Comparison of qualitative and quantitative alterations caused by use of various fixatives in the myocardium

Abstract

In the field of anatomy, preservation techniques used to preserve the integrity of specimens prepared for use as educational and research material are very ancient. It is desirable that these materials be as close as possible to the characteristics of living animal tissues in order to be an effective educational material. One of the most important criteria in determining similarity to fresh cadavers is the color and odor changes in the tissues. Therefore, the aim of this study is to reveal the anatomical and physiological changes on myocardial tissue, fixed by 4 different solutions in which qualitative and quantitative methods such as color analysis, sensory analysis, and microbiological tests. As a result, it was determined that a newly developed enriched saline solution and low concentration formaldehyde solution can be used safely for the preparation, preserve and long term use of educational materials.

Keywords

• Colorimeter,fixation,sensory analysis,solution

References

1. 1. Ajayi IE, Shawulu JC, Ghaji A, et al (2011): Use of formalin and modified gravity-feed embalming technique in veterinary anatomy dissection and practicals. J Vet Med Anim Health, 3, 79-81.
2. 2. Al Aiyan A, Weigner J, Lübke-Becker A, et al (2012): Long term study on microbial status in anatomical cadaver fixation using reduced formalin concentration. BJVM, 15, 74.
3. 3. Bakici C, Akgün RO, Ekim O, et al (2017): Is kaiserling solution a convenient fixative for mammalian organ specimens? Evaluation of morphometric, colorimetric and volumetric properties. BJVM, 20, 62-67.
4. 4. Batra APS, Khurana BS, Mahajan A, et al (2010): Embalming and other methods of dead body preservation. IJMTFM, 12, 12-19.
5. 5. Bendino JH (2003): Embalming chemistry: glutaraldehyde versus formaldehyde. Avaliable at https://pdfs.semanticscholar.org/3fb1/19f2bd6276f76522b192a63077a5f6019f68.pdf (Accessed October 10, 2017).
6. 6. Brenner E (2014): Human body preservation – old and new techniques. J Anat, 224, 316-344.
7. 7. Fricker J, Zeiler E, McDaniel BJ (2007): From formalin to salt. Development and introduction on a salt-based preserving solution for macroscopic anatomic specimens. Tierarztl Prax Ausg K Kleintiere Heimtiere, 35, 243-248.
8. 8. Hammer N, Löffler S, Bechmann I, et al (2015): Comparison of modified thiel embalming and ethanol-glycerin fixation in an anatomy environment: potentials and limitations of two complementary techniques. Ana Sci Educ, 8, 74-85.

9. 9. Inglez DS, Matera JM (2015): Bleeding simulation in embalmed cadavers: bridging the gap between simulation and live surgery. ALTEX, 32, 59-63.
10. 10. Janczyk P, Weigner J, Luebke-Becker A, et al (2011a): Nitrite pickling salt as an alternative to formaldehyde for embalming in veterinary anatomy - A study based on histo- and microbiological analyses. Ann Anat, 193, 71-75.
11. 11. Janczyk P, Weigner J, Luebke-Becker A, et al (2011b): A pilot study on ethanol-polyethylene glycol-formalin fixation of farm animal cadavers. Berl Münch Tierärztl Wochenschr, 124, 225-227.
12. 12. Jangde S, Arya RS, Paikra S, et al (2015): How to provide a safe working condition for medical students and professionals in the anatomy dissection room. Sch J App Med Sci, 3, 1867-1870.
13. 13. Kays SJ (1999): Preharvest factors affecting appearance. Postharvest Biol Technol, 15, 233-247.
14. 14. Leon K, Mery D, Pedreschi F, et al (2006): Color measurement in L*a*b* units from RGB digital images. Food Res Int, 39, 1084-1091.
15. 15. MacDonald GJ, MacGregor DB (1997): Procedures for embalming cadavers for the dissecting laboratory. Proc Soc Exp Biol Med, 215, 363-365.
16. 16. Meilgaard M, Civille GV, Carr T (1999): Sensory evaluation technique. CRC Press, USA.
17. 17. O’neill GJ, Pais D, de Andrade FF, et al (2013): Improvement of the embalming perfusion method: The innovation and the results by light and scanning electron microscopy. Acta Med Port, 26, 188-194.
18. 18. Oliveria AC, Balaban MO (2006): Comparison of a colorimeter with a machine vision system in measuring color of Gulf of Mexico sturgeon fillets. Appl Eng Agric, 22, 583-587.
19. 19. Persnell SE (2015): Postmortem changes. Available at https://emedicine.medscape.com/article/1680032-overview (Accessed May 10, 2017).
20. 20. Plymale DR, Tang DS, Comardelle AM, et al (1999): Both necrosis and apoptosis contribute to HIV-1 induced killing of CD4 cells. AIDS 13, 1827-1839.
21. 21. Rai BP, Tang B, Eisma R (2012): A qualitative assessment of human cadavers embalmed by Thiel’s method used in laparoscopic training for renal resection. Anat Sci Educ, 5, 182-186.
22. 22. Ryan EM, Duryee MJ, Hollins A (2019): Antioxidant properties of citric acid interfere with the uricase-based measurement of circulating uric acid. J Pharm Biomed Anal, 164, 460-466.
23. 23. Rostamzad H, Shabanpour B, Kashaninejad M, et al (2011): Antioxidative activity of citric and ascorbic acids and their preventive effect on lipid oxidation in frozen persian sturgeon fillets. Lat Am Appl Res, 41, 135-140.
24. 24. Saeed M, Rufai AA, ElSayed SE et al (2001): Mummification to plastination. Saudi Med J, 22, 956-959.
25. 25. Silva RMG, Matera JM, Ribeiro AACM (2004): Preservation of cadavers for surgical technique training. Vet Surg, 33, 606-608.
26. 26. Silva RMG, Matera JM, Ribeiro AACM (2007): New alternative methods to teach surgical techniques for veterinary medicine students despite the absence of living animals. Is that an academic paradox? Anat Histol Embryol, 36, 220-224.
27. 27. Turan E, Gules O, Kilimci FS, et al (2017): The mixture of liquid foam soap, ethanol and citric acid as a new fixative–preservative solution in veterinary anatomy. Ann Anat, 209, 11-17.
28. 28. Vanezis P, Trujillo O (1996): Evaluation of hypostasis using a calorimeter measuring system and its application to assessment of the post-mortem interval (time of death). Forensic Sci Int, 78, 19-21.
29. 29. Winkelmann A (2007): Anatomical dissection as a teaching method in medical school: A review of the evidence. Med Educ, 41, 15-22.