Effect of different diluents on the motility of turkey semen during cryopreservation

Abstract

The aim of this study was to investigate the effect of different extenders on sperm motility in cryopreservation of turkey semen. Semen was collected from five toms by manual massage method twice a week for five weeks. Some spermatological parameters of collected semen was assessed and mean ejaculate volume (ml), sperm motility (%) and sperm concentration (x109/ml) were recorded as 0.28±2.8, 84.8±1.12 and 5.1±0.34 respectively. Pooled semen were divided into four equal parts and diluted 1:3 with extenders (Graham, Glucose, Ringer and Tris + Glucose) containing 10% Dimethyl sulphoxide (DMSO) and equilibrated at 4 oC for 90 min. Samples were frozen at -120 o C on liquid nitrogen vapour for 15 min. Sperm motility after dilution was averaged 74±2.6, 78±2.0, 84±2.2, 62±2.9 % and post-thaw motility was 31±2.4, 40.5±2.8, 17±2.0, 11±1.4 % respectively. According to the obtained results, glucose extender provided better environment for turkey semen undergoing cryopreservation than the others (p

Keywords

• Cryopreservation
• extender
• motility
• semen
• turkey

Hindi spermasının dondurulmasında farklı sulandırıcıların spermatozoa motilitesi üzerine etkisi

Abstract

Bu çalışma, hindi spermasının dondurulmasında farklı sulandırıcıların çözüm sonrasında spermatozoa motilitesi üzerine etkisini araştırmak amacı ile yapılmıştır. Çalışmada kullanılan beş adet hindiden sperma, beş hafta süresince haftada iki kez, masaj yöntemi ile alındı. Hindilerden alınan nativ spermanın bazı spermatolojik özellikleri değerlendirildi ve ortalama ejekülat miktarı (ml), motilite (%) ve yoğunluk (x109/ml) sırasıyla 0.28±2.8, 84.8±1.12 ve 5.1±0.34 olarak belirlendi. Değerlendirmeleri yapılan sperma 4 eşit parçaya bölünerek 1:3 oranında; %10 Dimethyl sulphoxide (DMSO) içeren Graham, Glikoz, Ringer ve Tris+Glikoz sulandırıcıları ile sulandırılıp +4 oC de 90 dakika alışıma tabi tutulduktan sonra, -120 o C de sıvı azot buharında 15 dakikada donduruldu. Sulandırma sonrası ortalama motilite değerleri sırasıyla % 74±2.6, 78±2.0, 84±2.2, 62±2.9 ve çözdürme sonrasında ise % 31±2.4, 40.5±2.8, 17±2.0, 11±1.4 olarak elde edildi. Elde edilen sonuçlara göre glikoz tabanlı sulandırıcının hindi spermasının dondurularak saklanmasında diğer sulandırıcılara göre daha iyi sonuçlar verdiği saptandı (p

Keywords

• Hindi
• motilite
• sperma
• sperma sulandırıcısı
• spermanın dondurulması

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