The effect of follicle stimulating hormone and human menopausal gonadotropin on in vitro maturation of bovine oocytes

Abstract

The aim of this study was to investigate the effects of FSH (Follicle Stimulating Hormone) and hMG (human Menopausal Gonadotropin) which are both easy to obtain and cheap when compared to other gonadotropins, on in vitro maturation of bovine oocytes. According to hormone added to in vitro maturation media, three groups were formed; Group I (FSH), Group II (hMG) and Group III (Control). After the in vitro maturation, Giemsa staining of oocytes in group I revealed that 14.36% of oocytes were at GV (Germinal Vesicle) stage, 6.38% of oocytes were at M I (Metaphase I) stage and 76.07% of oocytes were at M II stage while 3.19% of oocytes were degenerated. The percentages of oocytes at stages GV, M I, M II and degenerated oocytes in groups II and III were 11.40%, 15.54%, 66.84%, 6.22% and 23.03%, 6.06%, 64.24%, 6.67% respectively. Distribution of oocytes into different stages within the groups was significantly different (p

Keywords

• Bovine
• follicle stimulating hormone
• human menopausal gonadotropin
• in vitro maturation
• oocyte

Sığır oositlerinin in vitro maturasyonuna follikül uyarıcı hormon ve insan menopozal gonadotropininin etkisi

Abstract

Bu çalışmanın amacı; benzer gonadotropinlere göre daha ucuz olan ve kolay bulunabilen FSH (Follikül Uyarıcı Hormon) ve hMG (İnsan Menopozal Gonadotropini) hormonlarının sığır oositlerindeki in vitro maturasyon oranlarına etkisinin araştırılmasıdır. Oositlerin in vitro maturasyonu sırasında vasatlara eklenen hormonlara göre; FSH kullanılan Grup 1, hMG kullanılan Grup 2 ve kontrol grubu (Grup 3) olmak üzere 3 grup oluşturuldu. In vitro maturasyondan sonra, Grup 1’de Giemsa ile boyaması yapılan oositlerin %14.36’sının GV (Germinal Vezikül), %6.38’inin M I (Metafaz I), %76,07’sinin M II döneminde olduğu belirlenirken, %3.19’unun dejenere olduğu tespit edildi. Bu durum 2. ve 3. Grupta sırasıyla %11.40; %15.54; %66.84; %6.22 ve %23.03; %6.06; %64.24; %6.67 olarak belirlendi. Gruplarda oluşan dağılımların birbirinden farklı olduğu tespit edildi (p

Keywords

• Follikül uyarıcı hormon
• insan menapozal gonadotropin
• in vitro maturasyon
• oosit
• sığır

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