Histochemical characterization of glycoproteins in the gills of the carp

Abstract

The characteristics of the mucous cells located in gills of the fish Cyprinus carpio were investigated. Mucous cells were determined from gill arc, gill body, primary and secondary lamellae. Mostly mucous cells had oval-globular shape throughout all regions. Histochemical analysis of the gill of Cyprinus carpio showed that mucous content included glycogene and/or oxidizable dioles (PAS +), neutral or acid-rich (PAS/AB pH 2.5 +), sialic acid residues (KOH/PAS +) and strong acid sulphated (AF +) glycoproteins (GPs). Except these mucosubstances, carboxyl groups and/or with sulphate esters (AB pH 2.5 +) strong sulphate (AB pH 0.5 +) (AF/AB pH 2.5 +), O-sulphate esters (AB pH 1+) glycoproteins were also determined

Keywords

• Cyprinus carpio
• density
• gill
• glycoproteins
• mucous cells

Sazan (Cyprinus carpio) solungaç glikoproteinlerinin histokimyasal özellikleri

Abstract

Cyprinus carpio solungaçlarında yer alan karakteristik mukus hücreleri incelendi. Mukus hücreleri solungaç arklarında, solungaç lamellerinde, primer ve sekonder lamellerde tespit edildi. Tüm bölgeler boyunca mukus hücrelerinin çoğu oval-yuvarlak bir şekle sahipti. Histokimyasal analizler solungaçlardaki mukus hücrelerinin glikojen ya da oksidizable diolleri (PAS +), nötral ya da asitce zengin (PAS/AB pH 2.5+), siyalik asid rezidulleri (KOH/PAS +) ve asitce zengin sulfatlı (AF +) glikoproteinleri içerdiği gösterildi. Bu mukosubstanslar dışında karboksil gruplu ya da sülfat esterli (AB pH 2.5 +) güçlü sülfatlı (AB pH 0.5 +) (AF/AB pH 2.5 +), O-sülfat esterli (AB pH 1+) glikoproteinler tespit edildi

Keywords

• Cyprinus carpio
• glikoproteinler
• mukus hücreleri
• solungaç
• yoğunluk

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