Characterization of Staphylococcus aureus strains isolated from subclinic bovine mastitis by protein patterns, antibiotic resistance and plasmid profile

Abstract

 A total of 50 Staphylococcus aureus strains isolated from bovine subclinical mastitis in Hatay region were
characterized by protein patterns, antibiotic resistance and plasmid profiles. SDS-PAGE analysis of whole-cell protein extracts
belong to S. aureus strains produced patterns containing 17-49 discrete bands with molecular weights of >14.4-<116 kDa. S. aureus
strains clustered into two cluster on the basis of a computer-assisted numerical analysis. The resistance rates of 50 of S. aureus strains
to trimethoprim-sulphamethoxazole, danofloxacin, amoxicillin-clavulanic, sulbactam-ampicillin, erythromycin, lincomycin,
cefoperazone, oxytetracycline, amoxicillin, streptomycin and penicillin were 4 %, 4 %, 10 %, 14 %, 30 %, 34 %, 36 %, 38 %, 50 %,
54 % and 68 %, respectively. Plasmid profiling demonstrated that 47 of the 50 S. aureus strains contained plasmid ranging from 1.7
to >24 kb. 

Keywords

• Mastitis
• plasmid
• protein
• S. aureus

Subklinik inek mastitislerinden izole edilen Staphylococcus aureus suşlarının protein paterni, antibiyotik direnci ve plazmid profili yönünden karakterizasyonu

Abstract

Hatay bölgesindeki subklinik inek mastitislerinden izole edilen toplam 50 Staphylococcus aureus suşunun protein paterni, antibiyotik dirençliliği ve plazmid profili yönünden karakterizasyonu yapıldı. S. aureus suşlarının SDS-PAGE ile analizinde moleküler ağırlığı 116 kDa arasında değişen 17-49 bant elde edildi. Elektroforetik protein bantlarının bilgisayar destekli numerikal analizi sonucunda S. aureus suşları 2 gruba ayrıldı. S. aureus suşları trimethoprim-sülfametoksazol, danofloksasin, amoksisilin-klavulanik asit, sulbaktam-ampisilin, eritromisin, linkomisin, sefoperazon, oksitetrasiklin, amoksisilin, streptomisin ve penisilin’e sırasıyla % 4, % 4, % 10, % 14, % 30, % 34, % 36, % 38, % 50, % 54 ve % 68 oranlarında dirençli bulundu. İncelenen izolatların 47’sinde, büyüklüğü 1.7->24 kb arasında değişen plazmidler saptandı

Keywords

• Mastitis
• plazmid
• protein
• S. aureus

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