Serum adenosine diaminase activity and nitric oxide level in cows with trichophytosis

Abstract

Trichophytosis which caused by Trichophyton spp. is an enzootic skin disease. In this study, effects of trichophytosis on serum adenozine deaminase (ADA) and nitric oxide (NO) activities were investigated. Thirty cattles with thrichophytosis and 10 healthy cattles were used in the study. Average ages of the cattle were 1-3 and average body weight was 250 kg. Blood samples were taken from vena jugularis of the animals. Serum ADA activities and NO levels were detected with spectrophotomethric method. Serum ADA activities were detected 12.70 U/L and 24.49 U/L in research and control groups respectively. Statistically difference was important between two groups (p<0.001). NO levels were determined 34.24 µmol/L and 36.17 µmol/L in research and control groups respectively. However, no significant statistically change was determined in this parameter between two groups (p>0.05). Consequently, we thought that, serum ADA levels might be increased by cell mediated immune system which was stimulated by T. verricosum. Although, NO levels were monitored slightly increase in research group. We thought that, this increase related to newly iNOS release and NO producing in macrophages

Keywords

• Adenozine deaminase
• nitric oxide
• trichophytosis

Trikofitozisli ineklerde serum adenozin deaminaz aktivitesi (ADA) ve nitrik oksit (NO) düzeyleri

Abstract

Bu çalışmada trikofitozisli ineklerde serum adenozin deaminaz (ADA) aktivitesinin ve nitrik oksit (NO) seviyesinin nasıl etkilendiği araştırıldı. Çalışmada 1-3 yaşlarında, ortalama 250 kg canlı ağırlığında trikofitozisli olduğu belirlenen 30 adet yerli ırk sığır ile 10 adet sağlıklı yerli ırk sığır kullanıldı. Hayvanların vena jugularisinden kan alınarak serumları çıkartıldı. Serumda ADA aktivitesi ve NO düzeyi spekrofotometrik olarak ölçüldü. Serum ADA aktivitesi kontrol ve trikofitozisli sığırlarda sırasıyla 12.70 U/L ve 24.49 U/L olarak tespit edildi. ADA aktivitesinde gruplar arasındaki farklılık istatistiksel olarak önemli bulundu (p<0.001). NO düzeyleri ise kontrol ve trikofitozisli sığırlarda sırasıyla 34.24 µmol/L ve 36.17 µmol/L ölçüldü. Kontrol ile hastalıklı grup arasında NO değerleri bakımından istatistiki anlamda bir fark bulunamadı (p>0.05). Sonuç olarak serum ADA aktivitesinin T.verricosum tarafından hücre aracılı immun sistemin uyarılması sayesinde artmış olabileceği düşünüldü. Buna karşın araştırma grubunda kontrole göre serum NO seviyesinin ortalamasında relatif olmayan bir artış gözlendi. Bu artış, makrofajlardaki iNOS salınımının ve NO üretiminin yeni başlamış olabileceğini düşündürdü

Keywords

• Adenozin deaminaz
• nitrik oksit
• trikofitozis

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