The effects of melatonin on plasma homocysteine and oxidative stress parameters in cisplatin-treated rats

Abstract

The aims of this study were to investigate the effects of cisplatin on nephrotoxicity, plasma homocysteine level and biochemical changes related to oxidative stress and to examine the possible protective role of melatonin administration on these parameters in cisplatin treated rats. In this study, eighteen adult rats were used. The rats were randomly divided into three groups. The first group of rats (control) served as untreated controls andreceived only physiological saline. The second group of rats (CP) was given cisplatin (7 mg/kg) intraperitoneally. Group third of rats received cisplatin (7 mg/kg) and melatonin (10 mg/kg) (CP+Mel) intraperitoneally. Administration of cisplatin to rats induced a marked renal failure, characterized with a significantly increase in plasma creatinine and urea concentrations. While the plasma malondialdehyde level significantly increased, the plasma homocysteine, glutathione levels and glutathione peroxidase, catalase activities significantly decreased in CP group when compared to control group. Although the administration of melatonin to cisplatin treated animals significantly increased the plasma levels of antioxidant enzymes, and decreased markedly the plasma malondialdehyde level, it has no any significant effect on plasma homocysteine level compared to alone cisplatin group. However, melatonin decreased the plasma homocysteine level compared to the control group. This study indicated that melatonin ameliorated the cisplatin-induced oxidative stress, which had the harmful effect on the renal function. However; it was ineffective on plasma homocysteine level in cisplatin-treated rats

Keywords

• Cisplatin
• homocysteine
• melatonin
• nephrotoxicity
• oxidative stress
• rat

Sisplatin uygulanan ratlarda plazma homosistein ve oksidatif stres parametreleri üzerine melatonininetkileri

Abstract

Bu çalışmada sisplatin uygulan ratlarda oksidatif stres ile ilişkili biyokimyasal değişimler, plazma homosistein düzeyi ve nefrotoksisite üzerine melatoninin etkisi araştırılmıştır. Bu çalışmada 18 erişkin rat kullanıldı. Ratlar rasgele 3 gruba ayrıldı. Birinci grup ratlara (kontrol) sadece serum fizyolojik uygulaması yapıldı. Ikinci grup ratlara (CP) intraperitonal olarak sisplatin (7 mg/kg) uygulandı. Üçüncü grup ratlara sisplatin (7 mg/kg) ve melatonin (10 mg/kg) (CP+Mel) intraperitonal olarak uygulandı. Ratlara sisplatin uygulaması plazma kreatin ve üre konsantrasyonlarının önemli derecede artışıyla karakterize belirgin bir renal bozukluk oluşturdu. Kontrol grubuyla karşılaştırıldığında sisplatin grubunda glutasyon peroksidaz ve katalaz aktivitesinde plazma homosistein ve glutasyon düzeylerinde önemli bir azalma görülürken, plazma malondialdehid düzeyinde önemli derecede bir artış belirlenmiştir. Sadece sisplatin verilen ve sisplatin ve melatoninin birlikte verildiği gruplar karşılaştırıldığında, plazma malondialdehid düzeyi önemli derecede azalırken plazma antioksidan enzim aktivitesi artmış plazma homosistein düzeyinde ise bir değişim saptanmamıştır. Ancak kontrol grubuyla karşılaştırıldığında plazma homosistein düzeyini melatoninin düşürdüğü görülmüştür. Bu çalışmada, melatoninin sisplatinin neden olduğu oksidatif stres ve renal bozukluklar üzerine iyileştirici etkisi bulunduğu, plazma homosistein düzeyleri üzerine ise etkisiz olduğu tespit edilmiştir

Keywords

• Homosistein
• melatonin
• nefrotoksisite
• oksidatif stres
• rat
• sisplatin

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