Investigation of serum nitric oxide and malondialdehyde levels in cattle infected with Brucella abortus

Abstract

The objective of this study was to determine serum nitric oxide (NO) and malondialdehyde (MDA) levels in cattle infected with Brucella abortus (B. abortus). Study cohort was comprised of 30 cattle aged between 2-3 years; 20 infected with B. abortus and 10 healthy cattle. B. abortus infection was determined in the animals by Serum Agglutination Test and Multiplex PCR assay. Serum NO level was determined by enzymatic Greiss reaction and MDA level by Yoshioka method. Mean serum NO and MDA levels of cases with confirmed B. abortus were 58.20 ± 6.50 µmol/L and 2.25 ± 0.10 nmol/ml, respectively whereas those of the controls were 38.07 ± 2.40 µmol/L and 1.74 ± 0.25 nmol/ml, respectively. We found that serum NO and MDA levels in infected cattle were significantly higher than healthy control cattle (p < 0.05 and p < 0.01, respectively). In conclusion, the increase in NO level in cattle infected with B. abortus is a result of stimulation of NO synthesis in macrophages by bacterial lipopolysaccharides, and the increase in MDA level is a result of excessive radical production due to brucellosis acting upon membrane lipids and can be used as an indicator of tissue damage

Keywords

• Brucella abortus
• cattle
• malondialdehyde
• nitric oxide

Brucella abortus ile enfekte sığırlarda serum nitrik oksit ve malondialdehit düzeylerinin araştırılması

Abstract

Bu çalışmanın amacı Brucella abortus (B. abortus) ile enfekte sığırlarda serum nitrik oksit (NO) ve malondialdehit (MDA) düzeylerini belirlemektir. Çalışmanın materyalini 20 adet B. abortus ile enfekte ve 10 adet sağlıklı olmak üzere 2-3 yaşlarında 30 adet sığır oluşturdu. B. abortus infeksiyonunun tespiti Serum Aglutinasyon Testi ve Multipleks PCR ile gerçekleştirildi. Serum NO düzeyi enzimatik Greiss reaksiyonu ile MDA düzeyi Yoshioka metodu ile belirlendi. B. abortus ile enfekte sığırlarda serum NO ve MDA düzeyleri sırası ile 58.20 ± 6.50 µmol/L ve 2.25 ± 0.10 nmol/ml, kontrol sığırlarda ise 38.07 ± 2.40 µmol/L ve 1.74 ± 0.25 nmol/ml bulundu. Enfekte sığırlarda serum NO ve MDA düzeylerinin sağlıklı kontrollerden istatistik olarak önemli oranda yüksek olduğu belirlendi (sırası ile, p < 0.05 ve p < 0.01). Sonuç olarak, NO düzeyindeki artışın bakteriyel lipopolisakkaridler ile makrofajlardan NO sentezinin uyarılmasının ve MDA düzeyindeki artışın da brusellozis enfeksiyonunun membran lipidlerini etkileyerek aşırı radikal üretimine neden olmasının sonucu olabileceği ve doku hasarının bir göstergesi olarak kullanılabileceği kanaatine varıldı

Keywords

• Brucella abortus
• malondialdehit
• nitrik oksit
• sığır

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