S10 silikon plastinasyon tekniği aşamalarında kas dokusunda meydana gelen renk değişimlerinin kantitatif olarak değerlendirilmesi

Abstract

Anatomi biliminde, ölümden sonra hayvan veya insan bedenlerinde meydana gelen değişikliklerin (koku, renk, elastikiyet) en aza indirilmesini sağlamak için kullanılan birçok koruma tekniği vardır. Bu teknikler arasında, bilinen en modern anatomik örnek hazırlama yöntemlerinden biri, plastinasyon tekniğidir. Bu sebeple bu çalışmada kas dokusuna temel plastinasyon aşamaları uygulandı. Çalışmada, standart silikon plastinasyon tekniğinde son ürün elde edilene kadar geçen her aşamada kas dokusunda meydana gelen renk değişimlerinin kolorimetre cihazıyla sayısal olarak ortaya konulması amaçlandı. Plastinasyonun her aşamasında, kas dokusuna renk analizi yapıldı. İlk aşamadan son aşamaya kadar olan tüm süreç boyunca renk değişimi değerlendirildiğinde; elde edilen son üründeki yeşilden kırmızıya renk değişiminin taze dokuya en yakın olduğu aşamanın; 1. aseton banyosu olduğu gözlemlendi. Maviden sarıya değişimin taze dokuya en yakın olduğu aşamanın ise gaz kürleme ve sertleştirme aşaması olduğu görüldü. Ayrıca plastinatların parlaklık açısından değişimi ele alındığında; taze dokuya en yakın değerleri, vakumla gömme aşamasından sonra kaydedildi. Yapılan bu çalışmayla, bugüne kadar doğal renge yakın olarak tanımlanan plastinatlardaki renk değişimi sayısal verilerle belirlendi. Bununla birlikte bu tez çalışması sonucunda kolorimetre cihazının, sahip olduğu avantajlar sebebiyle, anatomi alanında oldukça etkili bir şekilde kullanılabilir olduğu ortaya konuldu.

Keywords

• Kolorimetri
• plastinasyon
• S10

A quantitative evaluation of color changes occurring in the muscle tissue during the stages of S10 silicone plastination technique

Abstract

There are many preservation techniques that are used to ensure that the changes (odor, color, and elasticity) in the characteristics of the animal or human bodies after death are minimized in the field of anatomy. One of the most modern anatomic specimen preparation methods is the plastination technique. Therefore, primary plastination stages were applied to the muscle tissue in this study. The aim of the current study is to present color changes in muscle tissue quantitatively by using a colorimeter device in every stage of the standard silicone plastination technique until the last product is obtained. Color analyses were performed on the muscle tissue after each stage of the plastination. Throughout the whole process, it was observed that the stage when the value of color change from green to red in the product was the closest to the fresh tissue was the 1st acetone bath. The value of color change from blue to yellow was closest to the fresh tissue at the gas curing and hardening stage. Furthermore, the closest values to the fresh tissue were recorded after the impregnation stage when the variations in plastinates were evaluated in terms of brightness. Color changes in plastinates, which have been described as close to the natural color up to today, were determined through statistical data in this study. Moreover, as a result of this dissertation, it was asserted that colorimeter can be effectively used in the field of anatomy due to the advantages it holds.

Keywords

• Colorimeter
• Plastination
• S10

References

1. Ajayi IE, Shawulu JC, Ghaji A et al (2011): Use of formalin and modified gravity-feed embalming technique in veterinary anatomy dissection and practicals. J Vet Med Anim Health, 3, 79-81.
2. Brenner E (2014): Human body preservation-old and new techniques. J Anat, 224, 316-344.
3. Dejong K, Henry RW (2007): Silicone plastination of biological tissue: Cold-temperature technique BiodurTM S10/S15 Technique and Products. J Int Soc Plastination, 22, 2-14.
4. Eisma R, Wilkinson T (2014): From ‘‘Silent Teachers’’ to Models. PLOS Bio, 12, 1-5.
5. Ezhilarasan S, Jetanthi M, Muthuvel VK (2017): Polyester plastination of human cadaveric specimens. Indian J Clin Anatom, 4, 26-29.
6. Henry RW, Janick L, Henry C (1997): Specimen preparation for silicone plastination. J Int Soc Plastination, 12, 13-17.
7. Huidobro FR, Miguel E, Onega E, et al (2003): Changes in meat quality characteristics of bovine meat during the first 6 days post mortem. Meat Sci, 65, 1439-1446.
8. Jangde S, Arya RS, Paikra S, et al (2015): How to provide a safe working condition for medical students and professionals in the anatomy dissection room. Sch J App Med Sci, 3, 1867-1870.

9. Karabacak A, Aytekin İ, Boztepe S (2012): Determination of fattening performance with some body measurements and carcass traits of Malya lambs at the open sheepfold. Arch Zootechnica, 15, 13-22.
10. Kays SJ (1998): Preharvest factors affecting appearance. Postharvest Biol Tec, 15, 233-247.
11. Kumar M, Kataria SK, Mantri LE, et al (2017): Study of plastination to preserve biological specimen in Western Rajasthan. Int J Appl Res, 3, 229-232.
12. Leo´n K, Mery D, Pedreschi F, et al (2006): Color measurement in L*a*b* units from RGB digital images. Food Res, 39, 1084-1091.
13. Mccreary J, Iliff S, Hermey D (2013): Silicone-based coloration technique developed to highlight plastinated specimens. J Int Soc Plastination, 25, 13-20.
14. Melendez-Martinez AJ, Isabel M. Vicario IM, et al (2005): Instrumental measurement of orange juice colour: a review. J Sci Food Agric, 85, 894-901.
15. O’neill GJ, Pais D, Andrade FF, et al (2013): Improvement of the embalming perfusion method: the innovation and the results by light and scanning electron microscopy. Acta Med Port, 26, 188-194.
16. Oliveria AC, Balaban MO (2006): Comparison of a colorimeter with a machine vision system in measuring color of Gulf of Mexico sturgeon fillets. Appl Eng Agric, 22, 583-587.
17. Oostrom K (1987): Fixation of tissue for plastination: general principles. J Int Soc Plastination, 1, 3-11.
18. Pandit S, Kumar S, Mishra BK (2015): Comparative study of anatomical specimens using plastination by araldite HY103, polypropylene resin, 6170H19 Orthocryl and silicone-a qualitative study. Med J Armed Forces India, 71, 246-253.
19. Pashaei S (2010): A brief review on the history, methods and applications of plastination. Int J Morphol, 28, 1075-1079.
20. Ravi SB, Bhat VM (2011): Plastination: A novel, innovative teaching adjunct in oral pathology. J Oral Maxillofac Pathol, 15, 133-137.
21. Riederer BM (2014): Plastination and its importance in teaching anatomy. Critical points for long-term preservation of human tissue. J Anat, 224, 309-315.
22. Saeed M, Phil M, Rufai AA, et al (2001): Mummification to plastination. Saudi Med J, 22, 956-959.
23. Steinke H, Spanel-Borowski K (2005): Coloured plastinates. Ann Anat, 188, 177-182.
24. Stoyanov J, Georgieva A, Sivrev D (2015): Use of physical and chemical factors in the development of plastination anatomical preparations. Trakia J Sci, 13, 21-22.
25. Turan E, Gules O, Kilimci FS, et al (2017): The mixture of liquid foam soap, ethanol and citric acid as a new fixative–preservative solution in veterinary anatomy. Ann Anat, 209, 11-17.
26. Vanezis P, Trujillo O (1996): Evaluation of hypostasis using a calorimeter measuring system and its application to assessment of the post-mortem interval (time of death). Forensic Sci Int, 78, 19-21.
27. Von Hagens G, Tiedemann K, Kriz W (1987): The Current Potential of Plastination. Anat Embryol, 175, 411-421.
28. Wu D, Sun DW (2013): Colour measurements by computer vision for food quality control-a review. Trends Food Sci Tech, 29, 5-20.