The effects of SNPs between exons 6-9 in the STAT5A gene on milk yield in Anatolian buffalo

Year 2026, Accepted Papers, 1 - 6

Emsal İbiş , Metin Erdoğan

https://doi.org/10.33988/auvfd.1675808

Abstract

The Signal Transducer and Activator of Transcription 5A (STAT5A) gene plays a crucial role in the development of the mammary gland and milk production by mediating prolactin (PRL) signaling. These functions make STAT5A a promising candidate gene for further research, especially in milk-producing animals. The aim of this study was to determine the relationship between polymorphisms in the 1720 bp segment spanning exons 6-9 of the STAT5A gene and the milk yield of 192 Anatolian buffalo raised in the Afyonkarahisar region. The total of 13 polymorphisms were identified through Sanger sequencing, including five in the 7th intron, seven in the 8th intron, and only one of them in the 8th exon. The relationship between adjusted milk yields and SNPs was analyzed using ANOVA (one-way analysis of variance). The association between milk and SNP 12237 (g+12237 C>T) was located in exon 8 of the STAT5A gene. Therefore, milk yield was found to be significant (P<0.01). Buffaloes with the CC genotype were found to produce less milk than buffaloes with the CT genotype for this SNP. In conclusion, our findings suggest that SNP 12237 can be used in buffalo breeding programs to improve milk yield.

Keywords

Anatolian Buffalo , Milk Yield , SNP , STAT5A

Project Number

Afyon Kocatepe University Scientific Research Project Coordination Unit, 19.SAĞ.BİL.24.

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