Isolation of Clostridium sordellii from abomasum lesions of lambs in Turkey

Abstract

In this study, the isolation of C. sordellii from abomasum lesions of lambs and the confirmation of the isolates by polymerase chain reaction (PCR) was attempted. A total of 298 abomasal specimens (swabs) obtained from lambs of 4 to 8 months of age were investigated. Sampled swabs were inoculated onto blood agar plates and incubated anaerobically. Following the incubation grown colonies were identified according to their morphological and biochemical characteristics. C. sordellii was isolated from 17 of 298 specimens (5.7%). These bacteria were also confirmed to harbour C. sordellii DNA by PCR. In conclusion, the presence of C. sordellii in abomasum lesions was demonstrated in Turkey for the first time with this study

Keywords

• Abomasum lesions
• Clostridium sordellii
• isolation
• lamb
• PCR

Türkiye’deki kuzularda abomazum lezyonlarından Clostridium sordellii izolasyonu

Abstract

Bu çalışmada kuzuların abomazum lezyonlarından Clostridium sordellii izolasyonu ve izolatların polimeraz zincir reaksiyonu ile doğrulanması amaçlandı. Çalışma kapsamında 4-8 aylık yaştaki koyunlardan toplanan 298 abomazumdan alınan svab örnekleri incelendi. Sıvabların kanlı agarlara ekimi gerçekleştirilerek anaerobik ortamda inkube edildi. İnkubasyonu takiben şekillenen koloniler morfolojik ve biyokimyasal özelliklerine göre identifiye edildi. İncelen 298 materyalin 17 (% 5.7)’sinde C. sordellii izole edildi. İzolatlar PCR ile C. sordellii DNA’sı yönünden doğrulandı. Sonuç olarak, bu çalışma ile abomazum lezyonlarından C. sordellii izolasyonu Türkiye’de ilk kez gerçekleştirildi

Keywords

• Abomazum lezyonları
• Clostridium sordellii
• izolasyon
• kuzu
• PCR

References

1. Al-Mashat RR, Taylor DJ (1983a): Bacteria in enteric lesions of cattle. Vet Rec, 112, 5-10.
2. Al-Mashat RR, Taylor DJ (1983b): Clostridium sordellii in enteritis in an adult sheep. Vet Rec, 112, 19.
3. Green GA, Schue V, Monteil H (1995): Cloning and characterization of the cytotoxin L-encoding gene of Clostridium sordellii: homology with Clostridium difficile cytotoxin B. Gene, 161, 57-61.
4. Grossman N, Ron EZ (1975): Membrane-bound DNA from Escherichia coli: extraction by freeze-thaw-lysozyme. FEBS Lett, 54, 327-329.
5. Hibbs CM, Johnson DR, Reynolds K, Harrington RJR (1977): Clostridium sordellii isolated from foals. Vet Med Small Anim Clin, 72, 256-258.
6. Kikuchi E, Miyamoto Y, Narushima S, Itoh K (2002): Design of species-specific primers to identify 13 species of Clostridium harbored in human intestinal tracts. Microbiol Immunol, 46, 353-358.
7. Lewis CJ, Naylor R (1996): Sudden death in lambs associated with Clostridium sordellii infection. Vet Rec, 138, 262.
8. Lewis CJ, Naylor R (1998): Sudden death in sheep associated with Clostridium sordellii. Vet Rec, 142, 417- 421.

9. Morris WE, Uzal FA, Fattorini FR, Terzolo H (2002): Malignant oedema associated with blood-sampling in sheep. Australian Vet J, 80, 280-281.
10. Richards SM, Hunt BW (1982): Clostridium sordellii in lambs. Vet Rec, 111, 22.
11. Uzal FA, Hugenholtz P, Blackall LL, Petray S, Moss S, Assis RA, Miyakawa MF, Carloni G (2003): PCR detection of Clostridium chauvoei in pure cultures and formalin-fixed, paraffin-embedded tissues. Vet Microbiol, 91, 239-248.