Köpek derisindeki mast hücreleri: dağılım,yoğunluk, heterojenite ve tespit tekniklerinin etkisi

Abstract

Bu araştırma sağlıklı erişkin köpeklerde farklı deri bölgelerindeki mast hücrelerinin yerleşim yerlerini, morfolojilerini ve sayısal dağılımlarını incelemek amacıyla yapıldı. Çalışmada materyal olarak 10 adet sağlıklı köpeklerin yanak, kulak kepçesi, kaburga ve but bölgelerinden alınan deri örnekleri kullanıldı. Bulgularımız, alcian blue / safranin O ile boyanmasına ve formaldehit tespitine duyarlılığına göre köpek derisinde mast hücre heterojenitesini gösterdi. Mast hücreleri bu parametrelerin dört kombinasyonu ile tanımlandı: Formole duyarlı sadece alcian blue-pozitif granüller içeren mast hücreleri, formole dirençli sadece alcian blue-pozitif granüller içeren mast hücreleri, formole dirençli sadece safranin O-pozitif granüller içeren mast hücreleri ve formole dirençli hem safranin O-pozitif hem de alcian blue-pozitif mikst granüller içeren mast hücreleri. Toluidin blue ile boyanmış kesitlerde dört bölgede de IFAA tespitli dokuların % 10 formolle tespit edilenlere göre daha fazla mast hücresi içerdiği dikkati çekti. Bu iki tespit arasında mm²’de mast hücre sayı ortalamalarındaki farklılığın istatistiksel olarak önemli (p0.05) iken, diğer üç bölgede istatistiksel fark önemli (p

Keywords

• Dağılım
• heterojenite
• köpek derisi
• mast hücresi

Mast cells in the dog skin: distribution, density, heterogeneity and influence of fixation techniques

Abstract

This study was conducted with the aim of examining the location, the morphology, and numerical distribution of mast cells in the different areas of the healthy adult dog skin. Skin samples taken from the cheek, pinna, thorax and thigh areas of 10 healthy dogs were used as the study material. The results show the existence of mast cell heterogeneity in dog skin with respect to staining with alcian blue / safranin O and formaldehyde fixation sensitivity. Mast cells characterized by four combination of these parameters were observed: Mast cells containing only alcian blue-positive granules which were formalin-sensitive, mast cells containing only alcian blue-positive granules which were formalin-resistant, mast cells containing only safranin O-positive granules which were formalin resistant and mast cells containing a mixture of alcian blue-positive and safranin O-positive granules which were formalin resistant. With toluidine blue staining method, it was founded that IFAA fixed tissues were contained more mast cells than those tissues fixed with the 10% formaldehyde in all four regions. The difference in average number of mast cells per mm² between the IFAA and the 10% formaldehyde fixed tissues was statistically significant (p0.05), in the other three areas, statistically significant differences (p

Keywords

• Distribution
• dog skin
• heterogeneity
• mast cell

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