Çam ve Kestane Balı’nın Yara İyileşmesi Üzerine Etkilerinin Araştırılması

Abstract

Bu çalışmada, ülkemize özgü çam balı ve kestane balı içeren krem formülasyonlarınin yaralar üzerine iyileştirici etkilerinin inclenmesi amaçlanmıştır. Çalışmada, herhangi bir uygulama yapılmayan kontrol grubu (A), sadece krem taşıt maddesi uygulanan grup (B), Çam Balı kremi uygulanan grup (C) ve Kestane balı kremi uygulanan grup (D) olmak üzere 4 farklı denek grubu oluşturuldu. Her grupta (n=14) yer alan sıçanların sırt kısmına 10 mm çapında punch aparatı ile tam kat yara oluşturuldu. Farklı gruplarda yer alan sıçanların yarısının deri örnekleri yara oluşumunu takip eden ilk 7. günde alınırken geri kalan yarısının deri örnekleri 14. günde alındı. Alınan bu deri örneklerine histopatoloji incelemesi ve hidroksiprolin analizi yapıldı. Histopatolojik değerlendirme sonucuna göre, kronik yangının, kestane balı kremi uygulanan grupta kontrol grubuna göre önemli derecede azaldığı tespit edildi (P<0.05). Buna ek olarak, granülasyon doku ve granülasyon dokusu/fibroblast olgunlaşmasının da kestane balı kremi uygulanan ve 14. gün alınan deri örneklerinde, kontrol grubuna göre önemli derecede azaldığı belirlendi (P<0.05). Kestane balı kremi uygulanan grubun 7. günde alınan deri örnekleri neovaskülarizasyon değerlerinin, kontrol grubu neovaskülarizasyon değerlerine göre daha düşük değerlerde olduğu gözlendi (P<0.05). Ayrıca, hidroksiprolin düzeyleri çalışma grupları yönüyle değerlendirildiğinde B ve D grupları arasındaki farklılık istatistiksel açıdan önemli bulundu (P<0.05). Sonuçlar bütün halinde ele alındığında, kestane balı içeren kremin yara iyileşmesi üzerine katkısının olumlu yönde olduğu belirtilmiştir.

Keywords

• Apiterapi
• çam balı
• kestane balı
• sıçan
• yara iyileşmesi

Investigation of the effects of Pine and Chestnut Honey on wound healing

Abstract

This study aimed to investigate the effect of creams containing pine and chestnut honey on wound healing in rats. The animals were divided into the following four groups: a control group (A), a group treated with only basic cream ingredients (B), a group treated with pine honey cream (C), and a group treated with chestnut honey cream (D). Full-thickness skin wounds were created on the back of each rat (14 per group) with a 10-mm diameter punch instrument. In each group, skin samples were taken from seven rats on day 7 and from the remaining seven rats on day 14. The effects of the creams on wound healing were assessed by histopathological examination and hydroxyproline assays. The histopathological examination showed that chronic inflammation significantly decreased in group D compared to the control group (P<0.05). Furthermore, the levels of granulation tissue and granulation tissue/fibroblast maturation in skin samples taken on day 14 were also significantly lower in group D compared to the control group (P<0.05). It was observed that the neovascularization values of skin samples taken on day 7 were lower in group D than in the control group (P<0.05). Also, the difference in hydroxyproline levels between groups B and D was statistically significant (P<0.05). The results showed that the cream containing chestnut honey had a positive effect on wound healing.

Keywords

• Apitherapy
• chestnut honey
• pine honey
• rat
• wound healing

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