Detection of subclinical mastitis by clinical, microbiological and biochemical methods in sheep

Abstract

The aim of this study was to determine the incidence and aetiology of subclinical ovine mastitis in Kırıkkale and to study the changes occurring in the level of chlorine, sodium, ALP, LDH and NA-Gase, SCC and California Mastitis Test (CMT) scores in milk as a result of subclinical mastitis in 200 sheep. Coagulase negative staphylococci were isolated in 5.5% of the milk samples. The period insidence of subclinical mastitis was detected as 6,5%. Milk samples from noninfected udder halves were found to have variable CMT scores. In milk samples bacteriologically positive , SCC was measured as 300.000-1.000.000 and in milk samples bacteriologically negative ≤300.000. In bacteriologically negative milk samples, the sodium and clorine levels were measured as 56.26 and 47.13 mg/dl respectively while they were found to be 78.65 and 72.67 mg/dl in the bacteriological positive milk samples. In milk from healthy udders, ALP, LDH and NA-Gase enzymes activities were measured as 110.12, 65.60 and 23.51 U/L respectively while they were found to be 194.69, 452.23 and 79.38 U/L in milk from subclinical mastitic udders. The difference between the samples from normal and subclinical mastitis for SCC and biochemical parameters were found to be statistically significant at the level of p

Keywords

• Biochemical profiles
• CMT
• ewe
• SCC
• subclinical mastitis

Koyunlarda klinik, mikrobiyolojik ve biyokimyasal metotlar ile subklinik mastitislerin saptanması

Abstract

Bu çalışmanın amacı, koyunlarda subklinik mastitisin insidansını, etiyolojisini ve subklinik mastitis sonucu sütteki klor (Cl-), sodyum (Na+), ALP, LDH ve NA-Gaz düzeyindeki, SHS ve CMT skorlarındaki değişimleri tespit etmektir. Örneklerin %5.5’inde koagulaz negatif stafilokoklar izole edildi. Subklinik mastitisin prevalansı %6.5 seviyesinde belirlendi. Normal örneklerdeki CMT sonuçları değişken bulundu. Somatik hücre sayısı subklinik mastitisli örneklerde 300.000-1.000.000 ve normal örneklerde ≤300.000 saptandı. Subklinik mastitisli örneklerde Na+ ve Cl- düzeyleri sırasıyla 56.26 ve 47.13 mg/dl, normal örneklerde ise 78.65 ve 72.67 mg/dl bulundu. Normal süt örneklerinde ALP, LDH ve NA-Gaz düzeyleri sırasıyla 110.12, 65.60 ve 23.51 U/L, subklinik mastitisli örneklerde sırasıyla 194.69, 452.23 ve 79.38 U/L tespit edildi. Normal ve subklinik mastitisli örneklerin SHS ve biyokimyasal sonuçlarının istatistiki değerlendirmesinde iki grup arasındaki fark p

Keywords

• Biyokimyasal profil
• CMT
• koyun
• SHS
• subklinik mastitis

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