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Marine fish parasite, Lernanthropus kroyeri (Copepoda) is a repository of Vibrio anguillarum as evidenced by Loop-Mediated Isothermal Amplification method

Year 2021, Volume: 68 Issue: 3, 297 - 300, 30.06.2021
https://doi.org/10.33988/auvfd.714308

Abstract

Fish parasites act as a vector of other pathogenic diseases such as bacteria and virus in fish. Although it has been long known that fish parasites can act as a vector for bacterial pathogens, their role in the transmission of specific bacterial pathogens via particular parasites in the off-host stage has been neglected. The aim of the present study was to disclose that if the copepod parasite, Lernanthropus kroyeri in the off-host stage, can store up the aquatic bacterial pathogens: Aeromonas hydrophila, Photobacterium damsela subsp. piscicida, Vibrio (Listonella) anguillarum, V. harveyi, V. vulnificus, and V. alginolyticus. Our results using the Loop-mediated Isothermal Amplification (LAMP) method demonstrated that the bacteria, V. anguillarum is internally present in the off-host copepod parasites, L. kroyeri. It was also found that the parasite, L. kroyeri could survive in the off-host stage for a given time with its potential of vectoring ability in terms of transmission of the bacterium to the fish. This study demonstrates evidence that the copepod parasite in the off-host stage on the gills of sea bass is a reservoir of pathogen bacteria, Vibrio anguillarum.

Thanks

We thank Agro-Mey Company (Turkey) for providing fish.

References

  • Aartse A, Scholtens IMJ., van der AHJG, et al (2017): Evaluation of a loop-mediated isothermal amplification (LAMP) method for rapid on-site detection of horse meat. Food Control, 81, 9-15.
  • Bandilla M, Valtonen ET, Suomalainen LR, et al (2006): A link between ectoparasite infection and susceptibility to bacterial disease in rainbow trout. Int J Parasitol, 36, 987-991.
  • Barker DE, Braden LM, Coombs MP, et al (2008): Little bugs with smaller bugs: preliminary studies on the role of sea lice as a vector of bacterial pathogens. Aquaculture Canada 2008. Proceedings of the contributed papers of the 25th Annual Meeting of the Aquaculture Association of Canada, Saint John, NB, 11-14 May 2008.
  • Cusack R, Cone DK (1986): A review of parasites as vectors of viral and bacterial diseases of fish. J Fish Diseases, 9, 169-171.
  • Er A, Kayiş Ş (2015): Intensity and prevalence of some crustacean fish parasites in Turkey and their molecular identification. Turkish J Zool, 39, 1142-1150.
  • Gunimaladevi I, Kono T, LaPatra SE, et al (2005): A loop mediated isothermal amplification (LAMP) method for detection of infectious hematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss). Arch Virology, 150, 899-909.
  • Frans I, Michiels CW, Bossier P, et al (2011): Vibrio anguillarum as a fish pathogen: virulence factors, diagnosis and prevention. J Fish Diseases, 34, 643-661.
  • He J, Shi X, Yu L, et al (2013): Development and evaluation of a loop-mediated isothermal amplification assay for diagnosis of Cyprinid herpesvirus 2. J Virol Methods, 194, 206-210.
  • Kotob MH, Menanteau-Ledouble S, Kumar G, et al (2016): The impact of co-infections on fish: a review. Veterinary Research, 47, 1-12.
  • Pylkkö P, Suomalainen LR, Tiirola M, et al (2016): Evidence of enhanced bacterial invasion during Diplostomum spathaceum infection in European grayling, Thymallus thymallus (L.). J Fish Diseases, 29, 79-86.
  • Toksen E (2007): Lernanthropus kroyeri van Beneden, 1851 (Crustacea: Copepoda) infections of cultured sea bass (Dicentrarchus labrax L.). Bull Eur Assoc Fish Pathol, 27, 49-53.
  • Tsai MA, Wang PC, Yoshida S, et al (2019): Establishment of loop-mediated isothermal amplification for rapid and convenient detection of Mycobacterium marinum complex. J Microbiol Methods, 164, 105671.
  • Tully O, Nolan DT (2002): A review of the population biology and host–parasite interactions of the sea louse Lepeophtheirus salmonis (Copepoda:Caligidae). Parasitology, 124, 165-182.
  • Walker PD, Russon IJ, Duijf R, et al (2011): The off-host survival and viability of a native and non-native fish louse (Argulus, Crustacea: Branchiura). Curr Zool, 57, 828-835.
  • Xu DH, Shoemaker CA, Klesius PH (2012): Ichthyophthirius multifiliis as a potential vector of Edwardsiella ictaluri in channel catfish. FEMS Microbiol Lett, 329, 160-167.
  • Yardımci B, Pekmezci G (2012): Gill histopathology in cultured sea bass (Dicentrarchus labrax (L.) co- infected by Diplectanum aequans (Wagener, 1857) and Lernanthropus. Ankara Univ Vet Fak Derg, 59, 61-64.
Year 2021, Volume: 68 Issue: 3, 297 - 300, 30.06.2021
https://doi.org/10.33988/auvfd.714308

Abstract

References

  • Aartse A, Scholtens IMJ., van der AHJG, et al (2017): Evaluation of a loop-mediated isothermal amplification (LAMP) method for rapid on-site detection of horse meat. Food Control, 81, 9-15.
  • Bandilla M, Valtonen ET, Suomalainen LR, et al (2006): A link between ectoparasite infection and susceptibility to bacterial disease in rainbow trout. Int J Parasitol, 36, 987-991.
  • Barker DE, Braden LM, Coombs MP, et al (2008): Little bugs with smaller bugs: preliminary studies on the role of sea lice as a vector of bacterial pathogens. Aquaculture Canada 2008. Proceedings of the contributed papers of the 25th Annual Meeting of the Aquaculture Association of Canada, Saint John, NB, 11-14 May 2008.
  • Cusack R, Cone DK (1986): A review of parasites as vectors of viral and bacterial diseases of fish. J Fish Diseases, 9, 169-171.
  • Er A, Kayiş Ş (2015): Intensity and prevalence of some crustacean fish parasites in Turkey and their molecular identification. Turkish J Zool, 39, 1142-1150.
  • Gunimaladevi I, Kono T, LaPatra SE, et al (2005): A loop mediated isothermal amplification (LAMP) method for detection of infectious hematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss). Arch Virology, 150, 899-909.
  • Frans I, Michiels CW, Bossier P, et al (2011): Vibrio anguillarum as a fish pathogen: virulence factors, diagnosis and prevention. J Fish Diseases, 34, 643-661.
  • He J, Shi X, Yu L, et al (2013): Development and evaluation of a loop-mediated isothermal amplification assay for diagnosis of Cyprinid herpesvirus 2. J Virol Methods, 194, 206-210.
  • Kotob MH, Menanteau-Ledouble S, Kumar G, et al (2016): The impact of co-infections on fish: a review. Veterinary Research, 47, 1-12.
  • Pylkkö P, Suomalainen LR, Tiirola M, et al (2016): Evidence of enhanced bacterial invasion during Diplostomum spathaceum infection in European grayling, Thymallus thymallus (L.). J Fish Diseases, 29, 79-86.
  • Toksen E (2007): Lernanthropus kroyeri van Beneden, 1851 (Crustacea: Copepoda) infections of cultured sea bass (Dicentrarchus labrax L.). Bull Eur Assoc Fish Pathol, 27, 49-53.
  • Tsai MA, Wang PC, Yoshida S, et al (2019): Establishment of loop-mediated isothermal amplification for rapid and convenient detection of Mycobacterium marinum complex. J Microbiol Methods, 164, 105671.
  • Tully O, Nolan DT (2002): A review of the population biology and host–parasite interactions of the sea louse Lepeophtheirus salmonis (Copepoda:Caligidae). Parasitology, 124, 165-182.
  • Walker PD, Russon IJ, Duijf R, et al (2011): The off-host survival and viability of a native and non-native fish louse (Argulus, Crustacea: Branchiura). Curr Zool, 57, 828-835.
  • Xu DH, Shoemaker CA, Klesius PH (2012): Ichthyophthirius multifiliis as a potential vector of Edwardsiella ictaluri in channel catfish. FEMS Microbiol Lett, 329, 160-167.
  • Yardımci B, Pekmezci G (2012): Gill histopathology in cultured sea bass (Dicentrarchus labrax (L.) co- infected by Diplectanum aequans (Wagener, 1857) and Lernanthropus. Ankara Univ Vet Fak Derg, 59, 61-64.
There are 16 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Short Report
Authors

Hijran Yavuzcan 0000-0001-6567-7467

Özge Otgucuoğlu 0000-0002-1432-4693

Publication Date June 30, 2021
Published in Issue Year 2021Volume: 68 Issue: 3

Cite

APA Yavuzcan, H., & Otgucuoğlu, Ö. (2021). Marine fish parasite, Lernanthropus kroyeri (Copepoda) is a repository of Vibrio anguillarum as evidenced by Loop-Mediated Isothermal Amplification method. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 68(3), 297-300. https://doi.org/10.33988/auvfd.714308
AMA Yavuzcan H, Otgucuoğlu Ö. Marine fish parasite, Lernanthropus kroyeri (Copepoda) is a repository of Vibrio anguillarum as evidenced by Loop-Mediated Isothermal Amplification method. Ankara Univ Vet Fak Derg. June 2021;68(3):297-300. doi:10.33988/auvfd.714308
Chicago Yavuzcan, Hijran, and Özge Otgucuoğlu. “Marine Fish Parasite, Lernanthropus Kroyeri (Copepoda) Is a Repository of Vibrio Anguillarum As Evidenced by Loop-Mediated Isothermal Amplification Method”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68, no. 3 (June 2021): 297-300. https://doi.org/10.33988/auvfd.714308.
EndNote Yavuzcan H, Otgucuoğlu Ö (June 1, 2021) Marine fish parasite, Lernanthropus kroyeri (Copepoda) is a repository of Vibrio anguillarum as evidenced by Loop-Mediated Isothermal Amplification method. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68 3 297–300.
IEEE H. Yavuzcan and Ö. Otgucuoğlu, “Marine fish parasite, Lernanthropus kroyeri (Copepoda) is a repository of Vibrio anguillarum as evidenced by Loop-Mediated Isothermal Amplification method”, Ankara Univ Vet Fak Derg, vol. 68, no. 3, pp. 297–300, 2021, doi: 10.33988/auvfd.714308.
ISNAD Yavuzcan, Hijran - Otgucuoğlu, Özge. “Marine Fish Parasite, Lernanthropus Kroyeri (Copepoda) Is a Repository of Vibrio Anguillarum As Evidenced by Loop-Mediated Isothermal Amplification Method”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 68/3 (June 2021), 297-300. https://doi.org/10.33988/auvfd.714308.
JAMA Yavuzcan H, Otgucuoğlu Ö. Marine fish parasite, Lernanthropus kroyeri (Copepoda) is a repository of Vibrio anguillarum as evidenced by Loop-Mediated Isothermal Amplification method. Ankara Univ Vet Fak Derg. 2021;68:297–300.
MLA Yavuzcan, Hijran and Özge Otgucuoğlu. “Marine Fish Parasite, Lernanthropus Kroyeri (Copepoda) Is a Repository of Vibrio Anguillarum As Evidenced by Loop-Mediated Isothermal Amplification Method”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol. 68, no. 3, 2021, pp. 297-00, doi:10.33988/auvfd.714308.
Vancouver Yavuzcan H, Otgucuoğlu Ö. Marine fish parasite, Lernanthropus kroyeri (Copepoda) is a repository of Vibrio anguillarum as evidenced by Loop-Mediated Isothermal Amplification method. Ankara Univ Vet Fak Derg. 2021;68(3):297-300.