Determination of alpha naphthyl acetate esterase activity in peripheral blood leukocytes of camel (Camelus dromedarius)

Abstract

This study was carried out to determine the alpha naphthyl acetate esterase (ANAE) activity of peripheral blood leukocytes in camels. Heparinized blood samples taken from 13 adult camels were used. ANAE stainings were applied at different pH and durations for the determination of ANAE activity. The best reactions against ANAE were obtained for three hours stainings at pH 5.8. The proportion of ANAE positive T lymphocytes were 81.33 %. There was a diffuse granular pozitivity against ANAE staining in monocytes. However negative reactions were observed in erytrocytes and granulocytes

Keywords

• ANAE activity
• camel
• leukocytes

Develerin (Camelus dromedarius) perifer kan lökositlerinde alfa-naftil asetat esteraz aktivitesinin belirlenmesi

Abstract

Bu çalışma, develerde perifer kan lökositlerinin alfa naftil asetat esteraz (ANAE) aktivitesinin belirlenmesi amacıyla yapıldı. Çalışmada materyal olarak 13 adet erişkin deveden alınan heparinize kan örnekleri kullanıldı. ANAE aktivitesinin belirlenmesi için farklı pH ve sürelerde ANAE boyaması uygulandı. ANAE boyamasına karşı en iyi reaksiyon pH 5.8'de yapılan 3 saatlik boyamalardan elde edildi. Kanda ANAE pozitif T lenfosit oranı % 81.33 olarak tespit edildi. ANAE boyamasına karşı monositlerin diffuz granuler pozitivite gösterdikleri, eritrositlerin ve granulositlerin ise negatif reaksiyon verdiği dikkati çekti

Keywords

• ANAE aktivitesi
• deve
• lökosit

References

1. Alaçam E (1995): Dişi kedide reprodüktif özellikler ve üremenin denetlenmesi. Veteriner Cerrahi Derg, 1, 39-42.
2. Arbeiter K (1977): Genitalerkrankungen der Katze. Kleintierpraxis, 22, 139-143.
3. Aslan S, Erünal N, Kılıçoğlu Ç, Fındık M, Baştan A, Kaymaz M (1995): Einsatz der Papanicolaou Färbamethode zur Vaginalzytologischen Untersuchung bei der Hündin. Ankara Üniv Vet Fak Derg, 42, 431-439.
4. Christiansen IBJ (1984): Reproduction in the Dog and Cat. 1st Ed. 225-262. Baillier- Tindall, London.
5. Concannon PW, Hodgson B, Lein D (1980): Reflex LH – release in oestrus cats following single and multiple copulations. Biol. Reprod, 23, 111-117.
6. Feldman EC, Nelson RW (1996): Canine and Feline Endocrinology and Reproduction. WB Saunders Company, Philadelphia.
7. Günzel AR, Klugsimon C, Peukert AI (1995): Zum Fortpflanzungsgeschehen der Katze-korrespondierende ovarielle und vaginalzytologische Befunde. Der prakt Tierarzt, 66, 727-734.
8. McDonald LE (1980): Veterinary Endocrinology and Reproduction, Lea & Febiger, Philadelphia.

9. Paape SR, Shille VM, Setd H, Stabenfeldt GH (1975): Luteal activitiy in the pseudopregnant cat. Biol Reprod, 13, 470-474.
10. Papanicolaou GN (1942): A new procedure for staning vaginal smear. CSI, 95, 438-439.
11. Reiter E (1982): Die (smear) Diagnostik und Befunde am inneren Genitale von Katzen mit normalem Zyklusablauf und Gynäkopathien. Doktora Tezi Veterinärmedizinische Universität Wien.
12. Shille VM, and Stabenfeldt, GH (1979): Luteal function in the domestic cat during pseudopregnancy and afer treatment with Prostaglandin F2α. Biol Reprod, 21, 1217- 1223.
13. Volker H (1980): Vaginalzytologische Untersuchungen an der Hauskatze (Felis Domestica) unter besonderer Berücksichtigung der Abgrenzung von anovulatorischem und gravidem Zyklus. Doktora Tezi Tierärztliche Hochschule Hannover.
14. Yeager AE, Concannon PW (1990): Association between the preovulatory luteinizing hormone surge and the early ultrasonografic detection of pregnancy and foetal hearbeats in beagle dogs. Theriogenology, 34, 655-665. Geliş tarihi: 20.04.2004 / Kabul tarihi: 18.06.2004