Effect of humate and organic acids supplementation to ration on some haematological parameters in laying hens

Abstract

This study was carried out to determine the influence of supplemental humate and organic acids on some haematologic parameters in laying hens. A study was conducted with total 30 white hens. Hens were assigned into three groups equally. The control group was fed a basal diet without humate and organic acid, and treatment groups were fed either 0.15 humate or 0.20 % organic acids added diets. At the end of 60- day treatment period, samples of blood were collected to determine haematologic values. The comparison among the groups showed that both the humate and organic acids supplementation resulted in significant increases (p

Keywords

• Hematological parameter
• humate
• laying hen
• organic acid

Yumurta tavuklarında rasyona ilave edilen humat ve organik asitlerin bazı hematolojik parametreler üzerine etkisi

Abstract

Bu çalışma, yumurta tavuklarında rasyona ilave edilen humat ve organik asitlerin bazı kan parametreleri üzerine etkisini incelemek amacıyla yapıldı. Çalışmada toplam 30 beyaz yumurta tavuğu kullanıldı. Hayvanlar eşit olarak 3 gruba ayrıldı. Kontrol grubu humat veya organik asit ilave edilmeyen bazal (kontrol) rasyonla beslenirken deneme gruplarından birine % 0.15 humat, diğer deneme grubuna ise % 0.20 oranında organik asit ilave edilen rasyon 60 gün süreyle verildi. Deneme periyodunun sonunda kan örnekleri alınarak hematolojik parametreler değerlendirildi. Gruplar arasında yapılan karşılaştırmada, humat ve organik asit grubunun heterofil oranında kontrol grubuna göre önemli bir artma tespit edildi. Diğer taraftan humat ilavesinin eritrosit sayısı ile hemoglobin miktarında önemli bir artma (p

Keywords

• Hematolojik parametre
• humat
• organik asit
• yumurta tavuğu

References

1. AOAC (1984): Official Methods of Analysis of the Association of Official Analytical Chemists. 14th ed., Arlington, Virginia, USA.
2. Bailey CA, White KE, Domke SI (1996): Evaluation of menefee humate on the performence of broilers. Poult Sci, 75, 84.
3. Banaszkiewick W, Drobnik M (1994): The influence of natural peat and isolated humic acid solution on certain indices of metabolism and of acid-base equilibrium in experimental animals. Rocz Panstw Zakl Hig, 45, 353-60.
4. Buczko W, Malinowska MH, Pietraszek D, Pawlak D, Chabielska E (1993): Influence of topla peat preparation on homeostasis in rats. Acta Pol Pharm, 50, 507- 511.
5. Campo JL, Davila SG (2002): Changes in heterophil to lymphocyte ratios of heat-stressed chicken in response to dietary supplementation of several related stres agents. Arch Geflügelk, 66, 80-84.
6. Ceylan N, Çiftçi İ, Kahraman Z, Mızrak C (2005): Yumurta tavuğu yemlerinde humat bileşiklerinin kullanı- mının performans, yumurta kalitesi, ve bağırsak mikroflorası üzerine etkileri. II. Ulusal Hayvan Besleme Kongresi, Konya.
7. Chong-Hua C, Jun-Jen L, Fung-jou L, Mei-Ling Y, Yashang L, Tien Shang H (2002): The effect of humic acid on the adhesibility of neutrophils. Thromb Res, 108, 67-76.
8. Fuchs V, Kuhnert M, Golbs S, Dedek W (1990): The enteral absorbtion of iron (II) from humic acid-iron complexes in suckling piglets using radiolabelled iron (59Fe). Dtsch Tierarztl Wochenschr, 97, 208-209.

9. Gama N, Oliviera MBC, Santin E, Berchieri A (2000): Supplementation with organic acids in diet of laying hens. Ciencia Rural Santa Maria, 30, 499-502.
10. Gillooly M, Bothwell TH, Torrance JD, Macphail AP, Derman DP, Bezwoda RW, Mills W, Charlton RW (1983): The effects of organic acids, phytates and polyphenols on the absorbtion of iron from vegetables. Br J Nutr, 49, 331-342.
11. Humet (1995): Erişim: http://www.humet.hu/141-klinika- eng.pdf. Erişim tarihi: 26.11.2005.
12. İslam KMS, Schumacher A, Groop JM (2005): Humic acid substances in animal agriculture. Pakistan J Nutr, 4, 126-134.
13. Joone GK, Dekker J, van Rensburg CE (2003): Investigation of the immunostimulatory properties of oxihumate. Z Naturforsch, 58, 263-267.
14. Klocking R (1980): Intoxication and detoxication of heavy metals by humic acids. Arch Exp Veterinarmed, 34, 389- 393.
15. Konuk T (1981): Pratik Fizyoloji, 2. baskı, Ankara Üni- versitesi Veteriner Fakültesi Yayınları, Ankara.
16. Laub R (2000): Laub developing humate with anti-HIV, HSV, HPV and other antiviral activity. Erişim: www.laubbiochem.com/pdf/AABullVol13reprint.pdf. Eri- şim tarihi: 09.11.2005.
17. Mueller J, Brun R, Buerki H, Keller HU, Hess MW, Cottier H (1975): Nonspesific acid esterase activity: a criterion for differentiation of T and B lymphocytes in mouse lymph nodes. Eu J Immunol, 5, 270-274.
18. Patent JD, Waldroup PW (1988): The use of organic acids in broiler diets. Poult Sci, 67, 1178-1181.
19. Patterson JA, Burkholder KM (2003): Application of prebiotics and probiotics in poultry production. Poult Sci, 82, 627-631.
20. Riede UN, Zeck-Kap G, Freudenberg N, Keller HU, Seubert B (1991): Humate induced activation of human granulacytes. Virchows Arch B Cell Pathol Incl Mol Pathol, 60, 27-34.
21. Santos KD, Rocha M, Wannmacher CMD, Wajner M (1996): The influence oforganic acids on the proliferation of human peripheral lymphocytes activated by concanavalin a and pokeeweed mitogen. Int J Immunopharmacol, 18, 761-769.
22. Schlottfeldt J, Blazina LR, Wannmacher CM, Wajner M (1995): The effect of organic acids on phytohaemagglu- tinin-activated proliferation of human lymphocytes in vitro. Int J Immunopharmacol, 17, 175-182.
23. Schols D, Wutzler P, Klocking R, Helbig B, De Clercq E (1991): Selective inhibitory activity of polyhydroxcarboxylates derived from phenolic compounds against human immunodeficiency virus replication. J Acq Immun Def Synd, 4, 677-685.
24. Skinner JJ, İzat AL, Waldroup PW (1991): Fumaric acids enhances performance of broilerchicks. Poult Sci, 70, 1444-1447.
25. Sumi N, Yoshiko M, Shunsuke F, Richard N, Hiroshi S (1998): Propionic acid stimulates superoxide generation in human neutrophils. Cell Biol Int, 22, 331-333.
26. TeraVita TM (2004): Humates in poultry and stock farming. Erişim: www.teravita.com/humates. Erişim tarihi: 10.11.2005
27. Thomson JL, Hinton M (1997): Antibacterial activity on formic and propionic acids inthe the diet of hens onsalmonellos in the crop. Brit Poult Sci, 38, 59-67.
28. Trckova M, Matlova L, Hudcova H, Faldyna M, Zraly Z, Dvorska L, Beran V, Pavlik I (2005): Peat as a feed supplement for animals: a review. Vet Med Czech, 50, 361-377.
29. Vanbella M, Teller E, Focant M (1990): Probiotics in animal nutrition: a review. Arch Anim Nutr, 40, 453-460.
30. Visser SA (1973): Some biological effects of humic acids in the rat. Acta Biol Et Med Ger, 31, 569-581.
31. Wajner M, Santos KD, Schlottfeldt JL, Rocha MP, Wannmacher MD (1999): Inhibition of mitogen-activated proliferation of human peripheral lymphocytes in vitro by propiyonic acid. Clin Sci, 96, 99-103.
32. Yaman K (1999): Fizyoloji. 3. baskı, Vipaş A.Ş., Bursa. Geliş tarihi: 17.01.2006 / Kabul tarihi: 28.02.2006