study. Berl Munc Tierarztl Wochenschr, 91, 25-27.
16. Makimura S, Usui M (1990): Correlation between haptoglobulin and sialic acid or mucoprotein in diseased bovine serum. Jpn J Vet Sci, 52, 1245-50.
17. Ohnishi ST, Barr JK (1978): A simplified method of quantitating protein using the biuret and phenol reagent. Anal Biochem, 86, 193-200.
18. Omer OH, El-Malik KH, Magzoub M, Mahmoud OM, Haroun EM, Hawas A, Omar HM (2003): Biochemical profiles in friesian cattle naturally infected with Theileria annulata in Saudi Arabia. Vet Res Commun, 27, 15-25.
19. Pipano E, Shkap V (2000): Vaccination against tropical theileriosis. Ann N Y Acad Sci, 916, 484-500.
20. Purnell RE (1978): Theileria annulata as a hazard to cattle in the countries on the Northern Mediterranean littoral. Vet Sci Commun, 2, 3-10.
21. Sandhu GS, Grewal AS, Sing A, Kondal JK, Singh J, Brar RS (1998): Haematological and biochemical studies on experimental Theileria annulata infection in crossbred calves. Vet Res Commun, 22, 347-354.
22. Sayin F, Dinçer S, Karaer Z, Cakmak A, Inci A, Yukarı BA, Eren H., Vatansever Z, Nalbantoglu S (2002): Studies on the epidemiology of Tropical theileriosis (Theileria annulata infection) in cattle in Central Anatolia. Trop Anim Health Pro, 34, 1-18.
23. Singh A, Singh J, Grewal AS, Brar RS (2001): Studies on some blood parameters of crossbred calves with experimental Theileria annulata infections. Vet Res Commun, 25, 289-300.
24. Sydow G (1985): A simplified quick method for determination of sialic acid in serum. Biomed Biochim Acta, 44, 1721-1723.
25. Yadav CL, Sharma NN (1986): Changes in blood components during experimentally induced Theileria annulata infection in cattle. Vet Parasitol, 21, 91-98.