Serum sialic acid fractions in Kangal breed bitches with transmissible venereal tumours

Year 2008, Volume: 55 Issue: 2, 95 - 98, 01.09.2008

Güneş Serin Funda Kıral İlker Serin

https://doi.org/10.1501/Vetfak_0000000309

Abstract

The serum sialic acid concentration has been reported to be a potentially useful as tumour markers in many types of tumoural disease. The purpose of this study was to investigate of the levels of serum total sialic acid (TSA) and lipid-bound sialic acid (LSA) in transmissible venereal tumours (TVT) in Kangal Breed (Turkish Anatolian Shepherd Dog) bitches. Blood samples were collected from seven untreated bitches with TVT (test group) and eight healthy bitches (control group). Serum lipid bound sialic acid levels determination was done by the spectrophotometric procedure of Katopodis with resorcinol. Serum total sialic acid levels determination was done by the spectrophotometric procedure of Sydow. The mean TSA and LSA levels in the treated and control groups were 13.46±0.66 mg/dl (11.82-16.94) and 6.13±0.46 mg/dl (4.42-7.58); 9.15±0.59 mg/dl (6.28-11.48) and 2.26±0.24 (1.473.63) mg/dl, respectively. Both of serum levels of TSA and LSA in the untreated TVT dogs (n: 7) were significantly (p<0.001) higher than in the healthy dogs (n:8) The data revealed significant elevation in sialic acid levels in TVT and suggested potential utility of these parameters in diagnosis of the TVT. Results of this study suggest that TSA and LSA levels in sera may use in diagnosis serve as a valuable in these tumoural diseases

Keywords

Canine transmissible venereal tumour , sialic acid , tumour markers

Transmissible venereal tümörlü Kangal ırkı dişi köpeklerde serum sialik asit düzeyleri

Abstract

Çeşitli tümöral hastalıkların tanısı amacıyla serum sialik asit düzeyinden yararlanılmaktadır. Bu çalışmada Kangal ırkı dişi köpeklerde görülen bulaşıcı venereal tümör olgularınında serum TSA ve LSA düzeylerinin araştırılması amaçlanmıştır. Bu amaçla 7 tümörlü hastadan ve 8 sağlıklı köpekten kan örnekleri alındı. Serum lipide bağlı sialik asit düzeyleri Katopodis tarafından bildirilen resorsinol yöntemine göre ölçülürken serum total sialik asit düzeyler Sydow tarafından bildirilen yöntemle spektrofotometrik olarak ölçüldü. Test ve kontrol grubuna ait ortalama TSA ve LSA düzeyleri sırasıyla 13.46±0.66 mg/dl (11.8216.94) ve 6.13±0.46 mg/dl (4.42-7.58); 9.15±0.59 mg/dl (6.28-11.48) ile 2.26±0.24 (1.47-3.63) mg/dl bulunmuştur. Tümör grubunun (n=7) serum TSA ve LSA düzeyleri kontrol grubuna (n=8) oranla anlamlı ölçüde yüksek bulunmuştur. TVT olgularında sialik asit düzeyinin artış göstermesi tanı amaçlı olarak kullanılabilir. Sonuç olarak, serum TSA ve LSA düzeyleri TVT tanısında klinik muayene bulguları ile birlikte değerlendirilebileceği düşünülmektedir

Keywords

Köpek bulaşıcı veneral tümör , sialik asit , tümör belirteci

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