A comparative study on detection of Bordetella avium antibodies in turkeys by enzyme-linkedimmunosorbent assay, microagglutination and serum plate agglutination tests

Abstract

The aims of this study were to develop a serum plate agglutination test (LAT) antigen and microagglutination (MAT) test antigen for the serologic detection of turkeys that have been exposed to B. avium with respiratory disease symptom and to compare sensitivity of commercial enzyme-linked immunosorbent assay (ELISA) with these tests. All serum samples were collected from commercial turkey enterprises in İzmir, samples from 900 turkeys with symptoms of respiratory disease constituted the material of this study. The seroprevalence was determined as 21.6 %, 13.8 %, 12.1 % for ELISA, LAT, and MAT tests, respectively. As a conclusion, although ELISA was defined as the most sensitive test for screening of B. avium antibodies with this study, LAT and MAT’s could also be used for this purpose

Keywords

• B. avium
• enzyme-linked immunosorbent assay
• plate agglutination test
• microagglutination test

Hindilerde Bordetella avium antikorlarının enzyme-linked immunosorbent assay, mikroaglutinasyon ve lam aglutinasyon testleri ile belirlenmesi üzerine karşılaştırmalı bir araştırma

Abstract

Bu çalışmanın amaçları solunum sistemi hastalığı semptomu bulunan hindilerden B. avium’a maruz kalmış olanların serolojik olarak saptanması için lam aglutinasyon (LAT), mikroaglutinasyon (MAT) test antijenlerinin hazırlanması ve bu testlerin duyarlılıklarının ticari enzyme-linked immunosorbent assay (ELISA) ile karşılaştırılmasıdır. Çalışmanın materyalini İzmir İl’i ve çevresinde bulunan, solunum sistemi problemi gösteren 900 ticari hindiden alınan kan serum örnekleri oluşturmaktadır. Seroprevalens ELISA, LAT ve MAT için sırasıyla % 21.6, % 13.8 ve % 12.1 olarak belirlenmiştir. Sonuç olarak; B. avium’a karşı oluşan antikorların belirlenmesinde en duyarlı testin ELISA olmasına karşın; LAT ve MAT’lerinin de bu amaçla kullanılabileceği tespit edilmiştir

Keywords

• B. avium
• enzyme-linked immunosorbent assay
• lam aglutinasyon testi
• mikroaglutinasyon testi

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