Pathological findings and demonstration of viral antigen by flourescein antibody technique in naturally infected dogs with canine parvovirus

Abstract

In the present study, the pathological findings were described in 10 dogs clinically suspected with canine parvovirus, and the disease was proved by defining viral antijen in the tissue sections by fluorescein antibody technique. In all the cases histopathological lesions were characteristical for the parvoviral enteritis of the dogs. IF positive reactions were present in the small and large intestines, mesenterial lymph nodes, spleen, liver and heart. Despite the presence of the pathological lesions, viral antigens were not present in the lungs. The intensity of the IF positive reactions and severity of the lesions were inversely proportional. Viral antigens were not present in the small intestines (3 cases), and mesenterial lymph nodes (5 cases) of which severe lesions were evident. In conclusion, it is necessary that immunohistochemical application should be carried out in all the organ for definite diagnosis of the canine parvovirus infection

Keywords

• Canine parvovirus
• immunohistochemistry
• pathology
• polyclonal antibody

Doğal enfekte köpek parvovirus enfeksiyonunda patolojik bulgular ve viral antijenin floresan antikor tekniği ile demonstrasyonu

Abstract

Bu çalışmada klinik olarak köpek parvovirus enfeksiyonu şüpheli 10 adet köpekte karşılaşılan patolojik bulgular tanımlandı ve viral antijenlerin dokulardaki dağılımı indirek floresan antikor tekniği ile belirlenerek hastalığın tanısı kesinleştirildi. Tüm olgularda histopatolojik bulgular, karakteristik parvoviral enteritis lezyonları ile örtüşmekteydi. İmmunofloresan pozitif reaksiyonlara ince ve kalın bağırsaklar, mezenteriyel lenf yumruları, dalak, karaciğer ve kalpte rastlandı. Akciğerlerde patolojik bulgular tanımlanmasına karşın, viral antijen saptanmadı. Pozitif reaksiyonların şiddeti, lezyonların şiddetiyle ters orantılıydı. Şiddetli lezyonların görüldüğü 3 olguda ince bağırsaklarda, 5 olguda da mezenteriyel lenf düğümlerinde viral antijene rastlanmadı. Sonuç olarak, özellikle şiddetli lezyonların görüldüğü olgularda kesin tanının ortaya konabilmesi için, bütün organların immunohistokimyasal olarak incelenmesi gerekmektedir

Keywords

• İmmunohistokimya
• köpek parvovirus
• patoloji
• poliklonal antikor

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