Effects of inulin on rumen metabolism in vitro

Abstract

The objective of this study was to investigate the effects of inulin [as topinambur (Helianthus tuberosus L.) powder] on in vitro rumen fermentation of diets containing hay and pelleted concentrate for sheep (approximately 60:40%) in artificial rumen (the RUSITEC system). The RUSITEC system consisted of six fermentation vessels. Topinambur powder was added at levels of 0.2, 0.4, 0.6, 0.8 and 1.0 g/day into the five fermentation vessels while the last one served as a control (without having any topinambur powder). The results showed that the addition of topinambur powder up to 1.0 g/day reduced NH3-N concentration significantly (p<0.05). However, the other basal parameters of rumen fermentation (pH, short-chain fatty acid production and organic matter digestibility) examined in this study were not significantly affected by the addition of topinambur powder. These results indicated that inulin has the potential to improve ruminal nitrogen utilization

Keywords

• Helianthus tuberosus L.
• inulin
• prebiotic
• rumen
• topinambur powder

Inulinin rumen metabolizması üzerine in vitro etkileri

Abstract

Bu araştırmada, kuru ot ve koyun konsantre pelet yeminden oluşan (60:40) bir rasyonun in vitro fermentasyonunu, inulinin [yer elması (Helianthus tuberosus L.) unu formunda] ne şekilde etkilediğinin ortaya konması amaçlanmıştır. Bu amaçla altı fermenterden oluşan bir RUSITEC sistem kullanılmıştır. Fermenterlerden beşine yer elması unu 0.2, 0.4, 0.6, 0.8 ve 1.0 g/gün dozlarında ilave edilirken, sonuncusuna herhangi bir ekleme yapılmayıp kontrol fermenteri olarak kullanılmıştır. Yer elması ununun 1,0 g/gün dozuna kadar yapılan ilavesi amonyak konsantrasyonunu azaltırken (p<0.05), rumen metabolizmasının diğer temel parametreleri olan pH, günlük uçucu yağ asitleri üretimi ve organik madde sindirilebilirliğinde istatistiksel bir değişikliğe yol açmamıştır. Bu araştırmanın sonuçları inulinin ruminal azot değerlendirilebilirliği üzerine olumlu etkilere sahip olabileceğini göstermektedir

Keywords

• Helianthus tuberosus L.
• inulin
• prebiyotik
• rumen
• yer elması unu

References

1. Bergen, WG, Bates DB (1984): Ionophores: their effect on production efficiency and mode of action. J Anim Sci, 58, 1465–1483.
2. Biggs DR, Hancock KR (1998): In vitro digestion of bacterial and plant fructans and effects on ammonia accumulation in cow and sheep rumen fluids. J Gen Appl Microbiol, 44, 167-171.
3. Böhm A, Kaiser I, Trebstein A, Henle T (2005): Heat- induced degradation of inulin. Eur Food Res Technol, 220, 466–471.
4. Chesson A, Forsberg CW (1988): Polysaccharide degradation by rumen micro-organisms. 251-284. In: PN Hobson (Ed), The Rumen Microbial Ecosystem, Elsevier Applied Science, London and New York.
5. Czerkawski JW, Breckenridge G (1977): Design and development of long-term rumen simulation technique (RUSITEC). Br J Nutr, 38, 271–384.
6. Fischbein L, Kaplan M, Gough M (1988): Fructo- oligosaccharides: a review. Vet Hum Toxicol, 30, 104– 107.
7. Gibson GR, Roberfroid MB (1995): Dietary modulation of the colonic microbiota: introducing the concept of prebiotics. J Nutr, 125, 1401–1412.
8. Havenaar R, Marol-Bonin S, Van Dokkum W, Petitet S, Shaafsma G (1999): Inulin: Fermentation and microbial ecology in the intestinal tract. Food Rev Int, 15, 109–120.

9. Hindrichsen IK, Wettstein HR, Machmüller A, Bach Knudsen KE, Madsen J, Kreuzer M (2006): Digestive and metabolic utilisation of dairy cows supplemented with concentrates characterised by different carbohydrates. Anim Feed Sci Technol, 126, 43-61.
10. Höller H, Breves G, Martens H (1983): Non-protein- nitrogen and reduced phosphorus supply to sheep, I. Phosphorus, volatile fatty acids, NH3 and pH of rumen liquid and daily fluid and phosphorus outlow from the rumen. 81-87. In: Nuclear Technique for Assessing and Improving Ruminant Feeds, IAEA, Vienne.
11. Jorgensen H, Zhao XQ, Theil PK, Gabert VM, Bach Knudsen KE (2003): Energy metabolism and protein balance in growing rats fed different levels of dietary fibre and protein. Arch Anim Nutr, 57, 83–98.
12. Kasperowicz A, Michalowski T (2002): Assessment of the fructanolytic activities in the rumen bacterium Treponema saccharophilum strain S. J Appl Microbiol, 92, 140–146.
13. Lee MRF, Merry RJ, Davies DR, Moorby JM, Humphreys MO, Theodorou MK, MacRae JC, Scollan ND (2003): Effect of increasing availability of water- soluble carbohydrates on in vitro rumen fermentation. Anim Feed Sci Technol, 104, 59-70.
14. Leng RA (1982): Modification of rumen fermentation. 427–453. In: JB Hacker (Ed), Nutritional Limits to Animal Production from Pastures. Farnham Royal, Commonwealth Agricultural Bureaux.
15. Niness KR (1999): Inulin and oligofructose: What are they? J Nutr, 129, 1402-1406.
16. Orskov ER, Hovell FDDeB, Mould F (1980): The use of nylon bag technique for the evaluation of feedstuffs. Trop Anim Prod, 5, 195-213.
17. Öztürk H, Breves G (2005): Effects of the prebiotic inulin on in vitro ruminal microbial metabolism. Proceedings of the Society of Nutrition Physiology, Hohenheim-Stuttgart, Germany, 14, 157.
18. Roberfroid MB, Van Loo JAE, Gibson GR (1998): The bifidogenic nature of chicory inulin and its hydrolysis products. J Nutr, 128, 11–19.
19. Stuck K, Faul K, Hylla S, Stein J, Breves G (1995): The application of a semi-continuous colon simulation technique (COSITEC) for studying the effects of clindamycin on microbial hindgut metabolism. Z. Gastroenterol. 33, 241–246.
20. Wang X, Gibson GR (1993): Effects of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestine. J Appl Bacteriol, 75, 373–380.